مطالعه تغييرات ايجادشده در پروتئين‌هاي فاز حاد سرم، هموگرام و الگوي الكتروفورتيكي پروتئين‌هاي سرمي اسب متعاقب تغيير جيره غذايي از علوفه به علوفه مخلوط با كنسانتره

Alterations in acute phase proteins, hemogram and electrophoretic pattern of equine serum proteins following change of diet from forage to forage mixed with concentrate

ﻋﻠﯽرﻏﻢ ﻣﻄﺎﻟﻌﺎت ﮔﺴﺘﺮدهاي ﮐﻪ در زﻣﯿﻨﻪ ارﺗﺒﺎط ﻧﻮع ﺟﯿﺮه و ﺗﺄﺛﯿﺮ آن ﺑﺮ ﻓﺎﮐﺘﻮرﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ اﺳﺐ ﺻﻮرت ﮔﺮﻓﺘﻪ، اﻣﺎ اﻃﻼﻋﺎت ﮐﻤﯽ در ﻣﻮرد ﭘﺮوﺗﺌﯿﻦﻫﺎي ﺳﺮم و ﭘﻼﺳﻤﺎي اﺳﺐ ﺑﻪ دﻧﺒﺎل ﺗﻐﯿﯿﺮ ﺟﯿﺮه ﻏﺬاﯾﯽ از ﻧﻮع ﻋﻠﻮﻓـﻪ ﺑـﻪ ﻋﻠﻮﻓـﻪ ﻣﺨﻠـﻮط ﺑـﺎ ﮐﻨﺴـﺎﻧﺘﺮه وﺟـﻮد دارد. ﭘﺮوﺗﺌﯿﻦﻫﺎي ﻣﺮﺣﻠﻪ ﺣﺎد ﮔﺮوﻫﯽ از ﭘﺮوﺗﺌﯿﻦﻫﺎي ﺳﺮم ﻫﺴﺘﻨﺪ ﮐﻪ ﻏﻠﻈﺖ آنﻫﺎ در ﭘﺎﺳﺦ ﺑﻪ ﻋﻔﻮﻧﺖﻫﺎ، اﻟﺘﻬﺎب، ﺗﺮوﻣﺎ و ﻧﺌـﻮﭘﻼزي اﻓـﺰاﯾﺶ ﻣﯽﯾﺎﺑﺪ. از اﯾﻦ رو، در ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ﺑﻪ ارزﯾﺎﺑﯽ ﺳﻄﺢ ﺳﺮﻣﯽ ﻧﺸﺎنﮔﺮﻫﺎي زﯾﺴﺘﯽ ﺳﺮم، ﻓﯿﺒﺮﯾﻨﻮژن و ﻫﻤﭽﻨﯿﻦ ﻏﻠﻈـﺖ ﭘـﺮوﺗﺌﯿﻦ ﮐـﻞ ﺑـﻪ ﻫﻤﺮاه ارزﯾﺎﺑﯽ رﻓﺘﺎر ﺗﻐﺬﯾﻪاي اﺳﺐﻫﺎ در ﻫﻨﮕﺎم ﺗﻐﯿﯿﺮ ﺟﯿﺮه ﻏﺬاﯾﯽ ﭘﺮداﺧﺘﯿﻢ. ﺑﺪﯾﻦ ﻣﻨﻈﻮر، دو ﻧﻮع ﺟﯿﺮه ﻏﺬاﯾﯽ، ﺷﺎﻣﻞ ﻋﻠﻮﻓﻪ )ﺗﯿﻤﺎر اول( و ﻋﻠﻮﻓﻪ ﻣﺨﻠﻮط ﺑﺎ ﮐﻨﺴﺎﻧﺘﺮه )ﺗﯿﻤﺎر دوم( ﺑﺮاي ﻫﺮ اﺳﺐ ﺑﻪ ﻣﺪت ﭘﺎﻧﺰده روز ﻣﺘﻮاﻟﯽ اﺳﺘﻔﺎده ﺷﺪ. ﺷﻤﺎرش ﮐﺎﻣـﻞ ﺳـﻠﻮل ﻫـﺎي ﺧـﻮن و ﻫﻤﭽﻨﯿﻦ اﻧﺪازهﮔﯿﺮي ﺳﻄﺢ ﺳﺮﻣﯽ ﻓﯿﺒﺮﯾﻨﻮژن، ﻫﺎﭘﺘﻮﮔﻠﻮﺑﯿﻦ و آﻣﯿﻠﻮﺋﯿﺪ ﻧﻮع A ﻗﺒﻞ و ﺑﻌﺪ از ﺗﻐﯿﯿﺮ رژﯾﻢ ﻏﺬاﯾﯽ اﻧﺪازهﮔﯿـﺮي ﺷـﺪ. ﺳـﻄﺢ ﻓﯿﺒﺮﯾﻨﻮژن، ﻫﺎﭘﺘﻮﮔﻠﻮﺑﯿﻦ و آﻣﯿﻠﻮﺋﯿﺪ A ﺳﺮم ﺑﻪ ﻃﻮر ﻣﻌﻨﯽداري در اﺳﺒﺎن ﮔﺮوه ﺗﯿﻤﺎر دوم در ﻣﻘﺎﯾﺴﻪ ﺑﺎ اﺳﺐﻫﺎي ﮔﺮوه ﺗﯿﻤﺎر اول اﻓـﺰاﯾﺶ داﺷﺖ )0/05

Despite extensive studies on the relationship between diet type and its effect on equine biochemical factors, little is known about horse serum and plasma proteins following changes in diet from forage to forage mixed with concentrate. Acute phase proteins are a group of serum proteins whose concentrations increase in response to infections, inflammation, trauma, and neoplasia. Therefore, in the present study, we evaluated the serum levels of serum biomarkers, fibrinogen and total protein concentration along with the nutritional behavior of horses during diet change. For this purpose, two types of diets, including forage (first treatment) and mixture of forage with concentrate (second treatment) were used for each horse for fifteen consecutive days. Complete blood cell counts as well as serum levels of fibrinogen, haptoglobin and amyloid type A were measured before and after dietary changes. Serum fibrinogen, haptoglobin and amyloid A levels were significantly increased in horses of the second treatment group compared to horses in the first treatment group (p<0.05). There was also a statistically significant difference in the complete blood cell count due to the change of diet from forage to forage mixed with concentrate (p<0.05). It seems that changes in the natural flora of the horse's digestive tract following a change in diet can protect the animal's body against acute inflammatory and traumatic injuries.