اثر چهار هفته تمرين مقاومتي برسطوح پروتئين ABCA1 كبدي و سطوح HDL-C پلاسمايي موش هاي نر ويستار

The Effect of 4 week resistance training on male rat hepatic ABCA1 protein plasma HDL-C levels

هدف: ABCA1 عضوي از خانواده ناقل‌هاي‌ غشايي ABC در پستانداران است كه نقشي اساسي در نوآراييHDL-C و پيشگيري از بيماري آترواسكلروزيس (تصلب شرائين) دارد. هدف از پژوهش بررسي تغييرات ABCA1 كبدي و سطوح پلاسمايي HDL-C موش‌هاي نر ويستار به دنبال چهار هفته تمرين مقاومتي است. روش شناسي: 16موش صحرايي نر ويستار 14-12 هفته‌اي با ميانگين وزن290 گرم در دو گروه كنترل (8n=) و تمرين (8n=) در اين پژوهش مورد استفاده قرار گرفت. تمرين مقاومتي شامل بالا رفتن از يك نردبان يك متري به همراه وزنه بسته شده به دم حيوانات بود. موش ها 48 ساعت بعد از آخرين جلسه تمريني قرباني شد. بافت كبد نمونه برداري شده فوراً پس از شستشو با سرم فيزيولوژيك توسط نيتروژن مايع فريز، و به يخچال با دماي C 70- انتقال يافت. پلاسما نيز جهت اندازه‌گيري HDL وapoA-I (آپوليپوپروتئين اصلي HDL) از نمونه خوني كه به طور مستقيم از قلب گرفته شده بود، جداسازي شد. براي بررسي ميزان پروتئين ABCA1و apoA-I از كيت الايزا و براي اندازه ­گيري سطح HDL از روش ايمني آنزيمي استفاده شد. يافته‌ها: نتيجه نشان داد كه ميزان ABCA1در كبد موش‌هاي تمرين كرده حدود 25 درصد بالاتر از گروه كنترل بود، اما اين اختلاف از نظر آماري معني‌دار نبود (0/05

Aim:ABCA1, a member of ABC (ATP-binding cassette transporters) family in mammals plays a fundamental role in HDL-C remodeling and thus prevent atherosclerosis. The purpose of this study was to investigate the changes of hepatic ABCA1 and plasma of HDL-C levels of male Wistar rats following four weeks of resistance training. Methods: 16 male Wistar rats with the age of 12-14 weeks and a mean body weight of 290± 10g were used. Rats were divided in Two groups of control (n=8) and exercise (n=8). Resistance training consisted of climbing a one meter ladder (26 stairs and 80 degree angle with the ground) with weights tied to the tail of the animal. Finally, rats performed three consecutive sets with 100% of their body weights (6 reps per set). Rats were sacrificed 48 h after the last training session. Sampled Liver tissue, after washing with saline, were immediately frozen by liquid nitrogen. Finally, samples were transferred to -70°C. Blood samples were directly taken from the heart and the plasma were isolated for measurement of the HDL and apoA-I (the major apolipoprotein of HDL). ELISA kit was used to evaluate the amount of ABCA1 and apoA-I and Plasma HDL level was determined by enzymatic immune method. For data analysis, independent T test was used. Results: The results showed that ABCA1 in the liver of rats was higher for about 25 percent higher than the control group, but was not statistically significa (P>0/05). Plasma HDL levels were not significantly different between two groups (P>0/05). Moreover, despite some increases in apoA-I levels they were not statistically significant (P>0/05). Conclusions: Thus, despite the fact that four weeks of resistance training didn’t make statistically significant changes in ABCA1 and HDL and apoA-I, but according to the trend of the data this resistance training protocol seemed to be partly effective Possibly, better results can be achieved by increasing the intensity and leng of the execises, or by designing a beter combination of exercises.