VOLTAGE CLAMP اثرات تركيب جديد كوييناز و لينوني بر جريانهاي يوني القا شده در يك مدل صرعي با استفاده از تكنيك

Background: Epilepsy is a neurological disorder in which nerve cells of the brain from time to time release abnormal electrical impulses. These cause a temporary malfunction of the never cells of the brain. Materials and methods: In this experimental study, two electrode voltage and current clamp techniques were used to determine the efficacy of a new quinazolinone compound on PTZ inducted epileptiform activity and underlying ionic conductance in D5 neuronal membrane of Helix aspersa. Results: The results demonstrated that the convulsant drug PTZ ( 25 mM ) changed the shape and increased the frequency of spontaneous action potentials. At holding potential of -- 40 mV , PTZ led to a reduction in amplitude of inward and outward currents. Extracellular application of the new quinazolinone compound (0.1 mM) in epileptic model produced a hyperpolarization of the resting membrane potential and a decrease in frequency of action potentials. Under voltage clamp condition, tested new compound decreased the peak amplitude of the inward and increased the outward currents. Application of the new compound added PTZ , caused a reduction in frequency , amplitude and duration potential. Resting membrane potential was affected and shifted towards more hyperpolarized voltage. At holding potential of -- 40 mV , the new compound in the absence of PTZ depressed the total membrane conductance. Simultaneous application of quinazolinone and PTZ produced a remarkable reduction in membrane ionic currents. Conclusion: It may be concluded that new quinazolinone compound causes a reduction in cellular excitability and inhibition of PTZ induced epileptic activity via changing membrane ionic conductance .