بررسي تاثيركاربرد ليزركم توان بر ترميم نقص استخواني در موش هاي صحرايي ماده ديابتيك و استئوپوروتيك با استفاده از تكنيك Real-time PCR

Effect of Low-level Laser Therapy on Bone Defect Repairin Diabetic and Osteoporotic Rats using the Real-Time PCR Technique

در مبتلايان به ديابت نوع يك تشكيل استخوان مختل شده و ريز ساختار استخوان تغيير مي كند. مهم ترين عارضه متابوليكي در ديابت، استيوپوروز بوده كه با كاهش توده استخواني و تخريب ساختار استخوان مشخص مي شود. هدف از تحقيق حاضر تعيين تاثير كاربرد ليزر كم توان در ترميم نقص استخواني در مدل تجربي ديابت و استيوپوروز مي باشد. ﻣﻮاد و روش ﻫﺎ: 30 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﻣﺎده ﭼﻬﺎر ﻣﺎﻫﻪ، ﻧﮋاد وﯾﺴﺘﺎر ﺑﺎ وزن 190-220 ﮔﺮم اﻧﺘﺨﺎب و ﺑﻪ ﻃﻮر ﺗﺼﺎدﻓﯽ ﺑﻪ ﺷﺶ ﮔﺮوه ﺷﺎﻣﻞ 1: ﮐﻨﺘﺮل ﻏﯿﺮ دﯾﺎﺑﺘﯽ).Co (، 2: ﻟﯿﺰر ﻏﯿﺮ دﯾﺎﺑﺘﯽ).L(، 3: ﮐﻨﺘﺮل دﯾﺎﺑﺘﯽ).Co.D(، 4: ﻟﯿﺰردﯾﺎﺑﺘﯽ).L.D(، 5: آﻟﻨﺪروﻧﯿﺖ دﯾﺎﺑﺘﯽ).A.D(، و 6: ﻟﯿﺰر+آﻟﻨﺪروﻧﯿﺖ دﯾﺎﺑﺘﯽ).L.A.D( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. دﯾﺎﺑﺖ در ﮔﺮوه ﻫﺎي 5 ،4 ،3 و 6 اﻟﻘﺎ ﺷﺪ. ﻫﻤﻪ ﮔﺮوه ﻫﺎ ﺗﺤﺖ ﻋﻤﻞ ﺟﺮاﺣﯽ اوارﮐﺘﻮﻣﯽ و ﻧﻘﺺ اﺳﺘﺨﻮاﻧﯽ ﺟﺰﺋﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. در ﮔﺮوه ﻟﯿﺰر، ﻟﯿﺰر ﮐﻢ ﺗﻮان) nm, 1/5 J/cm2 , 80 Hz 890( ﺑﻪ 3 ﻧﻘﻄﻪ در ﻣﺤﻞ ﻧﻘﺺ ﺗﺎﺑﺎﻧﺪه ﺷﺪ. ﺑﻌﺪ از ﯾﮏ ﻣﺎه، اﺳﺘﺨﻮان ﻫﺎي ﺗﯿﺒﯿﺎ ﺑﺮداﺷﺖ و -Real time PCR اﻧﺠﺎم ﺷﺪ. داده ﻫﺎي ﮔﺮوه ﻫﺎ ﺑﺎ روش ANOVA ﺑﺎ ﺳﻄﺢ ﻣﻌﻨﯽ داري)0.05

Bone formation is disturbed in type 1 diabetes followed by changes in the bone microstructure. The most important metabolic disorder in diabetes is osteoporosis, which is characterized by bone loss and bone structure degradation. This study aimed to determine the effect of low-power laser on bone defect repair in the experimental model of diabetes and osteoporosis. Materials & Methods A total of 30 four-month-old female Wistar rats weighing 190-220 g were selected and randomly divided into six groups, including 1: non-diabetic control (Co.), 2: non-diabetic laser (L.), 3: diabetic control (Co.D.), 4: diabetic laser (L.D.), 5: diabetic alendronate (A.D.), and 6: diabetic laser + alendronate (L.A.D.). Diabetes was induced in groups 3, 4, 5, and 6. All groups underwent ovariectomy and partial bone defect. In the laser group, a low-level laser (890nm, 80 Hz, 1/5J / cm 2) was radiated to 3 points at the defect location. Tibia bones were collected, and Real-time PCR was performed after a month. The data were analyzed using ANOVA. A p-value less than P<0.05 was considered statistically significant. Ethics code: 13237-91-1-1393-10397 Findings The t-test showed a significant decrease in tibia bone density in diabetic and osteoporotic rats, compared to the non-diabetic control group. Moreover, analysis of gene expression data (ANOVA, P<0.05) revealed a significant difference between the group of diabetic laser + alendronate and other groups in terms of Runx2 gene expression and Osteocalcin. Discussions & Conclusions According to the findings, laser therapy combined with alendronate can accelerate the repair of partial bone defect in the experimental model of diabetes and osteoporosis.