عنوان مقاله :
Introducing two isolates of Bdellovibrio from Iran-Rafsanjan
عنوان به زبان ديگر :
فاقد عنوان فارسي
پديد آورندگان :
Heidari Tajabadi, Fereshteh Department of Plant Protection - University of Tehran, Iran , Salehi Juzani, Gholamreza (Department of Microbial Biotechnology - Agricultural Biotechnology Research Institute of Iran (ABRII , Khodaygan, Pejman Department of Plant Protection - University of Valie asr, Rafsanjan, Iran , Ahmadzadeh, Masoud Department of Plant Protection - University of Tehran, Iran
كليدواژه :
Predatory bacteria , Bdellovibrio bacteriovorus , prey range , biological control and 16S rDNA
چكيده فارسي :
فاقد چكيده فارسي
چكيده لاتين :
Bdellovibrio bacteriovorus is an obligate predator of other Gram-negative bacteria. It grows in the periplasm of the prey cell and finally lyses them, so it has high potentials to be used in biocontrol of plant pathogenic bacteria. The objective of the present study was to isolate and characterize Bdellovibrios bacteria from soil and water samples of Rafsanjan, Kerman province, Iran. To do this, after sampling, two Bdellovibrios isolates (Sar and Pj) were isolated by using Escherichia coli strain K12 as prey on the two layer agar. The isolates were identified by phenotypic and 16S rDNA sequencing. The sequencing and phylogenetic studies showed that the strains have more than 99% similarity to B. bacteriovorus. Then, the host range and specificity of the strain were evaluated on 9 different Gram negative bacterial species. The results showed that the strains have predatory effects only on E. coli as prey and do not have any effect on Pseudomonas fluorescence.
عنوان نشريه :
كنترل بيولوژيك آفات و بيماري هاي گياهي
