بررسي تأثير سطوح مختلف اسيدآمينه ال- آرژنين بر شاخص‌هاي خون‌شناسي و مقاومت بچه‌ماهي كپور معمولي (Cyprinus carpio) در مقابل تنش شوري

Effects of different dietary levels of L-Arginine hematological parameters and salinity stress in fingerling common carp (Cyprinus carpio)

آرژنين يكي از اسيدهاي آمينه‌اي است كه تأثير آن بر بهبود رشد و سيستم ايمني انسان و بسياري از حيوانات، همواره مورد توجه پژوهشگران بوده است. در پژوهش حاضر تأثير اين اسيدآمينه بر فاكتورهاي خوني و مقاومت در برابر تنش شوري ماهي كپور معمولي مورد بررسي قرار گرفته است. به اين منظور ماهيان به چهار تيمار (هر تيمار با سه تكرار) تقسيم شدند و با جيره‌هاي غذايي شامل مقدار آرژنين 0، 5/1، 2 و 5/2 درصد، به‌مدت 60 روز تغذيه شدند. در نهايت نمونه‌هايي از هر تيمار خون‌گيري و ساير ماهي‌ها تحت تنش شوري ppt 15 به‌مدت 72 ساعت قرار گرفتند. تجزيه ‌و تحليل داده‌ها با مقايسه ميانگين تيمارها در قالب طرح كاملاً تصادفي به كمك آزمون دانكن صورت گرفت. بر طبق نتايج خون‌شناسي، تغذيه ماهي كپور معمولي توسط آرژنين بر تعداد گلبول‌هاي قرمز، هموگلوبين و شاخص‌هاي MCV، MCH و MCHC خون آن‌ها تأثير معناداري داشت (05/0>P) ولي تعداد گلبول‌هاي سفيد و هماتوكريت در تيمارهاي مختلف اختلاف معناداري نداشت (05/0

Arginine is one of the amino acids that its impact on improving the growth and immune system of human and many animals always been one of interest of researchers. In this study, the effect of this amino acid on growth indices, blood factors and resistance against salinity tension on common carp fish have been investigated. For this purpose, four treatments (each treatment with three replicates) were made from common carp and fed with dietary foods containing arginine 0, 1.5, 2 and 2.5% for 60 days. During this period, the fishes three times were biometrics; finally, samples of blood of each treatment have been taken and other fish were subjected to salinity stress (ppt 15) for 72 hours. Data analysis was performed using SPSS software version 18 and Excel and the comparing the mean of treatments in a completely randomized design have been performed analysis with Duncan test and one way ANOVA and existent or lack significant difference were performed in 5% of the level Probability (P<0.05). Also, according to Haematology results, feeding of common carp fish by arginine had a significant effect on the size of red blood cells, hemoglobin and MCH, MCV and MCHC blood indices (P<0.05), but the number of white blood cells and hematocrit in different treatments were not significantly different (P>0.05). Results of applying of salinity stress on different treatments showed that the presence of arginine in the feed of carp fish may have a significant effect on their resistance and survival, so that for feed treatments with 1.5% and 2% of arginine within 72 hours did not show any casualties, therefore, based on the obtained results, arginine-based amino acids, have a significant effect on body immunity and survival of carp fish.