عنوان مقاله :
مقايسه اثر سه نوع رژيم غذايي پر چربي، پر پروتئين و كم كالري بر بيان ژن هاي Klotho و TGF-β در كبد و سرم خون موش هاي صحرايي نر پير نژاد ويستار
عنوان به زبان ديگر :
Comparison of Effects of High-Fat, High-Protein and Low-Calorie Diets on Klotho Gene Expression and TGF-β Level in Serum and Liver of Old Male Wistar Rats
پديد آورندگان :
اصحابي قربانگل داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺗﻬﺮان - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ - ﮔﺮوه ﻓﯿﺰﯾﻮﻟﻮژي , رحيمي احمد مصطفي داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺗﻬﺮان - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ - ﮔﺮوه ﻓﯿﺰﯾﻮﻟﻮژي , شفيعي آناهيد داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺗﻬﺮان - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ - ﮔﺮوه ﻓﯿﺰﯾﻮﻟﻮژي , نبوي زاده فاطمه داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺗﻬﺮان - داﻧﺸﮑﺪه ﭘﺰﺷﮑﯽ - ﮔﺮوه ﻓﯿﺰﯾﻮﻟﻮژي
كليدواژه :
ﭘﯿﺮي , Klotho , رژﯾﻢ ﻏﺬاﯾﯽ ﭘﺮﭘﺮوﺗﺌﯿﻦ , رژﯾﻢ ﻏﺬاﯾﯽ ﭘﺮﭼﺮﺑﯽ , TGFβ , رژﯾﻢ ﻏﺬاﯾﯽ ﮐﻢ ﮐﺎﻟﺮي
چكيده فارسي :
ﺳﺎﺑﻘﻪ و ﻫﺪف: رژﯾﻢ ﻫﺎي ﻏﺬاﯾﯽ ﻧﻘﺶ ﻣﻬﻤﯽ در ﺳﻼﻣﺖ اﻧﺴﺎن و ﺟﻠﻮﮔﯿﺮي از ﻓﺮاﯾﻨﺪ ﭘﯿﺮي دارد. ﺑﺮرﺳﯽ ﺣﺎﺿﺮ ﻣﯿﺰان ﭘﺮوﺗﺌﯿﻦ ﺿﺪ ﭘﯿﺮي Klotho را در ﺳﺮم ﺧﻮن و ﺑﯿﺎن ژن Klothoو ﺳﻄﺢ ﭘﺮوﺗﺌﯿﻦ ﻫﺎي Transforming growth factor-β ) TGF-β( و Platelet-derived growth ) PDGF factor( را در ﮐﺒﺪ ﻣﻮش ﻫﺎي ﭘﯿﺮ درﻣﺎن ﺷﺪه ﺑﺎ رژﯾﻢ ﻫﺎي ﻏﺬاﯾﯽ ﻣﺘﻔﺎوت )ﭘﺮ ﭼﺮﺑﯽ، ﭘﺮﭘﺮوﺗﺌﯿﻦ، ﮐﻢ ﮐﺎﻟﺮي، ﭘﺮﭘﺮوﺗﺌﯿﻦ- ﮐﻢ ﮐﺎﻟﺮي( ﻧﺸﺎن ﻣﯽ دﻫﺪ.
ﻣﻮاد و روشﻫﺎ: ﺑﯿﺴﺖ و ﭘﻨﺞ ﻋﺪد ﻣﻮش 20 ﻣﺎﻫﻪ ﻧﮋاد وﯾﺴﺘﺎر ﺑﻪ 5 ﮔﺮوه ﮐﻨﺘﺮل ﭘﯿﺮ )درﯾﺎﻓﺖ ﮐﻨﻨﺪه ﻏﺬاي ﻧﺮﻣﺎل(، ﮔﺮوه درﯾﺎﻓﺖ ﮐﻨﻨﺪه ﻏﺬاي ﭘﺮ ﭼﺮﺑﯽ، ﭘﺮﭘﺮوﺗﺌﯿﻦ، ﮐﻢ ﮐﺎﻟﺮي و ﭘﺮﭘﺮوﺗﺌﯿﻦ- ﮐﻢ ﮐﺎﻟﺮي ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ، ﻫﻤﭽﻨﯿﻦ ﯾﮏ ﮔﺮوه ﻣﻮش ﻫﺎي ﺟﻮان 6 ﻣﺎﻫﻪ ﻫﻢ ﺑﻪ ﻋﻨﻮان ﮔﺮوه ﮐﻨﺘﺮل ﺑﺎﻟﻎ در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﺪ. ﻣﻮش ﻫﺎ ﺑﻪ ﻣﺪت 10 ﻫﻔﺘﻪ ﺑﺎ رژﯾﻢ ﻫﺎي ﻏﺬاﯾﯽ درﻣﺎن ﺷﺪﻧﺪ. ﺳﻄﺢ ﺳﺮﻣﯽ ﭘﺮوﺗﺌﯿﻦ Klotho و ﺑﯿﺎن ژن Klotho ﺑﻪ وﺳﯿﻠﻪ روش Real-time PCR و ﺳﻄﺢ ﭘﺮوﺗﺌﯿﻦ ﻫﺎي TGF-β و PDGF در ﮐﺒﺪ ﺑﻪ وﺳﯿﻠﻪ ﮐﯿﺖ ﻫﺎي ﻣﺨﺼﻮص اﻻﯾﺰا اﻧﺪازه ﮔﯿﺮي ﺷﺪ.
