تأثير مورفومتري، ويسكوزيته و آبكي‌شدن مني بر كيفيت اسپرم منجمد قوچ قزل

The effect of morphometry, viscosity and liquefaction on frozen sperm quality of Ghezel ram

ﺗﻮاﻧﺎﯾﯽ اﺳﭙﺮم در ﺣﺮﮐﺖ و رﺳﯿﺪن ﺑﻪ اووﺳﯿﺖ ﺟﻬﺖ اﻧﺠﺎم ﻟﻘﺎح و ﺑﺎروري ﺑﻪ ﻋﻮاﻣـﻞ ﻣﺨﺘﻠﻔـﯽ از ﺟﻤﻠـﻪ ﻣﻮرﻓـﻮﻣﺘﺮي، آﺑﮑـﯽﺷـﺪن و وﯾﺴﮑﻮزﯾﺘﻪ ﻣﻨﯽ ﺑﺴﺘﮕﯽ دارد. ﻣﻄﺎﻟﻌﻪء ﺣﺎﺿﺮ ﺑﻪ ﻣﻨﻈﻮر ارزﯾﺎﺑﯽ ﺗﺄﺛﯿﺮ ﻣﻮرﻓﻮﻣﺘﺮي، وﯾﺴﮑﻮزﯾﺘﻪ و آﺑﮑﯽﺷﺪن ﻣﺎﯾﻊ ﻣﻨﯽ ﺑـﺮ ﮐﯿﻔﯿـﺖ اﺳـﭙﺮم ﻣﻨﺠﻤﺪ ﻗﻮچ ﻗﺰل اﻧﺠﺎم ﮔﺮﻓﺖ. ﺟﻤﻊآوري ﻣﻨﯽ ﯾﮑﺒﺎر در ﻫﻔﺘﻪ ﺑﻪ ﻣﺪت 3 ﻫﻔﺘﻪ از 5 رأس ﻗﻮچ ﻗﺰل ﺻﻮرت ﮔﺮﻓﺖ. ارزﯾـﺎﺑﯽﻫـﺎي اوﻟﯿـﻪ ﺷﺎﻣﻞ ﺗﺤﺮك ﮐﻞ و ﭘﯿﺸﺮوﻧﺪه، درﺻﺪ زﻧﺪهﻣﺎﻧﯽ، درﺻﺪ اﺳﭙﺮمﻫﺎي ﻏﯿﺮﻃﺒﯿﻌـﯽ، ﻏﻠﻈـﺖ، وﯾﺴـﮑﻮزﯾﺘﻪ، ﻣﻮرﻓـﻮﻣﺘﺮي و آﺑﮑﯽﺷـﺪن ﺑـﻮد. ﻧﻤﻮﻧﻪﻫﺎي ﺑﺎ ﻏﻠﻈﺖ ﺑﺎﻻي 2/5 ﻣﯿﻠﯿﺎرد اﺳﭙﺮم و ﺣﺮﮐﺖ ﭘﯿﺸﺮوﻧﺪهء ﺑﺎﻻي 70 درﺻﺪ ﺑﺮاي رﻗﯿﻖﺳﺎزي ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﺮﻓﺘﻨـﺪ. ﺑﻌـﺪ از رﻗﯿﻖﺳﺎزي، ﻧﻤﻮﻧﻪﻫﺎ در داﺧﻞ ﭘﺎﯾﺖﻫﺎي 0/25 ﻣﯿﻠﯽﻟﯿﺘﺮي ﮐﺸﯿﺪه ﺷﺪه و در ﯾﺨﭽﺎل ﺑﻪ ﻣﺪت 1/5-2 ﺳﺎﻋﺖ ﺟﻬﺖ ﺳﺮدﺳﺎزي ﺗﺎ دﻣـﺎي 5 درﺟﻪء ﺳﻠﺴﯿﻮس ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﺳﭙﺲ ﺑﻪ ﻣﺪت 8-10 دﻗﯿﻘﻪ در 4-5 ﺳﺎﻧﺘﯽﻣﺘﺮي ﺑﺎﻻي ﺳﻄﺢ ﻧﯿﺘﺮوژن ﻣﺎﯾﻊ در ﺑﺨﺎر ازت ﻗﺮار ﮔﺮﻓﺘﻨﺪ و در ﻧﻬﺎﯾﺖ داﺧﻞ ﻧﯿﺘﺮوژن ﻣﺎﯾﻊ ﻏﻮﻃﻪور ﺷﺪﻧﺪ. ﯾﺦﮔﺸﺎﯾﯽ ﻧﻤﻮﻧﻪﻫﺎ در روزﻫﺎي ﺻﻔﺮ، 40 ،20 و 60 دوره ﻧﮕﻪداري، اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺻﻔﺎت ﻣﺮﺑﻮط ﺑﻪ ﺗﺤﺮك و زﻧﺪهﻣﺎﻧﯽ اﺳﭙﺮمﻫﺎ ﺑﺎ ﮔﺬﺷﺖ زﻣﺎن ﮐﺎﻫﺶ ﻣﻌﻨﯽداري داﺷﺘﻨﺪ )0/05

The ability of sperm to move and reach the oocyte for fertilization and fertility depends on different factors such as morphometry, liquefaction and semen viscosity. The present study was performed to evaluate the effect of morphometry, viscosity and liquefaction of semen on Ghezel ram frozen sperm quality. Semen was collected once a week for 3 weeks from 5 Ghezel rams. Initial assessments included total and progressive motility, viability, abnormal sperm percentage, concentration, viscosity, morphometry, and liquefaction. Samples with a concentration of 2.5 billion sperm and a progressive motility of over 70% were used for dilution. After dilution, straws (0.25 ml) were filled and after cooling and reaching the temperature of 5°C in the refrigerator, were placed 4-5 cm above liquid nitrogen for 8-10 min and then, ultimately were immersed in liquid nitrogen. The traits of motility and sperm health were assessed on days 0, 20, 40 and 60 of cryopreservation. The results showed that motility traits and viability were significantly reduced over time of cryopreservation (p<0.05). Furthermore, the results of this study showed a negative and significant correlation between drop length and plasma membrane health (p<0.05). Also, it was shown that there was a positive and significant correlation between semen string length and total motility (p<0.01). There was a significant negative correlation between liquefaction and total motility, progressive motility, viability and acrosome integrity (p<0.01). There was a significant negative correlation between tail length and total sperm length with plasma membrane health (p<0.01). The findings of the present study indicated that the shorter the liquefaction time, sperm tail length and total length of sperm, the qualitative traits of sperm will be better preserved in the freezing process.