عنوان مقاله :
بررسي فعاليت ضد باكتريايي لاكتوباسيلوس پلانتاروم و لاكتوباسيلوس كازيي بر باكتري بورخولدريا جدا شده از بيماران بستري شده در بيمارستان امام علي (ع) شهرستان آمل
عنوان به زبان ديگر :
Antibacterial Activity of Lactobacillus plantarum and Lactobacillus casei on Burkholderia cepacia Isolated from Imam Ali Hospital
پديد آورندگان :
صادقي، مليحه دانشگاه آيت الله آملي، آمل، ايران , ايزدي آملي، رابعه دانشگاه آيت الله آملي - گروه زيست شناسي، آمل، ايران , فتاحي، اسماعيل دانشگاه آيت الله آملي - گروه زيست شناسي، آمل، ايران
كليدواژه :
پروبيوتيك , لاكتوباسيلوس پلانتاروم , لاكتوباسيلوس كازيي , بورخولدريا سپاشيا , عفونت‌هاي بيمارستاني
چكيده فارسي :
زﻣﻴﻨﻪ و ﻫﺪف: ﻋﻔﻮﻧﺖﻫﺎي ﺑﻴﻤﺎرﺳﺘﺎﻧﻲ از ﻋﻠﻞ ﺷﺎﻳﻊ ﻣﺮگ و ﻣﻴﺮ، اﻓﺰاﻳﺶ ﻃﻮل ﻣﺪت ﺑﺴﺘﺮي و اﻓﺰاﻳﺶ ﻫﺰﻳﻨﻪﻫﺎي ﺑﻴﻤﺎرﺳﺘﺎﻧﻲ ﻣﻲﺑﺎﺷﻨﺪ. اﻣﺮوزه اﺳﺘﻔﺎده از ﺑﺎﻛﺘﺮيﻫﺎي ﭘﺮوﺑﻴﻮﺗﻴﻚ، ﺑﻪ ﻋﻨﻮان ﻣﻬﺎرﻛﻨﻨﺪه و ﺿﺪ ﺑﺎﻛﺘﺮيﻫﺎ، ﻣﻮرد ﺗﻮﺟﻪ ﻗﺮارﮔﺮﻓﺘﻪ اﺳﺖ. ﻫﺪف از اﻳﻦ ﻣﻄﺎﻟﻌﻪ، ﺗﻌﻴﻴﻦ ﻓﻌﺎﻟﻴﺖ ﺿﺪﻣﻴﻜﺮوﺑﻲ ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﭘﻼﻧﺘﺎروم و ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﻛـﺎزﻳﻲ در ﻣﻘﺎﺑـﻞ ﺑﻮرﺧﻮﻟـﺪرﻳﺎ ﺳﭙﺎﺷـﻴﺎ ﺟﺪاﺷﺪه از ﺑﻴﻤﺎرﺳﺘﺎن اﻣﺎم ﻋﻠﻲ )ع( ﺷﻬﺮﺳﺘﺎن آﻣﻞ ﺑﻮده اﺳﺖ.
ﻣﻮاد و روش ﻫﺎ: دراﻳﻦ ﺗﺤﻘﻴﻖ، ﺟﺪاﺳﺎزي ﺑﺎﻛﺘﺮي ﺑﻮرﺧﻮﻟﺪرﻳﺎ ﺳﭙﺎﺷﻴﺎ از ﺑﻴﻤـﺎران ﺑﻴﻤﺎرﺳـﺘﺎن اﻣـﺎم ﻋﻠـﻲ ع( آﻣـﻞ ﺑـﺎ روش ﻛﺸﺖ، ﺗﺴﺖﻫﺎي ﺑﻴﻮﺷﻴﻤﻴﺎﻳﻲ و ﺗﻜﻨﻴﻚ PCRاﻧﺠﺎم ﺷﺪ. ﻣﻘﺎوﻣﺖ آﻧﺘﻲﺑﻴﻮﺗﻴﻜﻲ ﺳﻮﻳﻪ اﺳـﺘﺎﻧﺪارد ﻻﻛﺘﻮﺑﺎﺳـﻴﻠﻮس ﭘﻼﻧﺘـﺎروم و ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﻛﺎزﻳﻲ ﺑﺮرﺳﻲ ﺷﺪ. ﺳﭙﺲ، ﻓﻌﺎﻟﻴﺖ ﻣﻬﺎرﻛﻨﻨﺪﮔﻲ ﻣﺤﻠﻮل روﻳﻲ ﻛﺸﺖ ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﭘﻼﻧﺘﺎروم و ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﻛﺎزﻳﻲ ﻋﻠﻴﻪ ﺑﻮرﺧﻮﻟﺪرﻳﺎ ﺳﭙﺎﺷﻴﺎ، در دو ﺣﺎﻟﺖ ﻓﻌﺎل و ﻏﻴﺮﻓﻌﺎل، ﺑﻪ روش ﭼﺎﻫﻚ درآﮔﺎر، ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮارﮔﺮﻓﺖ.
