بررسي گروه هاي عاملي تركيبات زيست فعال، توانايي راديكال گيرندگي، فعاليت ضدميكروبي و اثر سميت سلولي عصاره آبي گياه شيشه شور (Callistemon citrinus) بر رده سلولي HT29: يك مطالعه آزمايشگاهي

Investigation of the Functional Groups of Bioactive Compounds, Radical Scavenging Potential, Antimicrobial Activity and Cytotoxic Effect of Callistemon Citrinus Aqueous Extract on Cell Line HT29: A Laboratory Study

زﻣﯿﻨﻪ و ﻫﺪف: ﮐﺎرﺑﺮد ﮔﺴﺘﺮده داروﻫﺎي ﺷﯿﻤﯿﺎﯾﯽ ﺟﻬﺖ درﻣﺎن و ﮐﻨﺘﺮل ﺑﯿﻤﺎريﻫﺎي ﻣﺰﻣﻦ و ﻋﻔﻮﻧﯽ و ﻫﻢﭼﻨﯿﻦ ﺳﺮﻃﺎن ﮐﻮﻟﻮن ﺳﺒﺐ اﻓﺰاﯾﺶ ﺗﻘﺎﺿﺎ ﺑﺮاي ﻣﻮاد داروﯾﯽ و ﻧﮕﻪدارﻧﺪه ﻃﺒﯿﻌﯽ و اﯾﻤﻦ ﺷﺪه اﺳﺖ. ﺑﻨﺎﺑﺮاﯾﻦ ﻫﺪف از اﯾﻦ ﭘﮋوﻫﺶ ﺗﻌﯿﯿﻦ ﮔﺮوهﻫﺎي ﻋﺎﻣﻠﯽ ﺗﺮﮐﯿﺒﺎت ﺗﺸﮑﯿﻞ دﻫﻨﺪه، ﻣﯿﺰان ﻓﻨﻮل و ﻓﻼووﻧﻮﺋﯿﺪ ﮐﻞ، ﺗﻮاﻧﺎﯾﯽ رادﯾﮑﺎل ﮔﯿﺮﻧﺪﮔﯽ، ﻓﻌﺎﻟﯿﺖ ﺿﺪﻣﯿﮑﺮوﺑﯽ و ﺑﺮﻫﻢﮐﻨﺶ ﻋﺼﺎره آﺑﯽ ﺷﯿﺸﻪﺷﻮر )Callistemon citrinus( ﺑﺎ آﻧﺘﯽﺑﯿﻮﺗﯿﮏﻫﺎي راﯾﺞ درﻣﺎﻧﯽ و اﺛﺮ ﺳﻤﯿﺖ ﺳﻠﻮﻟﯽ آن ﺑﺮ رده ﺳﻠﻮﻟﯽ HT29 ﺑﻮد. ﻣﻮاد و روشﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ آزﻣﺎﯾﺸﮕﺎﻫﯽ، ﺣﻀﻮر ﮔﺮوهﻫﺎي ﻋﺎﻣﻠﯽ ﺗﺮﮐﯿﺒﺎت ﺗﺸﮑﯿﻞ دﻫﻨﺪه ﻋﺼﺎره آﺑﯽ ﺷﯿﺸﻪﺷﻮر ﺑﺎ ﮐﻤﮏ Fourier transform infrared spectroscopy (FTIR) ﺑﺮرﺳﯽ ﮔﺮدﯾﺪ. ﺗﻮاﻧﺎﯾﯽ ﮔﯿﺮﻧﺪﮔﯽ رادﯾﮑﺎلﻫﺎي آزاد، ﻣﯿﺰان ﻓﻨﻮل و ﻓﻼووﻧﻮﺋﯿﺪ ﮐﻞ، ﻓﻌﺎﻟﯿﺖ ﺿﺪﻣﯿﮑﺮوﺑﯽ و ﺑﺮﻫﻢﮐﻨﺶ ﻋﺼﺎره آﺑﯽ ﺷﯿﺸﻪﺷﻮر ﺑﺎ آﻧﺘﯽﺑﯿﻮﺗﯿﮏﻫﺎي ﮐﻠﺮاﻣﻔﻨﯿﮑﻞ و ﺟﻨﺘﺎﻣﺎﯾﺴﯿﻦ و ﻣﯿﺰان زﻧﺪه ﻣﺎﻧﯽ رده ﺳﻠﻮﻟﯽ HT29 ﺗﺤﺖ ﻏﻠﻈﺖﻫﺎي ﻣﺨﺘﻠﻒ ﻋﺼﺎره ﺑﺎ آزﻣﻮن [4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) MTT-3( ﺑﺮرﺳﯽ ﺷﺪ. دادهﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از آﻧﺎﻟﯿﺰ وارﯾﺎﻧﺲ ﯾﮏﻃﺮﻓﻪ و آزﻣﻮن ﺗﻌﻘﯿﺒﯽ Duncan ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﻋﺼﺎره آﺑﯽ ﺷﯿﺸﻪﺷﻮر ﻓﻌﺎﻟﯿﺖ ﺿﺪﻣﯿﮑﺮوﺑﯽ ﻗﺎﺑﻞ ﺗﻮﺟﻬﯽ ﺑﺮ ﺑﺎﮐﺘﺮيﻫﺎي اﺷﺮﺷﯿﺎ ﮐﻠﯽ، ﺳﻮدوﻣﻮﻧﺎس اﺋﺮوژﯾﻨﻮزا، ﺳﺎﻟﻤﻮﻧﻼ ﺗﯿﻔﯽ، اﺳﺘﺎﻓﯿﻠﻮﮐﻮﮐﻮس اورﺋﻮس و ﻟﯿﺴﺘﺮﯾﺎ اﯾﻨﻮﮐﻮا ﻧﺸﺎن داد. ﻣﯿﺰان ﻣﻬﺎرﮐﻨﻨﺪﮔﯽ رادﯾﮑﺎلﻫﺎي آزاد در ﻣﺤﺪوده 71/68-51/33 درﺻﺪ ﺑﻮد. وﺟﻮد ﮔﺮوهﻫﺎي C-C و OH ﺗﺮﮐﯿﺒﺎت ﭘﻠﯽﻓﻨﻮﻟﯽ ﻋﺼﺎره ﺗﻮﺳﻂ FTIR ﺗﺄﯾﯿﺪ ﺷﺪ. ﻏﻠﻈﺖﻫﺎي ﻣﺨﺘﻠﻒ ﻋﺼﺎره رﺷﺪ ﺳﻠﻮل HT29 را ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﺑﻌﺪ از 24 ﺳﺎﻋﺖ ﮐﺎﻫﺶ داد )P<0/05(. نتيجه گيري عصاره آبي شيشه شور داراي فعاليت ضدميكروبي، پتانسيل آنتي اكسيداني و سميت سلولي بر رده سلولي HT29 بود. بنابراين، استفاده از عصاره آبي شيشه شور در صنايع داروسازي و غذايي پيشنهاد مي گردد.

