اثر طولاني مدت غذاي پرچرب و تمرين منظم هوازي بر بيان ايزوفرم هاي يك و دو كراتين كيناز ميتوكندري (CKmt1,2) در بافت چربي سفيد موش سوري: يك مطالعه تجربي

The Long-Term Effect of High Fat Diet and Regular Aerobic Exercise Training on Gene Expression of Isoforms of Mitochondrial Creatine Kinase (Ckmt1,2) in White Adipose Tissue of Mice: An Experimental Study

زﻣﯿﻨﻪ و ﻫﺪف: ﮐﺮاﺗﯿﻦ ﮐﯿﻨﺎز ﻣﯿﺘﻮﮐﻨﺪري در ﮔﺮﻣﺎزاﯾﯽ ﻣﺴﺘﻘﻞ از ﭘﺮوﺗﺌﯿﻦ ﻏﯿﺮ ﺟﻔﺖ ﮐﻨﻨﺪه ﻧﻘﺶ دارد. ﺗﺼﻮر ﻣﯽﺷﻮد، اﯾﻦ ﻋﺎﻣﻞ ﺑﺮ اﺛﺮ ﺗﻐﺬﯾﻪ و ورزش ﻣﺘﺄﺛﺮ ﺷﻮد. ﻫﺪف از اﯾﻦ ﭘﮋوﻫﺶ ﺗﻌﯿﯿﻦ اﺛﺮ ﻣﺼﺮف ﻃﻮﻻﻧﯽ ﻣﺪت ﻏﺬاي ﭘﺮﭼﺮب و ﺗﻤﺮﯾﻦ ورزﺷﯽ ﻣﻨﻈﻢ ﻫﻮازي ﺑﺮ ﺑﯿﺎن اﯾﺰوﻓﺮمﻫﺎي ﯾﮏ و دو ﮐﺮاﺗﯿﻦ ﮐﯿﻨﺎز ﻣﯿﺘﻮﮐﻨﺪري )(CKmt1,2 در ﺑﺎﻓﺖ ﭼﺮﺑﯽ ﺳﻔﯿﺪ ﻣﻮش ﺳﻮري ﺑﻮد. ﻣﻮاد و روشﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ، 28 ﺳﺮ ﻣﻮش ﺳﻮري ﻧﺮ ) (C57BL/6 در ﭼﻬﺎر ﮔﺮوه ﮐﻨﺘﺮل )7 ﺳﺮ(، ﻏﺬاي ﭘﺮﭼﺮب )7 ﺳﺮ(، ﺗﻤﺮﯾﻨﯽ )7 ﺳﺮ( و ﻏﺬاي ﭘﺮﭼﺮب- ﺗﻤﺮﯾﻨﯽ )7 ﺳﺮ( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﻣﻮشﻫﺎي ﮔﺮوهﻫﺎي ﻏﺬاي ﭘﺮﭼﺮب ﺑﻪ ﻣﺪت 12 ﻫﻔﺘﻪ، ﺑﺎ ﻏﺬاي ﭘﺮﭼﺮب )40 درﺻﺪ( ﺗﻐﺬﯾﻪ ﺷﺪﻧﺪ. ﻣﻮشﻫﺎي ﮔﺮوهﻫﺎي ﺗﻤﺮﯾﻨﯽ، ﺑﻪ ﻣﺪت ﺷﺶ ﻫﻔﺘﻪ، ﺗﺤﺖ ﺗﻤﺮﯾﻦ ﺗﺪاوﻣﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﺑﺮاي اﻧﺪازهﮔﯿﺮي ﺑﯿﺎن ﻧﺴﺒﯽ ژنﻫﺎي CKmt1 و CKmt2 از روش Real time PCR اﺳﺘﻔﺎده ﺷﺪ. ﺑﺮاي ﺗﺤﻠﯿﻞ آﻣﺎري دادهﻫﺎ از آزﻣﻮن ANOVA دو ﻃﺮﻓﻪ اﺳﺘﻔﺎده ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﻧﺘﺎﯾﺞ اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن داد ﮐﻪ ﻏﺬاي ﭘﺮﭼﺮب )P=0/324( و ﺗﻤﺮﯾﻦ ورزﺷﯽ )0/136=P( ﺗﺄﺛﯿﺮ ﻣﻌﻨﯽداري در ﺑﯿﺎن ژن CKmt1 ﻧﺪارد. ﺑﺎ اﯾﻦ ﺣﺎل، ﺑﯿﺎن ژن CKmt2 ﺑﺮ اﺛﺮ ﻏﺬاي ﭘﺮﭼﺮب، ﮐﺎﻫﺶ ﻣﻌﻨﯽدار )0/043=P( و ﺑﺮ اﺛﺮ ﺗﻤﺮﯾﻦ ورزﺷﯽ اﻓﺰاﯾﺶ ﻣﻌﻨﯽداري )0/001=P( ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﭘﺮﭼﺮب داﺷﺖ. ﻧﺘﯿﺠﻪﮔﯿﺮي: ﯾﺎﻓﺘﻪﻫﺎي اﯾﻦ ﻣﻄﺎﻟﻌﻪ دﻻﻟﺖ ﺑﺮ اﯾﻦ دارﻧﺪ ﮐﻪ ﻣﺼﺮف ﻃﻮﻻﻧﯽ ﻣﺪت ﻏﺬاي ﭘﺮﭼﺮب و ﺗﻤﺮﯾﻦ ﻣﻨﻈﻢ ﺑﻪ ﺗﺮﺗﯿﺐ از ﻃﺮﯾﻖ ﮐﺎﻫﺶ و اﻓﺰاﯾﺶ ﺑﯿﺎن ﮐﺮاﺗﯿﻦ ﮐﯿﻨﺎز ﻣﯿﺘﻮﮐﻨﺪري اﺣﺘﻤﺎﻻً ﻣﯽﺗﻮاﻧﻨﺪ ﮔﺮﻣﺎزاﯾﯽ ﻏﯿﺮﻟﺮزﺷﯽ در ﺑﺎﻓﺖ ﭼﺮﺑﯽ ﺳﻔﯿﺪ را ﻣﺘﺄﺛﺮ ﺳﺎزﻧﺪ.

Mitochondrial creatine kinase involves in UCP-independent thermogenesis. It isthought that the agent can be affected by nutrition and exercise. The aim of this study was to investigate the longterm effect of high fat diet and regular aerobic exercise training on gene expression of mitochondrial creatine kinase 1 (CKmt1) and mitochondrial creatine kinase 2 (CKmt2) in white adipose tissue of mice. Materials and Methods: In this experimental study, 28 male C57BL/6 mice were divide into four groups including control (n=7), high fat diet (HFD) (n=7), exercise training (ET) (n=7) and HFD-exercise training (HFD-ET) (n=7). The subjects of the HFD groups were fed a high-fat diet (fat: %40) for a period of 12 weeks. The subjects of the training groups underwent continuous training for six weeks. The Real Time–PCR method was used to measure the expression levels of the Ckmt1and Ckmt2 genes. The two-way ANOVA was applied to analyze data. Results: Data showed that the HFD (p=0.324) and ET (p=0.136) did not significantly affect the gene expression level of CKmt1. However, the gene expression level of CKmt2 was significantly decreased and increased by HFD and ET versus high fat diet group, respectively (p=0.043; p=0.001, respectively). Conclusion: The findings of this study indicated that the long term feeding of high fat diet and regular aerobic training could probably affect the non-shivering thermogenesis at white adipose tissue by decreasing and increasing the expression of mitochondrial creatine kinase 2 (CKmt2), respectively.