تاثير تمرين تناوبي شديد (HIIT) بر بيان ژن هاي MMP-2 ، COL-III و عملكرد قلبي ميوكارد موش هاي صحرايي مبتلا به ديابت نوع2

Effect of high intensity interval training (HIIT) on the ge ne expression of MMP-2, COL-III and myocardial function in type 2 diabetic rats

تمرينات ورزشي منظم با حجم متفاوت در سطح گلوكز خون نوسان ايجاد كرده، بيان ژن را تعديل مي كند و هدف از اين مطالعه تعيين تاثير تمرين تناوبي شديد (HIIT) بر بيان ژن هاي MMP-2 ، COL-III و عملكرد قلبي ميوكارد موش هاي صحرايي مبتلا به ديابت نوع2 بود. مواد و روش ها پژوهش حاضر از نوع تجربي است. بدين منظور 16 سر موش نر ديابتي به دو گروه 8 تايي; تمرين تناوبي شديد (HIIT) و كنترل (C) تقسيم شدند . القاء ديابت به صورت پلت با رژيم غذايي پرچرب (30 درصد چربي و 25 درصد فروكتوز) به مدت 24 هفته انجام شد. پس از آخرين جلسه تمرين و ريكاوري بعد از آن، آزمودني ها قرباني و بطن چپ آن ها استخراج شد. جهت تعيين بيان ژن هاي MMP-2 و COL-III از روش PCRReal time- و براي سنجش عملكرد قلبي از M-mode اكوكارديوگرافي استفاده شد. جهت مقايسه گروه ها از آزمون t مستقل در سطح آلفاي 0/05 استفاده شد. نتيجه گيري به نظر مي رسد كه احتمالا تمرين HIIT مي تواند با كاهش بيان ژن هاي MMP-2 و COL-III كارديوميوپاتي ديابتي را كاهش داده و عملكرد قلب را بهبود بخشد.

Regular exercise training the blood glucouse level fluctuates, modulates gene expression and reduse diabetic cardiomyopathy. The purpose of this study was to determine the effect of High intensity Interval training (HIIT) on the gene expression of MMP2- , COL-III and myocardial function in type 2 diabetic rats. Materials and Methods: This is an experimental study. For this purpose, 16 male diabetic rats were divided into 2 groups of numbers 8; High intensity Interval training (HIIT) and Control (C). Diabetes was induced in a pellet with a high-fat diet (%30 fat and %25 fructose) for 24 weeks. After the last training session and afterwards recovery, subjects were sacrificed and their left ventricle was extracted. PCR-Real time was used to determine the expression of MMP2- and COL-III genes and for the assessment of cardiac function was used M- mode echocardiography. to compare the groups independent t-test was used at alpha level of 05/0. Results: genes expression of MMP2- and COL-III in the training group respectively showed a significant decrease compared to the control group (P= 0.001) and (p=0.02). Weight did not significantly change in any of the groups. Glucose, insulin and insulin resistance index in the training group showed a significantly change compared to the control group.Ejection fraction(EF) and shortening fraction(SF) in training group respectively were sinificantly increase compared to the control group(P=0.001) and (P=0.01). Conclusion: It seems that,HIIT training possibely can with decreases the expression of MMP2- and COL-III genes in diabetic cardiomyopathy and improves cardiac function.