اﺛﺮ آروﻣﺎﺗﺮاﭘﯽ ﺑﺎ اﺳﺎﻧﺲ زﻧﺠﺒﯿﻞ ﺑﺮ واﮐﻨﺶﻫﺎي ﺑﯽ ﺗﺤﺮﮐﯽ ﺗﻮﻧﯿﮏ و ﺑﺮﺧﯽ از ﻣﺘﺎﺑﻮﻟﯿﺖﻫﺎي ﺧﻮﻧﯽ ﺧﺮوسﻫﺎي اﺧﺘﻪ و ﻏﯿﺮاﺧﺘﻪ

Effects of Aromatherapy with Ginger Essential Oil on Tonic Immobility Reactions and some Blood Metabolites of Caponized and Intact Cockerels

زﻣﯿﻨﻪ و ﻫﺪف: ﮔﺰارش ﺷﺪه اﺳﺖ ﮐﻪ آروﻣﺎﺗﺮاﭘﯽ ﺑﺎ اﺳﺎﻧﺲﻫﺎ در ﻣﻮشﻫﺎ ﻣﻨﺠﺮ ﺑﻪ ﺗﻐﯿﯿﺮ ﺑﺮﺧﯽ از ﻣﺘﻐﯿﺮﻫﺎي ﺳﺮﻣﯽ و ﮐﺎﻫﺶ اﺿﻄﺮاب ﻣﯽﺷﻮد. ﺑﻨﺎﺑﺮاﯾﻦ ﻫﺪف از ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ آروﻣﺎﺗﺮاﭘﯽ ﺑﺎ اﺳﺎﻧﺲ زﻧﺠﺒﯿﻞ ﺑﺮﻣﺘﻐﯿﺮﻫﺎي ﺧﻮﻧﯽ و ﺗﺮس اﻟﻘﺎ ﺷﺪه در ﺧﺮوسﻫﺎي اﺧﺘﻪ و ﻏﯿﺮ اﺧﺘﻪ ﺑﻮد. روش ﮐﺎر: در اﯾﻦ ﭘﮋوﻫﺶ از 10 ﺧﺮوس اﺧﺘﻪ و 10 ﺧﺮوس ﻏﯿﺮاﺧﺘﻪ اﺳﺘﻔﺎده ﺷﺪ. ﻧﺼﻒ ﭘﺮﻧﺪﮔﺎن از ﻫﺮ ﮔﺮوه)5 ﺧﺮوس اﺧﺘﻪ و 5 ﺧﺮوس ﻏﯿﺮاﺧﺘﻪ( ﺑﻪ ﻃﻮر اﻧﻔﺮادي در اﺗﺎﻗﮑﯽ ﺗﺤﺖ آروﻣﺎﺗﺮاﭘﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ و ﺳﺎﯾﺮ ﭘﺮﻧﺪهﻫﺎي آزﻣﺎﯾﺸﯽ ﻧﯿﺰ در ﻫﻤﺎن اﺗﺎﻗﮏ در ﻣﻌﺮض ﺑﺨﺎر آب ﻗﺮار داده ﺷﺪﻧﺪ. ﻫﻤﻪ ﭘﺮﻧﺪهﻫﺎ ﺑﻌﺪ از ﺧﺎرج ﻧﻤﻮدن از اﺗﺎﻗﮏ، ﺑﻪ ﻣﺪت 30 ﺛﺎﻧﯿﻪ ﺑﻪ ﻃﻮر ﻣﻌﻠﻖ از ﭘﺎ آوﯾﺰان و ﺑﻼﻓﺎﺻﻠﻪ آزﻣﻮن TI ﺑﺮ روي آنﻫﺎ ﺻﻮرت ﮔﺮﻓﺖ. دو ﻫﻔﺘﻪ ﺑﻌﺪ از اﻧﺠﺎم اﯾﻦ آزﻣﻮن، روﻧﺪ آروﻣﺎﺗﺮاﭘﯽ در ﻫﻤﻪ ﭘﺮﻧﺪهﻫﺎ ﺗﮑﺮار و ﻣﺘﻐﯿﺮﻫﺎي ﻣﺘﺎﺑﻮﻟﯿﮑﯽ ﭘﻼﺳﻤﺎي ﺧﻮن در ﻗﺒﻞ و ﺑﻌﺪ از آروﻣﺎﺗﺮاﭘﯽ ﻣﻮرد ﺳﻨﺠﺶ ﻗﺮار ﮔﺮﻓﺖ. ﯾﺎﻓﺘﻪﻫﺎ: آروﻣﺎﺗﺮاﭘﯽ در ﮔﺮوه ﺧﺮوسﻫﺎي اﺧﺘﻪ ﻣﻨﺠﺮ ﺑﻪ ﮐﺎﻫﺶ ﻣﺪت زﻣﺎن ﺳﭙﺮي ﺷﺪه در ﺑﯽﺗﺤﺮﮐﯽ ﺷﺪ ﮐﻪ دﻻﻟﺖ ﺑﺮ ﺗﺨﻔﯿﻒ ﺗﻨﺶ اﻟﻘﺎ ﺷﺪه در ﺣﯿﻦ ﺟﻤﻊآوري ﻣﯽﻧﻤﺎﯾﺪ. آروﻣﺎﺗﺮاﭘﯽ ﻫﻢﭼﻨﯿﻦ ﻣﻨﺠﺮ ﺑﻪ اﻓﺰاﯾﺶ ﺳﻄﻮح ﮔﻠﻮﮐﺰ، ﭘﺮوﺗﺌﯿﻦ و اوره ﮐﻞ ﭘﻼﺳﻤﺎﯾﯽ ﮔﺮدﯾﺪ. ﻧﺘﯿﺠﻪ¬ﮔﯿﺮي: آروﻣﺎﺗﺮاﭘﯽ اﺣﺘﻤﺎﻻً ﺑﺎ ﻓﻌﺎل ﻧﻤﻮدن ﺳﺎﻣﺎﻧﻪ ﺳﻤﭙﺎﺗﯿﮑﯽ و اﻓﺰاﯾﺶ ﻓﺸﺎر ﺧﻮن، ﻣﻨﺠﺮ ﺑﻪ اﻓﺰاﯾﺶ ﺳﻄﻮح ﮔﻠﻮﮐﺰ، ﭘﺮوﺗﺌﯿﻦ و اوره ﭘﻼﺳﻤﺎﯾﯽ و ﮐﺎﻫﺶ ﺗﻌﺪاد دﻓﻌﺎت ﻻزم ﺑﺮاي اﻟﻘﺎي TI ﺷﺪه اﺳﺖ. ﺑﻨﺎﺑﺮاﯾﻦ اﺳﺘﻔﺎده از آروﻣﺎﺗﺮاﭘﯽ در ﺣﯿﻦ ﺟﻤﻊآوري ﻣﻤﮑﻦ اﺳﺖ ﺑﺮاي ﮐﺎﻫﺶ ﭘﺎﺳﺦﻫﺎي ﻫﺮاسآﻣﯿﺰ در ﺧﺮوسﻫﺎ ﻗﺎﺑﻞ ﺗﻮﺻﯿﻪ ﺑﺎﺷﺪ.

