اثرات آنتي اكسيداني و ضد اضطرابي نانوفيتوزوم سماق در موش هاي صحرايي مدل بيماري آلزايمر: يك مطالعه تجربي

Antioxidant and Anti-anxiety Effects of Sumac Nano-Phytosome in Rat Model of Alzheimer’s disease: An Experimental Study

زﻣﯿﻨﻪ و ﻫﺪف: آﻟﺰاﯾﻤﺮ، ﺑﯿﻤﺎري ﺗﺤﻠﯿﻞ ﻋﺼﺒﯽ ﻫﻤﺮاه ﺑﺎ اﺧﺘﻼل رﻓﺘﺎري اﺳﺖ. ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﻌﯿﯿﻦ اﺛﺮات ﺿﺪ اﺿﻄﺮاﺑﯽ و آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﻋﺼﺎره ﺳﻤﺎق و ﻧﺎﻧﻮﻓﯿﺘﻮزوم آن در ﻣﻮشﻫﺎي ﺻﺤﺮاﯾﯽ ﻣﺪل ﺑﯿﻤﺎري آﻟﺰاﯾﻤﺮي اﻟﻘﺎء ﺷﺪه ﺑﺎ اﺳﺘﺮﭘﺘﻮزوﺗﻮﺳﯿﻦ اﺳﺖ. ﻣﻮاد و روشﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ 35 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر در ﭘﻨﺞ ﮔﺮوه ﮐﻨﺘﺮل، ﮐﻨﺘﺮل ﻣﺜﺒﺖ، ﮔﺮوه آﻟﺰاﯾﻤﺮي و ﮔﺮوهﻫﺎي آﻟﺰاﯾﻤﺮي درﯾﺎﻓﺖ ﮐﻨﻨﺪه ﻋﺼﺎره ﺳﻤﺎق و ﻧﺎﻧﻮﻓﯿﺘﻮزوم ﺳﻤﺎق ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﻣﺪل آﻟﺰاﯾﻤﺮ ﺑﺎ ﺗﺰرﯾﻖ درون ﺑﻄﻨﯽ-ﻣﻐﺰي اﺳﺘﺮﭘﺘﻮزوﺗﻮﺳﯿﻦ ﺑﺎ ﻏﻠﻈﺖ 3 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم اﻟﻘﺎء ﺷﺪ. رﻓﺘﺎرﻫﺎي ﺷﺒﻪ اﺿﻄﺮاﺑﯽ21 روز ﺑﻌﺪ از ﮔﺎواژ ﺑﺎ اﺳﺘﻔﺎده از ﺗﺴﺖ ﻣﺎز ﺑﻌﻼوه ﻣﺮﺗﻔﻊ ارزﯾﺎﺑﯽ ﺷﺪﻧﺪ. در اداﻣﻪ، ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢﻫﺎي ﮐﺎﺗﺎﻻز، ﺳﻮﭘﺮاﮐﺴﯿﺪ دﯾﺴﻤﻮﺗﺎز و ﻫﻢﭼﻨﯿﻦ ﺳﻄﺢ ﻣﺎﻟﻮن ديآﻟﺪﻫﯿﺪ در ﻧﺎﺣﯿﻪ ﻗﺸﺮ ﻣﻐﺰ اﻧﺪازهﮔﯿﺮي ﺷﺪ. ﺑﺮاي ﺗﺤﻠﯿﻞ آﻣﺎري از آزﻣﻮن ﺗﺤﻠﯿﻞ وارﯾﺎﻧﺲ ﯾﮏﻃﺮﻓﻪ و آزﻣﻮن درون ﮔﺮوﻫﯽ Tukey اﺳﺘﻔﺎده ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ اﺳﺘﺮﭘﺘﻮزوﺗﻮﺳﯿﻦ ﺳﺒﺐ اﻓﺰاﯾﺶ رﻓﺘﺎرﻫﺎي ﺷﺒﻪ اﺿﻄﺮاﺑﯽ )0/001=p(، ﺳﻄﺢ ﻣﺎﻟﻮن ديآﻟﺪﻫﯿﺪ )0/017=p( و ﮐﺎﻫﺶ ﻓﻌﺎﻟﯿﺖ ﮐﺎﺗﺎﻻز و ﺳﻮﭘﺮاﮐﺴﯿﺪدﯾﺴﻤﻮﺗﺎز ﻗﺸﺮ ﻣﻐﺰ در ﮔﺮوه آﻟﺰاﯾﻤﺮي ﻣﯽﺷﻮد )p<0/001( و ﺗﯿﻤﺎر ﺑﺎ ﻧﺎﻧﻮﻓﯿﺘﻮزوم ﺳﻤﺎق ﺳﺒﺐ ﮐﺎﻫﺶ رﻓﺘﺎرﻫﺎي ﺷﺒﻪ اﺿﻄﺮاﺑﯽ )0/002=p(، اﻓﺰاﯾﺶ ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢﻫﺎي ﮐﺎﺗﺎﻻز و ﺳﻮﭘﺮاﮐﺴﯿﺪ دﯾﺴﻤﻮﺗﺎز و ﮐﺎﻫﺶ ﺳﻄﺢ ﻣﺎﻟﻮن ديآﻟﺪﻫﯿﺪ ﻗﺸﺮ ﻣﻐﺰ )0/003=p( ﮔﺮدﯾﺪ. ﻧﺘﯿﺠﻪﮔﯿﺮي: ﻧﺘﺎﯾﺞ اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن داد ﮐﻪ ﻧﺎﻧﻮ ﻓﯿﺘﻮزوم ﺳﻤﺎق ﺑﻪ دﻟﯿﻞ ﻓﺮاﻫﻤﯽ زﯾﺴﺘﯽ ﺑﻬﺘﺮ، ﻣﻤﮑﻦ اﺳﺖ ﺳﺒﺐ ﮐﺎﻫﺶ رﻓﺘﺎرﻫﺎي ﺷﺒﻪ اﺿﻄﺮاﺑﯽ و اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ اﻟﻘﺎ ﺷﺪه ﺑﺎ اﺳﺘﺮﭘﺘﻮزوﺗﻮﺳﯿﻦ را در ﻣﻮش ﻫﺎي ﺻﺤﺮاﯾﯽ ﻣﺪل ﺑﯿﻤﺎري آﻟﺰاﯾﻤﺮ ﮔﺮدد.

