به دليل نياز روزافزون به آگاهي بيش تر در مورد اثرات بازدارندگي گياهان، با توجه به نوع جغرافيا و تاثير آب و هوا بر روي آن ها، اثرات ضدباكتريايي اسانس چند گياه از خانواده لامياسه بر روي پنج باكتري گرم مثبت در مقايسه با سه آنتي بيوتيك بررسي شد.
مواد و روش ها
روش ديسك ديفيوژن با اندازه گيري قطر هاله بازدارنده در حجم 10، 15 و 20 ميكروليتر از اسانس هاي آويشن، مرزه، رزماري، پونه و نعناع با سه بار تكرار در مقايسه با سه آنتي بيوتيك پني سيلين و كلرامفنيكل، تتراسايكلين بر روي باسيلوس سريوس، استافيلوكوكوس اوريوس، انتروكوكوس فكاليس، انتروكوكوس فاسيوم و ليستريامونوسيتوژنز استفاده شد. تركيبات شيميايي اسانس ها با استفاده از روش گاز-كروماتوگرافي توسط شركت فروشنده آناليز شد (0/05
چكيده لاتين :
To investigate antibacterial activity of various volumes of five natural Lamiaceae plant
essential oils on five Gram-positive bacteria, compared to those of three antibiotics on these bacteria.
Materials & Methods: In this study, disk diffusion method was used and inhibition zone diameters of 10, 15 and 20
microliters of thyme, savory, rosemary, pennyroyal and spearmint essential oils against Bacillus cereus and
Enterococcus faecium, Listeria monocytogenes, Staphylococcus aureus and Enterococcus faecalis were recorded.
Chemical compositions of the essential oils were analyzed using gas chromatography.
Results: Inhibitory effects of the essential oils increased significantly with increasing their volumes, enhancing the
bacterial growth inhibition zone. Thyme and savory significantly decreased the bacterial growth, compared antibiotics.
Rosemary, mint and peppermint included smaller diameters of growth inhibition zones, compared to essential oils, with
no significant differences at 20 μl, compared to antibiotics.
Conclusion: Results have shown that thyme and savory include great inhibitory effects on growth rates of food
bacteria. In fact, 20 microliters of these herbals increase the shelf life of foods with great inhibitory effects. Comparison
of the effects of antibiotics and essential oils has demonstrated that savory includes the greatest inhibitory effect on
Enterococcus and thyme on Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus