بررسي استفاده از پسماند هاي مايع عرقيات بوقناق، بهارنارنج و رازيانه به عنوان يك منبع بالقوه آنتي اكسيداني و ضد باكتريايي

Survey on the use of Eryngium billardieri, Citrus aurantium and Foeniculum vulgare distillate wastewaters as a potential source antioxidant and antibacterial

ﺳﺎﺑﻘﻪ و ﻫﺪف: ﭘﺴﻤﺎﻧﺪﻫﺎي ﮐﺎرﺧﺎﻧﺠﺎت ﻋﺮﻗﯿﺎت ﮔﯿﺎﻫﯽ ﺑﻪﻋﻨﻮان ﯾﮏ ﻣﻨﺒﻊ ﺗﺮﮐﯿﺒﺎت آﻟﯽ ﻣﯽﺑﺎﺷﻨﺪ. ﺑﻨﺎﺑﺮاﯾﻦ ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﯽ اﺛﺮات ﺿﺪﺑﺎﮐﺘﺮﯾﺎﯾﯽ و آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﭘﺴﻤﺎﻧﺪﻫﺎي ﺻﻨﻌﺘﯽ ﻋﺮﻗﯿﺎت ﺑﻮﻗﻨﺎق، ﺑﻬﺎرﻧﺎرﻧﺞ و رازﯾﺎﻧﻪ در ﺷﺮاﯾﻂ آزﻣﺎﯾﺸﮕﺎﻫﯽ ﺑﻮد. ﻣﻮاد و روشﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ، ﭘﺴﻤﺎﻧﺪﻫﺎي ﻋﺮﻗﯿﺎت ﺑﻮﻗﻨﺎق، ﺑﻬﺎرﻧﺎرﻧﺞ و رازﯾﺎﻧﻪ از ﯾﮏ ﮐﺎرﺧﺎﻧﻪ ﺗﻮﻟﯿﺪي در ﮐﺎﺷﺎن ﺟﻤﻊآوري ﺷﺪ. ﭘﺲ از ﻓﯿﻠﺘﺮﮐﺮدن، ﻣﺤﻠﻮلﻫﺎي ﺑﻪدﺳﺖآﻣﺪه، ﺧﺸﮏ و ﻣﯿﺰان ﺗﺮﮐﯿﺒﺎت ﻓﻨﻮﻟﯽ و ﻓﻼوﻧﻮﺋﯿﺪي ﺑﻪ روش رﻧﮓﺳﻨﺠﯽ ﺷﻨﺎﺳﺎﯾﯽ ﺷﺪﻧﺪ. ﻫﻤﭽﻨﯿﻦ ﺧﺎﺻﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﺑﻪ روش اﺣﯿﺎي آﻫﻦ و ﺧﺎﺻﯿﺖ ﺿﺪﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻋﻠﯿﻪ اﺳﺘﺎﻓﯿﻠﻮﮐﻮﮐﻮس اورﺋﻮس و اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ ﻣﻘﺎوم ﺑﻪ آﻧﺘﯽﺑﯿﻮﺗﯿﮏ ﺑﻪ روش ﻣﯿﮑﺮوداﯾﻠﻮﺷﻦ و دﯾﺴﮏ دﯾﻔﻮژن ﺑﺮرﺳﯽ ﺷﺪﻧﺪ. ﻧﺘﺎﯾﺞ: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان ﺗﺮﮐﯿﺒﺎت ﻓﻨﻮﻟﯽ )109/46 ﻣﯿﻠﯽﮔﺮم در ﮔﺮم( و ﻓﻼوﻧﻮﺋﯿﺪي )13/6 ﻣﯿﻠﯽﮔﺮم در ﮔﺮم( در ﭘﺴﻤﺎﻧﺪ ﻋﺮق رازﯾﺎﻧﻪ ﺑﻮد. ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان ﺧﺎﺻﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﻧﯿﺰ ﺑﻪﺗﺮﺗﯿﺐ ﺑﺮاي رازﯾﺎﻧﻪ 1229/9±30/4، ﺑﻬﺎرﻧﺎرﻧﺞ 678/39±1/3 و ﺑﻮﻗﻨﺎق 2 549/15±28/3 ﻣﯿﻠﯽﻣﻮل +Fe در ﮔﺮم در ﺑﺎﻻﺗﺮﯾﻦ ﻏﻠﻈﺖ آنﻫﺎ ﺑﻮد. ﻫﯿﭻﮐﺪام از ﭘﺴﻤﺎﻧﺪﻫﺎ ﺑﺮ اﺳﺘﺎﻓﯿﻠﻮﮐﻮﮐﻮس اورﺋﻮس اﺛﺮ ﻧﺪاﺷﺘﻨﺪ، وﻟﯽ رازﯾﺎﻧﻪ و ﺑﻮﻗﻨﺎق ﺑﺎ ﻣﯿﺰان MIC )166/66 ﻣﯿﻠﯽﮔﺮم در ﻣﯿﻠﯽﻟﯿﺘﺮ( و MBC )333/33 ﻣﯿﻠﯽﮔﺮم در ﻣﯿﻠﯽﻟﯿﺘﺮ( اﺛﺮ ﺿﺪ اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ ﻧﺸﺎن دادﻧﺪ. ﻧﺘﯿﺠﻪﮔﯿﺮي: اﯾﻦ ﻣﻄﺎﻟﻌﻪ اوّﻟﯿﻦ ﺑﺮرﺳﯽ در زﻣﯿﻨﻪ ﻓﻌﺎﻟﯿﺖ ﺑﯿﻮﻟﻮژﯾﮑﯽ ﭘﺴﻤﺎﻧﺪﻫﺎي ﻋﺮﻗﯿﺎت ﮔﯿﺎﻫﯽ در اﯾﺮان ﺑﻮد و ﻧﺘﺎﯾﺞ ﺣﺎﮐﯽ از وﺟﻮد ﺗﺮﮐﯿﺒﺎت ﻓﻌﺎل و اﺛﺮات ﺑﯿﻮﻟﻮژﯾﮑﯽ اﯾﻦ ﭘﺴﻤﺎﻧﺪﻫﺎ ﺑﻮد. ﭘﯿﺸﻨﻬﺎد ﻣﯽﺷﻮد ﻣﻄﺎﻟﻌﺎت ﮔﺴﺘﺮدهﺗﺮي در ﺧﺼﻮص وﯾﮋﮔﯽﻫﺎي ﺿﺪﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻋﻠﯿﻪ اﻧﻮاع ﺑﺎﮐﺘﺮيﻫﺎي ﮔﺮم ﻣﻨﻔﯽ و ﻣﺜﺒﺖ، ﻫﻤﭽﻨﯿﻦ ﺷﻨﺎﺳﺎﯾﯽ ﺳﺎﯾﺮ ﺗﺮﮐﯿﺒﺎت و اﺛﺮات ﺑﯿﻮﻟﻮژﯾﮑﯽ ﻣﺎﻧﻨﺪ ﺳﻤﯿﺖ ﺳﻠﻮﻟﯽ اﯾﻦ ﭘﺴﻤﺎﻧﺪﻫﺎ ﺻﻮرت ﮔﯿﺮد.

