ﺑﯿﺎن ﻋﻮاﻣﻞ ﻧﺴﺨﻪﺑﺮداري 2-ZFP252 ، MYB3R و AP37 و ﻃﻮل رﯾﺸﻪ در ﺑﺮﻧﺞ ﺗﺤﺖ ﺗﻨﺶ ﺧﺸﮑﯽ

Analysis of transcription factors expression patterns; ZFP252, MYB3R-2 and AP37 and root length in rice under drought stress

ﺑﺮﻧﺞ از ﻣﻬﻤﺘﺮﯾﻦ ﻏﻼت ﮐﺸﻮرﻫﺎي آﺳﯿﺎﯾﯽ اﺳﺖ. ﺑﻪ دﻟﯿﻞ ﻣﺤﺪودﯾﺖ آب اﺳﺘﻔﺎده از ژﻧﻮﺗﯿﭗﻫﺎي ﻣﻘﺎوم ﺑﻪ ﮐﻢ آﺑﯽ و ﻣﻄﺎﻟﻌﻪ ﺳﺎزوﮐﺎرﻫﺎي ﺗﺤﻤﻞ ﺑﻪ ﺧﺸﮑﯽ ﻣﻮرد ﺗﻮﺟﻪ ﭘﮋوﻫﺸﮕﺮان ﻗﺮار ﮔﺮﻓﺘﻪ اﺳﺖ. در اﯾﻦ ﭘﮋوﻫﺶ ﺳﻪ رﻗﻢ ﺑﺮﻧﺞ اﯾﺮاﻧﯽ ﻧﺪا، آﻣﻞ3 و ﺳﻨﮓ ﻃﺎرم ﺗﺤﺖ ﺳﻪ ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﺑﺎ ﺳﻄﺢ آﺑﯿﺎري ﻣﻄﻠﻮب، ﻣﻘﺪار آب ﻗﺎﺑﻞ ﺗﺒﺨﯿﺮ ﺧﺎك ﺑﺮاﺑﺮ ﺑﺎ 0/5 ،1 )ﺗﻨﺶ ﻣﻼﯾﻢ( و 0/2 )ﺗﻨﺶ ﺷﺪﯾﺪ( در ﺷﺮاﯾﻂ ﮔﻠﺨﺎﻧﻪ ﭘﺮورش ﯾﺎﻓﺘﻨﺪ. ﻃﻮل رﯾﺸﻪ و ﺳﻄﺢ ﺑﯿﺎن ژن ﺑﺮﺧﯽ از ﻋﻮاﻣﻞ روﻧﻮﯾﺴﯽ ﺷﺎﻣﻞ 2-MYB3R ZFP252 وAP37 در ﻣﺮﺣﻠﻪ رﺷﺪ روﯾﺸﯽ ﺑﻪ روش رﯾﻞ-ﺗﺎﯾﻢ ﭘﯽ ﺳﯽ آر ﮐﻤﯽ در اﯾﻦ ژﻧﻮﺗﯿﭗﻫﺎ ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺖ. ﻃﻮل رﯾﺸﻪ در رﻗﻢ ﻧﺪا اﻓﺰاﯾﺶ ﻣﻌﻨﯽداري در ﻣﻘﺎﯾﺴﻪ ﺑﺎ رﻗﻢ ﺳﻨﮓ ﻃﺎرم در ﺗﻨﺶ ﺧﺸﮑﯽ ﻣﻼﯾﻢ و ﺷﺪﯾﺪ ﻧﺸﺎن داد اﻣﺎ ﺑﯿﻦ ارﻗﺎم ﻧﺪا و آﻣﻞ 3 ﺗﻔﺎوت ﻣﻌﻨﯽداري ﻣﺸﺎﻫﺪه ﻧﺸﺪ. در ﺗﻨﺶ ﺧﺸﮑﯽ ﻣﻼﯾﻢ در رﻗﻢ ﻧﺪا اﻓﺰاﯾﺶ ﮐﻤﺘﺮ و ﻣﻌﻨﯽ داري در ﺳﻄﺢ ﺑﯿﺎن37 و 2-MYB3R در ﻣﻘﺎﯾﺴﻪ ﺑﺎ رﻗﻢ ﺳﻨﮓ ﻃﺎرم و ﻫﻤﭽﻨﯿﻦ اﻓﺰاﯾﺶ ﻣﻌﻨﯽدار ﮐﻤﺘﺮي در ﺳﻄﺢ ﺑﯿﺎن ZFP وAP37 در ﻣﻘﺎﯾﺴﻪ ﺑﺎ رﻗﻢ آﻣﻞ 3 ﻣﺸﺎﻫﺪه ﺷﺪ. در وﺿﻌﯿﺖ ﺗﻨﺶ ﺷﺪﯾﺪ، رﻗﻢ ﻧﺪا اﻓﺰاﯾﺶ ﮐﻤﺘﺮ و ﻣﻌﻨﯽداري در ﺳﻄﺢ ﺑﯿﺎن ﻫﺮ ﺳﻪ ﻋﺎﻣﻞ ﻧﺴﺨﻪﺑﺮداري در ﻣﻘﺎﯾﺴﻪ ﺑﺎ رﻗﻢ ﺳﻨﮓ ﻃﺎرم ﻧﺸﺎن داد. ﺑﻨﺎﺑﺮاﯾﻦ، رﻗﻢ ﻧﺪا اﺣﺘﻤﺎﻻً ﺑﻪ دﻟﯿﻞ ﻃﻮل رﯾﺸﻪ ﺑﯿﺸﺘﺮ در ﺷﺮاﯾﻂ ﺗﻨﺶ ﺧﺸﮑﯽ رﻗﻢ ﻣﺘﺤﻤﻞ ﺗﺮ و ﮐﺎﻧﺪﯾﺪي ﺟﻬﺖ اﻧﺘﺨﺎب ﻋﻠﯿﻪ ﺗﻨﺶ ﺧﺸﮑﯽ اﺳﺖ اﻣﺎ ﺳﺎزوﮐﺎرﻫﺎي ﻣﺨﺘﻠﻔﯽ در واﮐﻨﺶ ﺑﻪ ﺧﺸﮑﯽ در ﺳﻄﺢ ﺑﯿﺎن ژنﻫﺎي ﭘﺎﺳﺦ دﻫﻨﺪه ﺑﻪ اﯾﻦ ﺗﻨﺶ در ارﻗﺎم ﻣﺨﺘﻠﻒ وﺟﻮد دارد.

Rice is one of the most important crops in Asian countries, because of limitation in water resources, the studies on plant tolerance mechanisms to drought stress and the use of tolerant genotypes are going to be more considered. In current research, three Iranian rice cultivars named Neda, Amol3, and Sang-tarom with different responses to drought stress were used. The rice seedlings were grown in three treatments including control (Fraction of Transpirable Soil Water=1.0), mild drought stress (FTSW=0.5) and severe drought stress (FTSW=0.2) in glasshouse. The root length and the expression levels of three transcription factors, ZFP252, MYB3R-2 and AP37were investigated in vegetative stage of growth by qRT-PCR. Neda showed significant increase (P<0.05) in root length with compared to Sang-tarom in mild and severe drought stress but there were not any significant differences between Neda and Amol3. Neda had less significant increases (P<0.05) in expression levels of ZFP252, MYB3R-2 and AP37with compared to Sang-tarom in mild drought stress and also less significant increases in expression levels of ZFP252 and AP37 with compared to Amol3.In severe drought stress (FTSW=0.2), Neda showed less significant expression levels of all three transcription factors with compared to Sang-tarom. Neda is probably more tolerant line and also a candidate for selection against drought stress due to longer roots but there are different mechanisms in responses to drought stress in genes expression levels in different rice lines