بررسي اثر لاكتوباسيلوس پلانتاروم و مانان اليگوساكاريد بر رشد و برخي فاكتورهاي گوارشي ماهي قزل آلاي رنگين كمان (Oncorhynchus mykiss)

Effects of Lactobacillus plantarum and Mannan Oligosaccharide on growth performance and some digestive factors in Oncorhynchus mykiss

زﻣﯿﻨﻪ و ﻫﺪف: رﺷﺪ ﺳﺮﯾﻊ و ﻣﻘﺎوﻣﺖ ﺑﺎﻻ در ﭘﺮورش ﻣﺎﻫﯽ اﻣﺮي ﺑﺴﯿﺎر ﻣﻬﻢ اﺳﺖ، ﻟﺬا ﺑﺮرﺳﯽ ﺗﺮﮐﯿﺒﺎت اﻓﺰودﻧﯽ ﻧﻈﯿﺮ ﭘﺮوﺑﯿﻮﺗﯿﮏ، ﭘﺮي ﺑﯿﻮﺗﯿﮏ و ﺳﯿﻨﺒﯿﻮﺗﯿﮏ ﮐﻪ ﺳﺒﺐ ﺑﻬﺒﻮد رﺷﺪ و اﻓﺰاﯾﺶ ﻣﻘﺎوﻣﺖ در ﮔﻮﻧﻪ ﭘﺮورﺷﯽ ﻫﺪف ﺷﻮد ﺑﺴﯿﺎر ﻣﻮرد ﺗﻮﺟﻪ ﻣﺤﻘﻘﯿﻦ در ﺳﺎل- ﻫﺎي اﺧﯿﺮ ﺑﻮده اﺳﺖ. روش ﮐﺎر: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ اﺛﺮ ﺑﺎﮐﺘﺮي ﻻﮐﺘﻮﺑﺎﺳﯿﻠﻮس ﭘﻼﻧﺘﺎروم)Lactobacillus plantarum( و ﻗﻨﺪ ﻣﺎﻧﺎن اﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪ)Mannan Oligosaccharides( ﺑﺮ رﺷﺪ و ﺑﺮﺧﯽ ﻓﺎﮐﺘﻮرﻫﺎي ﮔﻮارﺷﯽ ﻣﺎﻫﯽ ﻗﺰل آﻻي رﻧﮕﯿﻦ ﮐﻤﺎن، ﺗﻌﺪاد 270 ﻋﺪد ﻣﺎﻫﯽ ﻗﺰل آﻻي رﻧﮕﯿﻦ ﮐﻤﺎن ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزﻧﯽ 28/8 ﮔﺮم ﺑﻪ ﺻﻮرت ﮐﺎﻣﻼً ﺗﺼﺎدﻓﯽ در ﻗﺎﻟﺐ 6 ﺗﯿﻤﺎر ﻣﺨﺘﻠﻒ ﭘﺮوﺑﯿﻮﺗﯿﮏ، ﭘﺮي ﺑﯿﻮﺗﯿﮏ و ﺳﯿﻨﺒﯿﻮﺗﯿﮏ و در 3 ﺗﮑﺮار ﺗﻮزﯾﻊ ﺷﺪﻧﺪ. ﮔﺮوه ﭘﺮوﺑﯿﻮﺗﯿﮏ در دو ﺗﯿﻤﺎر آزﻣﺎﯾﺸﯽ 0/3 و 0/5 ﮔﺮم از ﺑﺎﮐﺘﺮي ﻻﮐﺘﻮﺑﺎﺳﯿﻠﻮس ﭘﻼﻧﺘﺎروم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻏﺬا، ﮔﺮوه ﭘﺮي ﺑﯿﻮﺗﯿﮏ در ﯾﮏ ﺗﯿﻤﺎر آزﻣﺎﯾﺸﯽ ﺑﻪ ﻣﯿﺰان 2 ﮔﺮم ﻣﺎﻧﺎن اﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪ ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻏﺬا و ﮔﺮوه ﺳﯿﻨﺒﯿﻮﺗﯿﮏ در دو ﺗﯿﻤﺎر)0/3 ﮔﺮم ﺑﺎﮐﺘﺮي ﻣﺬﮐﻮر ﺑﻪ ﻫﻤﺮاه 2 ﮔﺮم ﻣﺎﻧﺎن اﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪ ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻏﺬا( و ﺗﯿﻤﺎر)0/5 ﮔﺮم ﺑﺎﮐﺘﺮي ﻣﺬﮐﻮر ﺑﻪ ﻫﻤﺮاه 2 ﮔﺮم ﻣﺎﻧﺎن اﻟﯿﮕﻮﺳﺎﮐﺎرﯾﺪ ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻏﺬا( ﺑﻪ ﻣﺪت 45 روز ﻏﺬادﻫﯽ ﺷﺪﻧﺪ. در ﭘﺎﯾﺎن دوره آزﻣﺎﯾﺶ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﻫﺮ ﯾﮏ از ﺷﺎﺧﺺ ﻫﺎي ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﺗﻌﺪاد 15 ﻋﺪد ﻣﺎﻫﯽ ﺑﻄﻮر ﮐﺎﻣﻼ ﺗﺼﺎدﻓﯽ ﺻﯿﺪ ﮔﺮدﯾﺪه و روش ﻫﺎي ﻣﺨﺘﻠﻒ زﯾﺴﺖ ﺳﻨﺠﯽ، ﮐﺎﻟﺮي ﻣﺘﺮي و ﺑﺮرﺳﯽ ﻣﯿﮑﺮوﺳﮑﻮﭘﯽ ﻣﻮرد ارزﯾﺎﺑﯽ واﻗﻊ ﺷﺪﻧﺪ. ﯾﺎﻓﺘﻪ ﻫﺎ: ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، اﻓﺰاﯾﺶ ﻣﻌﻨﺎدار ﻓﺎﮐﺘﻮرﻫﺎي رﺷﺪ و ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢ ﻫﺎي ﮔﻮارﺷﯽ ﻣﻮرد ﺑﺮرﺳﯽ را ﻣﺘﻌﺎﻗﺐ ﻣﺼﺮف ﺑﺎﮐﺘﺮي ﻻﮐﺘﻮﺑﺎﺳﯿﻠﻮس ﭘﻼﻧﺘﺎروم در دوزﻫﺎي 0/3 و 0/5 ﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﻏﺬا در ﺗﯿﻤﺎرﻫﺎي ﭘﺮوﺑﯿﻮﺗﯿﮏ و ﺳﯿﻨﺒﯿﻮﺗﯿﮏ در ﻗﯿﺎس ﺑﺎ ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﻧﺸﺎن داد )P<0.05(.

Inroduction & Objective: Growth and immunity resistance of fish are the most important factors in aquaculture. therefore, many types of research in probiotics, prebiotics, and synbiotics for improving growth and immunity in fish have been studied in the last years. Materials and Methods: In this study, effects of Lactobacillus plantarum and MOSon growth performance, digestive enzymes activity and intestine morphology of Oncorhynchus mykiss were studied. Juvenile rainbow trout (n=270) in 6 administration groups in 18 tanks were kept and fed with diets containing different doses (0.3 and 0.5 g kg-1 feed) of the probiotic, (2 g kg-1 feed) of prebiotic, (0.3+2 and 0.5+ 2g kg-1 feed of probiotic and prebiotic respectively) and a control group for 45 days. Results: Results showed a positive effect on growth performance and digestive enzymes activity in probiotic (0.3 and 0.5 g kg-1 feed of probiotic) and synbiotic groups compared to the control group(p<0.05).