مطالعه لكتين هيستوشيمي قندها انتهايي مختلف سطح سلول هاي غده آدرنال در طول تكامل موش

Lectinhistochemical studies of different terminal sugars of adrenal cells surface during mouse embryogenesis

Background: Changes in cell surface associated glycoconjugates are known to affect cell differentiation , cellular interactions and other developmental phenomena during embryogenesis. glycoconjugates, particularly their sugar side chains, play important roles in embryonic development. This study was conducted to examine Chemical nature of glycoconjugate Components on the surface of developing mouse medullary Cells of the Suprarenal gland. Material and Methods: 212 samples of murine embryogenes and newborns at days 1-15 was fixed in B4G and Formalin, then Processed for lectin histochemistry. 5 micrometer paraffin sections were incubated with 10-15 pglml Solution of HRP/lectins from Vicia villosa Agglutinin (VVA) ,Ornge Fungus Agglutinin (OFA) , Lotus Tetragonolobus Agglutinin(LTA) , Ulex Europeus Agglutinin 1 (UEAI) and Griffonia Simplicifolia Agglutinin 1-B4(GSA1-B4) in 0.1 M PBS (Phosphat Buffer Solution), pH 7.2. All sections were developed in Substrate medium Containing 0.03% DAB (Di Amino Benzidin) and 0.006% H202 in PBS buffer pH 7.2. Results:From five the tested lectins, just OFA , LTA and UEA1 had reaction with developing medullary cells of the suprarenal glands. Conclusion: The timing and distribution of staining with the lectins Orange Fungus Agglutinin(OFA) , Lotus Tetragonolobus Agglutinin(LTA)andUlex Europeus Agglutininl(UEA1) suggest that this fucosylated glycoconjugate may play a role in directing the migration of neural crest cells in to the suprarenal medulla and subsequent formation of the chromaffin cells .