از طريق كشت درون شيشه اي ULMUS GLABRA تكثير ملچ

Micropropagation of mature Ulmus glabra

Ulmus glabra, is the most endangered species of northern forests of Iran, due to the Dutch elm disease; older trees with high diameters is quite rare in northern forests today. These experiments were carried out as a step toward clonal propagation of superior genotypes. The effects of various hormone concentrations were investigated on the buds or three genotypes of Ulmus glabra. One of the chosen trees was juvenile and the two other were adult trees. Axillary and apical buds were collected in winter and cultured on DKW medium supplemented with different concentrations of BA. (0.02, 0.5 1, 2 mg/lit) and IBA (0,2, 0.1 mg/lit). Immersing buds in 70% (DKW. ethanol for five minutes and subsequently application of 3%. DKW. Hypochloride forests minutes. Was the best way to remove surface contamination. Complete removed bud scales was necessary. There was always some callus formarion at the base of the micro shoots. Higher concentrations of BA. caused more callus fromation. The best medium for shoot proliferation was DKW supplemented with 1mg/l IBA and 0.01 mg/l IBA. Shoots of different genotype rooted very easily. The juvenile genotype showed the highest rate of shoot proliferation and lowest amount of callus formation. Different genotypes showed different rates of rooting. All of the transferred plantlers established well in the field.