ﻳﺎﻓﺘﻪ ﻫﺎ: در اﻳﻦ ﺗﺤﻘﻴﻖ، ﺗﻌﺪاد 5 ﺑﺎﻛﺘﺮي ﺑﻮرﺧﻮﻟﺪرﻳﺎ ﺳﭙﺎﺷﻴﺎ از ﺧﻮن و 8 ﺑﺎﻛﺘﺮي از ﺧﻠﻂ ﺟﺪاﺳﺎزي ﺷـﺪ ﻧﺘـﺎﻳﺞ ﺣﺴﺎﺳـﻴﺖ ﺑﺎﻛﺘﺮي ﺑﻮرﺧﻮﻟﺪرﻳﺎ ﺳﭙﺎﺷﻴﺎ ﻧﺴﺒﺖ ﺑﻪ ﺑﺎﻛﺘﺮي ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﻛﺎزﻳﻲ )66/67 ﺑﻴﺸﺘﺮ در وﺿﻌﻴﺖ ﺣﺪ واﺳـﻂ ﻗـﺮار داﺷـﺖ و ﺑﻌﺪ ﺑﻪ ﺗﺮﺗﻴﺐ در وﺿﻌﻴﺖ ﻣﻘﺎوم و ﺣﺴﺎس و ﻧﺴﺒﺖ ﺑﻪ ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﭘﻼﻧﺘـﺎروم ﻧﻴـﺰ ﺑﻴﺸـﺘﺮ در وﺿـﻌﻴﺖ ﺣـﺪ واﺳﻂ و ﺳﭙﺲ ﺑـﻪ ﺗﺮﺗﻴـﺐ ﺣﺴـﺎس و ﻣﻘـﺎوم ﺑﻮدﻧـﺪ ﻻﻛﺘﻮﺑﺎﺳـﻴﻠﻮس ﻛـﺎزﻳﻲ ﺑـﻪ ﻫﻤـﻪ آﻧﺘـﻲ ﺑﻴﻮﺗﻴﻜﻬـﺎ ﻣﻘـﺎوم ﺑـﻮد ﺑـﻪ ﺟـﺰ ﻛﻮﺗﺮﻳﻤﻮﻛﺴﺎزول ﻛﻪ در وﺿﻴﻌﺖ ﺣﺪ واﺳﻂ ﻗﺮار داﺷﺖ و ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﭘﻼﻧﺘﺎروم ﺑﻪ آﻧﺘﻲﺑﻴﻮﺗﻴﻚ ﺟﻨﺘﺎﻣﻴﺴـﻴﻦ ﺣﺴـﺎس ، ﺑـﻪ آﻧﺘﻲﺑﻴﻮﺗﻴﻚ ﻛﻮﺗﺮﻳﻤﻮﻛﺴﺎزول در وﺿﻌﻴﺖ ﺣﺪ واﺳﻂ و ﺑﻪ آﻧﺘﻲﺑﻴﻮﺗﻴﻜﻬﺎي دﻳﮕﺮ ﻣﻘﺎوم ﺑﻮد.
ﻧﺘﻴﺠﻪ ﮔﻴﺮي: اﻳﻦ ﺗﺤﻘﻴﻖ ﻧﺸﺎن داد ﻛﻪ ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﭘﻼﻧﺘﺎروم و ﻻﻛﺘﻮﺑﺎﺳﻴﻠﻮس ﻛﺎزﻳﻲ ﺑﺮ ﻋﻠﻴﻪ ﺑﻮرﺧﻮﻟﺪرﻳﺎ ﺳﭙﺎﺷﻴﺎ اﺛﺮ ﻣﻬـﺎري دارﻧﺪ
چكيده لاتين :
Background: Nosocomial infections are the causes of the most common of morbidity and
mortality, prolonged hospital stay, and increased hospital costs worldwide. Today
probiotic bacteria, as antibacterial agents, attracted the attention of clinicians. The current
study aimed at determining the antibacterial activity of Lactobacillus plantarum and L.
casei species against Burkholderia cepacia isolated from Imam Ali (AS) Hospital, Amol,
Iran.
Methods: In the current study, B. cepacia species isolated from Imam Ali (AS) Hospital
were identified using bacterial culture and biochemical tests as well as polymerase chain
reaction (PCR) technique, and the antibacterial activity of L. plantarum and L. casei
standard species against the isolated species of B. cepacia were assessed. Then, the
inhibitory activity of L. plantarum and L. casei broth cultures supernatants in two active
and inactive forms against B. cepacia was also assessed using the agar well diffusion.
Results: In the current study, five isolates of B. cepacia were isolated from blood and
eight from sputum samples. Sensitivity of B. cepacia against L. casei was mostly
moderate (66.67%) followed by resistant and sensitive; but it was moderate against L.
plantarum, followed by sensitive and resistant. Lactobacillus casei was resistant against
all the examined antibiotics, except cotrimoxazole in moderate level; L. plantarum was
sensitive to gentamycin, moderate to cotrimoxazole, and resistant against other antibiotics.
Conclusion: Results of the current study showed that L. plantarum and L. casei have
inhibitory effects against B. cepacia.
عنوان نشريه :
نشريه دانشگاه علوم پزشكي البرز