Inroduction & Objective: Aromatherapy with essential oils (EO) in rats has been reported to alter some of the serum variables and reduce anxiety. Therefore, the purpose of this study was to investigate the effect of aromatherapy with ginger EO on serum metabolites and fear related responses in capons and intact cockerels. Materials and Methods: In this study, 10 caponized and 10 sham-operatedcockerelswere used. Half of the poultries in each group (capons=5 andsham-operated cockerels=5) were treated by aromatic EO in a chamber, and the rest of experimental poultries were exposed to water vapor in the same chamber. Eachbird after removal from the chamber was held by both legs and swung into an inverted position for 30 s and thereaftertonic immobility (TI)test was performed on it.Two weeks after this test, the aromatherapy process was repeated in all birds and blood plasma metabolites were measured before and after aromatherapy. Results: Aromatherapy in the caponized group resulted in a decrease in the tonic immobility duration, indicating a decrease in fearfulness induced by harvesting process.Moreover, aromatherapy significantly increased plasmalevels of glucose, protein and total urea in cockerels. Conclusion: Aromatherapy is probably by activating the sympathetic system and increase blood pressure, lead to increased levels of glucose, protein and serum urea and reduce the number of inductions required to attain the TI response. Therefore, ginger EOexposure during manual harvesting maybe advisable to reduce fearful responses in cockerels.