Alzheimer’s disease (AD) is a neurodegenerative disease accompanied by common emotional symptoms such as anxiety and fear. The aim of this study was investigating the anti-anxiety and antioxidant effects of sumac extract and its nano-phytosome, in streptozotocin (STZ)-induced rat model of Alzheimer's disease Materials and Methods: In this experimental study, 35 male Wistar rats were divided into five groups (control, positive control, Alzheimer, and Alzheimer with (40mg/kg) doses of sumac extract and nano-phytosome - sumac). For the induction of Alzheimer model, STZ (3 mg / kg) was injected intracerebroventricularlly. 21 days after gavage, plus maze test was performed to evaluate anxiety behavior in the rats. In the end, catalase and superoxide dismutase enzyme's activity and malondialdehyde levels were measured in the cerebral cortex. Statistical analysis was performed using one-way ANOVA followed by Tukey's test. Results: The results showed that STZ increased anxiety-like behaviors (p=0.001), malondialdehyde (MDA) level (p<0.017), and decreased catalase and superoxide dismutase activity in the Alzheimer group (p<0.001); while treatment with sumac nano-phytosome reduced anxiety-like behaviors (p=0.002), increased catalase and superoxide dismutase activity and decreased MDA level in cerebral cortex (p=0.003). Conclusion: The results suggested that sumac nano-phytosome due to improving the bioavailability may reduce STZinduced anxiety-like behaviors and oxidative stress in rat model of AD.