به ژنوم آرابيدوپسيس T-DNA بررسي نحوه توارث عوامل مقاومت در برابر يونيكانازل در جهش يافته هاي حاصل از ورود

Inheritance of uniconazol resistance factors in T-DNA inserted mutant genotypes of Arabidopsis thaliana

Arabidopsis thaliana is a favourite model plant for biotechnology studies by which a remarkable development happened in plant sciences including plant breeding during the last decade. Inducing useful mutants, resistant to various environmental stresses, their recognition and transferring to other plant species, lacking the resistance, is one of the major application of the species. Resistance to herbicides is one of the characteristics, which is very attractive to the breeders. This research was carried out to investigate the inheritance mode of the factors controlling resistance to uniconazol (a GA-biosynthesis inhibitor). Forty-eight variable gene pools created through inserting T-DNA to the genome of the Colombia ecotype of the species were received from Prof. Cutler from Toronto university, Canada, and used in this research to identify the uniconazol resistance genotypes. Twelve concentrations of the herbicide were tested on the Colombia ecotype to find the critical dilution of uniconazol on which no wild type seed can germinate and grow. The gene pools were screened on the critical dilution and a number of the resistant seedlings were recognized. To investigate their gene function and the number of genes controlling the character, one of the mutants was crossed to the wild type Colombia and Landsberg ecotypes. F1 and F2 generations were also generated. Testing the parents and the two F1 and F2 generations on the critical dilution of the chemical, revealed that one dominant gene is controlling the character in the mutant.