ارزيابي اثرات آنتي باكتريال اسپري ضدعفوني كننده سريع الاثر و فاقد الكل (Virobac Spray)

Evaluation of Antibacterial Activity of Non-alcoholic Quick Spray (Virobac Spray)

اﻣﺮوزه ﺑﺮاي ﮐﻨﺘﺮل ﻋﻔﻮﻧﺖ در ﺑﯿﻤﺎرﺳﺘﺎنﻫﺎ ﺿﺪ ﻋﻔﻮﻧﯽ ﺳﺮﯾﻊ و ﮐﺎﻣﻞ ﺳﻄﻮح ﻣﺤﯿﻄﯽ و ﺗﺠﻬﯿﺰاﺗﯽ از ﺿﺮورﯾﺎت اﺳﺖ. ازﺟﻤﻠﻪ اﯾﻦ ﻣﻮارد ﻣﯽﺗﻮان ﺑﻪ ﺗﺠﻬﯿﺰات ﭘﺰﺷﮑﯽ و دﻧﺪاﻧﭙﺰﺷﮑﯽ ﺣﺴﺎس ﺑﻪ اﻟﮑﻞ، ﺳﻄﻮح ﻣﺤﯿﻄﯽ ﺑﯿﻤﺎرﺳﺘﺎﻧﯽ، آزﻣﺎﯾﺸﮕﺎهﻫﺎ و ﮐﻠﯿﻨﯿﮏﻫﺎي ﺳﺮﭘﺎﯾﯽ اﺷﺎره ﮐﺮد. ﻫﺪف از ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ﺑﺮرﺳﯽ و ﺗﻌﯿﯿﻦ ﮐﯿﻔﯿﺖ اﺳﭙﺮي ﺿﺪﻋﻔﻮﻧﯽ ﮐﻨﻨﺪه ﺳﺮﯾﻊ اﻻﺛﺮ و ﻓﺎﻗﺪ اﻟﮑﻞ Virobac Spray)( ﺗﻮﻟﯿﺪ اﯾﺮان و ﺗﺎﺛﯿﺮ ﻓﺎﮐﺘﻮر رﻗﺖ ﺑﺮ زﻣﺎن اﻋﻤﺎل اﺛﺮ ﺿﺪﻣﯿﮑﺮوﺑﯽ ﺑﻮده اﺳﺖ. روش ﮐﺎر اﺛﺮﺿﺪﻣﯿﮑﺮوﺑﯽ اﺳﭙﺮي ﺿﺪﻋﻔﻮﻧﯽ ﮐﻨﻨﺪه ﺳﺮﯾﻊ اﻻﺛﺮ و ﻓﺎﻗﺪ اﻟﮑﻞ Virobac Spray)( ﺑﺮ ﭼﻬﺎر ﮔﻮﻧﻪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﮔﺮم ﻣﺜﺒﺖ و ﻣﻨﻔﯽ ارزﯾﺎﺑﯽ ﺷﺪ ﮐﻪ ﺷﺎﻣﻞ: اﺳﺘﺎﻓﯿﻠﻮﮐﻮﮐﻮس اورﺋﻮس)29737 ATCC (، ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﯾﻨﻮزا )28753 ATCC (، اﺷﺮﺷﯿﺎﮐﻠﯽ )25922 ATCC ( و اﻧﺘﺮوﮐﻮﮐﻮس ﻓﮑﺎﻟﯿﺲ )1237 ATCC( ﺑﻮد. در اداﻣﻪ ﺑﺮرﺳﯽ اﺛﺮ ﺿﺪﺑﺎﮐﺘﺮﯾﺎﯾﯽ در رﻗﺖﻫﺎي 1/2 1/4 ، و 1/8 از اﺳﭙﺮي ﺿﺪﻋﻔﻮﻧﯽ ﮐﻨﻨﺪه ﺳﺮﯾﻊ اﻻﺛﺮ و ﻓﺎﻗﺪ اﻟﮑﻞ Virobac Spray)( ﺻﻮرت ﮔﺮﻓﺖ و ﻣﯿﺰان اﺛﺮ ﮔﺬاري و ﻧﺎﺑﻮدي ﺑﺎﮐﺘﺮيﻫﺎ ﺑﻌﺪ از زﻣﺎنﻫﺎي ﻣﺠﺎورت45 و 60 و90 و120 ﺛﺎﻧﯿﻪ ﺗﻌﯿﯿﻦ ﮔﺮدﯾﺪ. ﻧﺘﺎﯾﺞ اﺳﭙﺮي ﺿﺪﻋﻔﻮﻧﯽ ﮐﻨﻨﺪه ﺳﺮﯾﻊ اﻻﺛﺮ و ﻓﺎﻗﺪ اﻟﮑﻞ Virobac Spray)(در ﻏﻠﻈﺖ آﻣﺎده ﻣﺼﺮف و ﺣﺘﯽ رﻗﺖ ﯾﮏ دوم در ﺗﻤﺎﻣﯽزﻣﺎنﻫﺎي ﻣﺠﺎورت، اﺛﺮ ﺿﺪﻣﯿﮑﺮوﺑﯽ ﻣﻨﺎﺳﺒﯽ روي ﻫﺮ ﭼﻬﺎر ﮔﻮﻧﻪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻣﻮرد ﻣﻄﺎﻟﻌﻪ داﺷﺖ. اﯾﻦ در ﺣﺎﻟﯽ اﺳﺖ ﮐﻪ ﺣﺘﯽ در آزﻣﺎﯾﺶ ﺑﺎ رﻗﺖﻫﺎي ﮐﻤﺘﺮ از ﯾﮏ ﭼﻬﺎرم ﻧﯿﺰ ﻗﺪري اﺛﺮ ﻣﻬﺎري ﺑﺮ روي ﭘﺴﻮدوﻣﻮﻧﺎس آﺋﺮوژﯾﻨﻮزا و اﺷﺮﺷﯿﺎﮐﻠﯽ دﯾﺪه ﺷﺪ و ﺑﻪ وﯾﮋه در زﻣﺎنﻫﺎي ﺑﺎﻻﺗﺮ اﺛﺮ ﻣﻬﺎري ﻗﺎﺑﻞ ﻗﺒﻮﻟﯽ ﺑﺮ روي