ﯾﺎﻓﺘﻪﻫﺎ: ﻣﻘﺪار ﭘﺮوﺗﺌﯿﻦ Klotho در ﺳﺮم و ﺑﯿﺎن ژن آن در ﮐﺒﺪ ﻣﻮش ﻫﺎي درﻣﺎن ﺷﺪه ﺑﺎ رژﯾﻢ ﻏﺬاﯾﯽ ﭘﺮ ﭘﺮوﺗﺌﯿﻦ و رژﯾﻢ ﮐﻢ ﮐﺎﻟﺮي ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﻣﻮش ﻫﺎي ﭘﯿﺮ ﻧﺮﻣﺎل اﻓﺰاﯾﺶ ﭘﯿﺪا ﮐﺮده ﺑﻮد )0/000=P(. ﻣﯿﺰان ﻓﺎﮐﺘﻮرﻫﺎي ﮐﺒﺪي TGFβ و PDGF در رژﯾﻢ ﻏﺬاﯾﯽ ﭘﺮ ﭘﺮوﺗﺌﯿﻦ و رژﯾﻢ ﮐﻢ ﮐﺎﻟﺮي ﻧﺴﺒﺖ ﺑﻪ ﻣﻮش ﻫﺎي ﻧﺮﻣﺎل ﭘﯿﺮ اﻓﺰاﯾﺶ ﻣﻌﻨﯽ داري ﭘﯿﺪا ﮐﺮده اﺳﺖ )0/000=P(.
ﻧﺘﯿﺠﻪ ﮔﯿﺮي: ﺑﺮرﺳﯽ ﺣﺎﺿﺮ ﻧﺸﺎن داد ﮐﻪ رژﯾﻢ ﻫﺎي ﻏﺬاﯾﯽ ﻣﺨﺘﻠﻒ در اﻓﺮاد ﻣﺴﻦ ﺑﺴﯿﺎر ﻣﻬﻢ و ﺗﻌﯿﯿﻦ ﮐﻨﻨﺪه ﻣﯽ ﺑﺎﺷﺪ. ﺑﻪ ﻃﻮر ﮐﻠﯽ رژﯾﻢ ﮐﺎﻟﺮي ﻣﺤﺪود ﺑﺎ ﭘﺮوﺗﺌﯿﻦ زﯾﺎد اﺛﺮات ﻣﻔﯿﺪﺗﺮي ﻧﺴﺒﺖ ﺑﻪ رژﯾﻢ ﻫﺎي ﺑﺮرﺳﯽ ﺷﺪه دﯾﮕﺮ، ﺑﺮ روي ﻓﺎﮐﺘﻮر ﺿﺪ ﭘﯿﺮي دارد. ﻫﻤﭽﻨﯿﻦ اﯾﻦ رژﯾﻢ ﻫﺎ ﻣﯽ ﺗﻮاﻧﻨﺪ ﻋﻤﻠﮑﺮد ﮐﺒﺪ را ﺗﺤﺖ ﺗﺄﺛﯿﺮ ﻗﺮار داده و ﻓﺎﮐﺘﻮر ﻫﺎي ﺣﻔﺎﻇﺘﯽ را در آن زﯾﺎد ﮐﻨﻨﺪ. ﻣﻄﺎﻟﻌﺎت آﺗﯽ ﻧﯿﺎزﻣﻨﺪ اﺳﺘﻔﺎده از ﻣﻬﺎر ﮐﻨﻨﺪه ﻫﺎي ژن Klotho ﻣﯽ ﺑﺎﺷﺪ ﺗﺎ اﺛﺮات ﻣﺴﺘﻘﯿﻢ اﯾﻦ ژن را در ﭘﯿﺮي ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار دﻫﺪ
چكيده لاتين :
Different diets have important roles in human health and aging. In the current study, we
aimed to characterize the Klotho, an anti-aging protein, gene expression and protein level in the serum and assess the
protein levels of Transforming growth factor-β and Platelet-derived growth factor in old rats which treated with
different diets (high-fat, high-protein, low calorie, high-protein and low calorie).
Materials & Methods: Twenty-five old and five adult rats were treated with high-fat, high-protein, low calorie,
high-protein and low-calorie diets for ten weeks. Then, serum protein levels of Klotho, transforming growth factor-β
and platelet-derived growth factor and gene expression of Klotho in the liver were assessed using enzyme-linked
immunosorbent assay.
Results: Protein level of Klotho in the blood serum and expression level of Klotho gene in the liver wer e increased in
high-protein, low-calorie and low-calorie high-protein diets in comparison to control old group (p = 0.000). The levels
of transforming growth factor-β and platelet-derived growth factor were increased in low-calorie high-protein group,
compared to old group in the liver (p = 0.000).
Conclusion: Results revealed that expression of Klotho decreased in old rats while low-calorie high-protein and
particularly low-calorie high-protein diets increased this protein. Moreover, these protective diets could enhance
transforming growth factor-β and platelet-derived growth factor in liver of rats,veryfieing improvement of the liver
function.
عنوان نشريه :
علوم تغذيه و صنايع غذايي ايران
