بررسي اثرات محافظتي اسانس گياه آويشن شيرازي (Zataria multiflora) بر سميت سلولي القاشده توسط بتا آميلوئيد در سلول‌هاي PC12

The protective effect of Zataria multiflora on the amyloid β25-35-induced cytotoxicity in PC12 Cells

زمينه و هدف: در مطالعه قبلي اثر اسانس آويشن شيرازي و تركيبات اصلي آن، تيمول و كارواكرول بر نقص يادگيري ناشي از بتا آميلوئيد در بهبود عملكرد ادراكي در مدل آلزايمر در موش صحرايي مشاهده شد. در اين مطالعه، اثر اسانس آويشن شيرازي بر سميت سلولي و راديكال­ هاي آزاد اكسيژن ايجادشده توسط بتا آميلوئيد در سلول­ هاي PC12 به عنوان مدل سلولي آلزايمر ارزيابي گرديد. روش‌ها: ابتدا سلول­هاي PC12 به مدت 48 ساعت در مجاورت Aβ25-35 و غلظت ­هاي مختلف آويشن شيرازي قرار داده شدند. سپس بررسي توانايي زيستي سلول­ ها با روش MTT ميزان توليد راديكال­هاي آزاد اكسيژن توسط دستگاه فلوريمتري انجام شد. يافته‌ها: نتايج به­ دست­‌آمده نشان دادند رقت‌­هاي 18/75، 37/5 و 75 ميكروليتر بر ليتر اسانس آويشن شيرازي درصد زنده‌­بودن سلول­ ها را در مقايسه با بتا آميلوئيد به صورت معني­‌داري افزايش داده‌­اند (0/001

Background and aims: In the previous study, the effects of Zataria multiflora (ZM) and its main constituents carvacrol and thymol were evaluated and it was found that they could alleviate cognitive impairments caused by amyloid β (Aβ) in rodent model of Alzheimer’s disease. In this study, the effect of ZM against Aβ25-35-induced cytotoxicity and reactive oxygen species (ROS) accumulation in PC12 cells as a model of Alzheimer's disease was evaluated. Methods: First, PC12 cells were exposed to Aβ25-35 and different concentrations of ZM for 48 h. Then, the cell viability was determined by methyl thiazolyl tetrazolium (MTT) method. Fluorospectrometer was employed to observe ROS production. Results: The results indicated that concentrations of 18.75, 37.5 and 75 μl/L of ZM could significantly rescue and protect PC12 cells against Aβ25-35-induced cytotoxicity (p< 0.001). Furthermore, the data demonstrate that Aβ25-35 induced intracellular ROS, while the concentrations of 18.75 (p <0.05), 37.5 and 75 (p <0.001) µL of ZM could significantly reverse Aβ25-35 induction of ROS generation. Conclusion: According to these findings, ZM could attenuate the level of intracellular ROS induced by Aβ. Therefore, ZM could have antioxidant activity. Ability of ZM to reduce ROS and its protective effects against Aβ-induced cellular damage in PC12 cells may indicate its potential for treatment of Alzheimer’s disease. Further studies are required to determine its clinical efficacy.