اثر مصرف كافئين بر ساختار هيستومورفومتريك سمينال وزيكول و اپيديديم نوزادان موش صحرايي: يك مطالعه تجربي

The Effects of Caffeine Consumption on Histomorphometric Changes of Seminal Vesicle and Epididymis in Rat Offspring: An Experimental Study

زﻣﯿﻨﻪ و ﻫﺪف: از آﻧﺠﺎﯾﯽﮐﻪ ﮐﺎﻓﺌﯿﻦ ﻣﯽﺗﻮاﻧﺪ ﺑﺎﻋﺚ ﮐﺎﻫﺶ ﺣﺠﻢ ﺑﯿﻀﻪ ﺷﺪه و ﺗﻌﺪاد ﺳﻠﻮلﻫﺎي زاﯾﺎي ﺑﯿﻀﻪ ﻣﻮش ﺻﺤﺮاﯾﯽ را ﮐﺎﻫﺶ ﻣﯽدﻫﺪ. ﻟﺬا ﻫﺪف از ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ﺗﻌﯿﯿﻦ اﺛﺮ ﻣﺼﺮف ﮐﺎﻓﺌﯿﻦ در دوره ﺑﺎرداري و ﺷﯿﺮدﻫﯽ ﻣﻮش ﺻﺤﺮاﯾﯽ ﺑﺮ ﺑﺎﻓﺖ وزﯾﮑﻮل ﺳﻤﯿﻨﺎل و اﭘﯿﺪﯾﺪﯾﻢ و ﻫﻢﭼﻨﯿﻦ ﻣﯿﺰان ﻣﻮاﻟﯿﺪ آنﻫﺎ ﺑﻮد. ﻣﻮاد و روشﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ21 ﻣﻮش ﺻﺤﺮاﯾﯽ ﻣﺎده ﺑﺎردار ﺑﻪ ﮔﺮوهﻫﺎي ﮐﻨﺘﺮل و ﺗﺠﺮﺑﯽ )دو ﮔﺮوه( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﮐﺎﻓﺌﯿﻦ ﻣﺤﻠﻮل در آب، ﻃﯽ دوران ﺑﺎرداري و ﺷﯿﺮدﻫﯽ ﺑﻪ ﮔﺮوهﻫﺎي ﺗﺠﺮﺑﯽ 1 و 2 داده ﺷﺪ )ﺑﻪ ﺗﺮﺗﯿﺐ 26 و 45 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم وزن ﺑﺪن روزاﻧﻪ(. در روز 120 ﭘﺲ از ﺗﻮﻟﺪ، ﭘﻨﺞ ﺳﺮ از ﻣﻮاﻟﯿﺪ ﻧﺮ ﻫﺮ ﮔﺮوه ﺑﻪﺻﻮرت ﺗﺼﺎدﻓﯽ اﻧﺘﺨﺎب ﺷﺪﻧﺪ و ﺳﻤﯿﻨﺎل وزﯾﮑﻮل و اﭘﯿﺪﯾﺪﯾﻢ ﻧﻮزادان ﺧﺎرج و ﭘﺲ از ﺗﻮزﯾﻦ، ﺗﺜﺒﯿﺖ و آﻣﺎدهﺳﺎزي ﻣﻘﺎﻃﻊ ﺑﺎﻓﺘﯽ، ﺗﻐﯿﯿﺮات ﺳﺎﺧﺘﺎري ﺑﺎﻓﺘﯽ ﺑﺮرﺳﯽ ﮔﺮدﯾﺪ. ﺑﺮاي ﻣﻘﺎﯾﺴﻪ دادهﻫﺎي ﮔﺮوهﻫﺎ ﺑﺎ ﯾﮑﺪﯾﮕﺮ از آﻧﺎﻟﯿﺰ وارﯾﺎﻧﺲ ﯾﮏﻃﺮﻓﻪ و آزﻣﻮن ﺗﻌﻘﯿﺒﯽ Tukey اﺳﺘﻔﺎده ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: دوز ﺑﺎﻻي ﮐﺎﻓﺌﯿﻦ، ﻣﯿﺎﻧﮕﯿﻦ ارﺗﻔﺎع اﭘﯽﺗﻠﯿﻮم ﺳﻤﯿﻨﺎل وزﯾﮑﻮل را ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﮐﺎﻫﺶ داد وﻟﯽ اﯾﻦ ﮐﺎﻫﺶ از ﻧﻈﺮ آﻣﺎري ﻣﻌﻨﯽدار ﻧﺒﻮد )0/909=P(. اﻓﺰاﯾﺶ ﺿﺨﺎﻣﺖ ﺟﺪار ﻋﻀﻼﻧﯽ ﺗﻮﺑﻮلﻫﺎ در ﮔﺮوه ﮐﺎﻓﺌﯿﻦ ﺑﺎ دوز ﺑﺎﻻ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل دﯾﺪه ﺷﺪ )0/001

Caffeine decreases the testis volume and the number of spermatogenic cells in seminiferous tubules of rat testis. Therefore, the aim of this study was to assess the impact of caffeine exposure during pregnancy and lactation on seminal vesicle and epididymis in rat offspring. Materials and Methods: In this experimental study, 21female pregnant rats were divided into control and experimental groups (two groups). The experimental groups 1 and 2 received caffeine via drinking water during pregnancy and lactation (26 and 45 mg/kg/bw, respectively). The structural changes in seminal vesicle and epididymis were studied by using histological methods on day 120 of postnatal development (n=5, selected randomly from each group). Data were statistically assessed by one-way ANOVA followed by Tukey’s post-hoc Test. Results: The high dose caffeine treated group decreased the epithelium height of seminal vesicle compared with the control group; however, the differences were not significant (p=0.909). The thickness of the muscle layer of the seminal vesicles in the high dose caffeine treated group increased compared with the control group (p<0.001). Significant decreases in epithelium height of tubules of the epididymis in the high dose caffeine treated group was observed compared with the control group (p=0.047), and the diameter of the tubules of epididymis in the high dose caffeine treated group decreased compared with the control group (p<0.001). Conclusion: The results of this study showed high dose caffeine can induce histomorphological changes in the seminal vesicle and epididymis of rat.