اثر عصاره جلبك دوناليلا سالينا برروي ميزان بيان ژن Bax در رده سلولي سرطان گليوبلاستوما

The effect of extract of Dunaliella salina L. on the expression of Bax in glioblastoma cancer cell Line

عصاره هاي گياهان مي توانند ميزان بيان ژن ها را در سلول هاي سرطاني تغيير دهند. تا به حال، مطالعه اي در رابطه با اثر عصاره جلبك ها انجام نشده است. اين مطالعه با هدف بررسي ميزان تغيير بيان ژن Bax و تعيين مقدار زنده ماني سلول هاي سرطان مغز تيمار شده با عصاره جلبك دوناليلا سالينا انجام شد. روش ها رده سلولي U87 از مركز ذخاير زيستي و ژنتيكي ايران تهيه و كشت داده شد. سلول ها به دو گروه تيمار و كنترل تقسيم شدند. عصاره جلبك دوناليلا روي گروه تيمار با غلظت هاي 0/5 ،1 ،2 ،5 ،10 ،15 ،20 ،50 و 100 ميلي گرم بر ميلي ليتر تاثير داده شد و سميت آن با استفاده از MTT assay سنجيده شد. بيانBax با روشReal time PCR بررسي شد. RNA از سلول هاي گروه تيمار و كنترل استخراج و cDNA ساخته شد. ميزان بيان ژن ها با استفاده ازReal-time PCR و Ct∆∆ مورد بررسي قرار گرفت. نتايج نتيجهMTT نشان داد كه IC50 در 48 ساعت تيمار، mg/ml 10/01 مي باشد. با انجامReal Time PCR مشخص شد كه در اين سلول ها، ميزان بيان ژن آپوپتوزي Bax به ميزان 4 برابر (0/001

Plant extracts can change the expression of genes in cancer cells. Till now, no study has been performed on the effect of algae extract. The aim of this research was to study the changes in Bax apoptotic gene expression in glioblastoma cell line and to determine the rate of cell death in cancer cells treated with Dunalilla salina extract.U87 cell line contains glioblastoma cancer cells as the most acute type of human brain tumor. Several genes, including Bax, play important roles in initiating apoptosis and the expression of Bax decreases during cancer. Today, because of the side effects of chemical drugs, the tendency to use algae and medicinal plants in the treatment of diseases has increased. Methods The U87 cell line was prepared from Iranian Biological Resources Center and cultivated. Cells were divided into two treatment and control groups. Dunaliella extract was affected on the treatment group with concentrations of 0.5, 1, 2, 5, 10, 15, 20, 50 and 100 mg/ml and its toxicity was measured using MTT assay. Bax expression was examined by Real time PCR. RNA was extracted from the cells of the treatment and control group and the cDNA was made. The expression of genes was evaluated using Real time PCR and ΔΔCt. Results The result of MTT indicated that IC50 was 10.01 mg/ml for 48 hours of treatment. By using Real Time PCR, it was found that in these cells, the expression level of Bax apoptotic gene increased 4-fold (p-value <0.001). Conclusion Treatment with algae extract was able to increase gene expression and kill cancer cells. It is suggested that the effect of extracts of other algae on changes in the expression of other genes involved in apoptosis be investigated.