The extensive use of chemical drugs for the treatment and control of chronic and infectious diseases as well as colorectal cancer, has led to an increased demand for natural and safe drugs and preservatives. Therefore, this study aimed to evaluate the presence of functional groups of constituents, total phenol and flavonoids content, radical scavenging potential, and antimicrobial activity of Callistemon citrinus aqueous extract (CCE). Its interaction with common therapeutic antibiotics and cytotoxic effect on cell line HT29 was also evaluated. Materials and Methods: In this laboratory study, the presence of functional groups of chemical constituents of C. citrinus aqueous extract was evaluated by Fourier transform infrared (FTIR) spectroscopy. Free radical scavenging activity, total phenol and flavonoids content, antimicrobial activity of C. citrinus aqueous extract and its interaction with chloramphenicol and gentamicin antibiotics, and the viability of cell line HT29 (through MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test) under different concentrations of C. citrinus aqueous extract were investigated. Data were analyzed using one-way ANOVA followed by Duncan’s test. Results: The C. citrinus aqueous extract had a considerable antimicrobial effect on Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, and Listeria innocua. The free radicals scavenging effect of C. citrinus aqueous extract was in the range of 51.33-71.68%. The presence of C-C and OH groups of phenolic compounds of C. citrinus aqueous extract was confirmed by FTIR. After 24 hours, the different concentrations of C. citrinus aqueous extract reduced the growth of cell line HT29 compared to the control group (p<0.05). Conclusion: The C. citrinus aqueous extract had antimicrobial activity, antioxidant potential and inhibitory effect on cell line HT29. Therefore, the use of C. citrinus aqueous extract could be suggested in pharmaceutical and food industries