Waste of plant distillates industries are a source of organic compounds. Therefore, this study aimed to evaluate the antibacterial and antioxidant effects of Eryngium billardieri, Citrus aurantium and Foeniculum vulgare wastewaters materials in vitro. Materials and Methods: In this experimental study, samples of Eryngium billardieri, Citrus aurantium and Foeniculum vulgare wastewaters were collected at one of the factories in Kashan. After filtration and drying of the obtained solutions, total phenolic and flavonoid compounds were determined by colorimetric method. Also, antioxidant activity was evaluated by ferric reducing ability and antibacterial activities against antibiotic resistance Staphylococcus aureus and Escherichia coli using the micro-dilution and disc diffusion methods. Results: The results showed that the highest amount of phenolic compounds (106. 46 mg/g) and flavonoids (13.6 mg/g) were in the F. vulgare wastewaters. The highest antioxidant activity was for F. vulgare (1229.9±30.44), C. aurantium (679.39±1.3), and E. billardieri (549.28±15.35 mmol Fe 2+/g at their highest concentration, respectively. None of the wastewaters samples had any effect on S. aureus, but F. vulgare and E. billardieri showed an anti-E. coli effect with MIC (166.66 mg/mL) and MBC (333.3 mg/mL). Conclusion: This study was the first research on the biological activity of herbal distillate wastewaters in Iran. The results showed the presence of active compounds and the biological effects of these wastes. It is suggested that more extensive studies be carried out on the antibacterial activities on various gram negative and positive bacteria, as well as the identification of other compounds and biological properties such as cytotoxicity of herbal distillate wastewaters