اﺳﺘﺎﻓﻠﻮﮐﻮﮐﻮس اورﺋﻮس و اﻧﺘﺮوﮐﻮﮐﻮس ﻓﮑﺎﻟﯿﺲ ﻧﺸﺎن داد. ﻧﺘﯿﺠﻪ ﮔﯿﺮي اﺳﭙﺮي ﺿﺪﻋﻔﻮﻧﯽ ﮐﻨﻨﺪه ﺳﺮﯾﻊ اﻻﺛﺮ و ﻓﺎﻗﺪ اﻟﮑﻞ Virobac Spray)(ﻃﯿﻒ اﺛﺮ وﺳﯿﻌﯽ ﺑﺮ روي ﺑﺎﮐﺘﺮيﻫﺎي ﻣﻘﺎوم ﺑﯿﻤﺎرﺳﺘﺎﻧﯽ دارد و در ﺗﻤﺎم زﻣﺎﻧﻬﺎي ﻣﺠﺎورت ﻣﻮﺟﺐ ﺣﺬف ﮐﺎﻣﻞ اﺷﺮﺷﯿﺎﮐﻠﯽ اﻧﺘﺮوﮐﻮﮐﻮس ﻓﮑﺎﻟﯿﺲ، ﭘﺴﻮدوﻣﻮﻧﺎس آﺋﺮوژﯾﻨﻮزا و اﺳﺘﺎﻓﯿﻠﻮﮐﻮﮐﻮس اورﺋﻮس ﻣﯽﺷﻮد.

Introduction: Now a day, there is a crucial need for a quick and effective disinfectant to prevent nosocomial infections in hospitals. Meanwhile evaluation and validation of disinfectants are important. hospital surfaces, instruments, and rooms. the aim of this study was to evaluate the effect, quality and disinfectant activity of the Quick spray (Virobac Spray). Material and methods: Antibacterial activity of Quick spray (Virobac Spray) was evaluated on four different gram-positive and gram-negative bacterial strain including Staphylococcus aureus (ATCC 29737), Pseudomonas aeruginosa (ATCC 28753), Escherichia coli (ATCC 25922) and Enterococcus faecalis (ATCC 1237. The antimicrobial solution was examined in ½, ¼, and 1/8 dilutions, and the antibacterial effect was determined after the exposure time such as 45, 60, 90, and 120 seconds. Result: Quick spray (Virobac Spray) show a powerful antibacterial activity even in ½ dilution, also we demonstrate that the antibacterial activity of this solution will be enhanced with increased time of exposure. Conclusion: the result of this study demonstrated that Quick spray (Virobac Spray) is a broad-spectrum and effective disinfectant and are able to inhibit the growth of a wide range of bacteria including gram-positive and gram-negative bacteria