اثرات حفاظتي و آنتي‌اكسيداني سيليمارين در گوسفندان مبتلا به فاسيولوزيس تحت درمان با كلرسولون

Protective and antioxidant effects of silymarin in sheep with fasciolosis treated by clorsulon

ﻓﺎﺳﯿﻮﻟﻮزﯾﺲ ﯾﮏ ﺑﯿﻤﺎري اﻧﮕﻠﯽ اﺳﺖ ﮐﻪ ﺳﺒﺐ آﺳﯿﺐﻫﺎي اﻗﺘﺼﺎدي ﻣﯽﺷﻮد. در ﻣﻄﺎﻟﻌﻪي ﺣﺎﺿﺮ ﺑﻪ ارزﯾﺎﺑﯽ اﺛـﺮات ﺣﻔـﺎﻇﺘﯽ داروي ﺳﯿﻠﯿﻤﺎرﯾﻦ ﺑﻪ ﺗﻨﻬﺎﯾﯽ و ﻫﻤﺮاه ﺑﺎ داروي ﮐﻠﺮﺳﻮﻟﻮن در ﮔﻮﺳﻔﻨﺪان آﻟﻮده ﺑﻪ اﻧﮕﻞ ﮐﺒﺪي ﻓﺎﺳﯿﻮﻻ ﻫﭙﺎﺗﯿﮑﺎ ﭘﺮداﺧﺘﻪ ﺷﺪ. ﺑﺪﯾﻦ ﻣﻨﻈـﻮر از 40 رأس ﮔﻮﺳﻔﻨﺪ ﻧﮋاد ﺳﻨﮕﺴﺮي در ﭘﻨﺞ ﮔﺮوه 8 رأﺳﯽ ﺷﺎﻣﻞ: 1- ﮔﻮﺳﻔﻨﺪان ﺳﺎﻟﻢ ﺑﺪون درﻣﺎن، 2- ﮔﻮﺳﻔﻨﺪان ﻣﺒﺘﻼ ﺑﻪ ﻓﺎﺳﯿﻮﻟﻮزﯾﺲ ﺑـﺪون درﻣﺎن، 3- ﮔﻮﺳﻔﻨﺪان ﻣﺒﺘﻼ ﺑﻪ ﻓﺎﺳﯿﻮﻟﻮزﯾﺲ ﺗﺤﺖ درﻣﺎن ﺑﺎ ﺳﯿﻠﯿﻤﺎرﯾﻦ، 4- ﮔﻮﺳﻔﻨﺪان ﻣﺒﺘﻼ ﺑﻪ ﻓﺎﺳﯿﻮﻟﻮزﯾﺲ ﺗﺤﺖ درﻣﺎن ﺑﺎ ﮐﻠﺮﺳﻮﻟﻮن و ﮔﻮﺳﻔﻨﺪان ﻣﺒﺘﻼ ﺑﻪ ﻓﺎﺳﯿﻮﻟﻮزﯾﺲ ﺗﺤﺖ درﻣﺎن ﺑﺎ داروﻫﺎي ﺳﯿﻠﯿﻤﺎرﯾﻦ و ﮐﻠﺮﺳـﻮﻟﻮن اﺳـﺘﻔﺎده ﺷـﺪ. ﭘﺎراﻣﺘﺮﻫـﺎي ﺑﯿﻮﺷـﯿﻤﯿﺎﯾﯽ ﺷـﺎﻣﻞ -5 ﺗﺮيﮔﻠﯿﺴﺮﯾﺪ، ﺑﯿﻠﯽروﺑﯿﻦ، ﭘﺮوﺗﺌﯿﻦ ﺗﺎم، ﮐﻠﺴﺘﺮول و آﻟﺒﻮﻣﯿﻦ ﺳﺮم و ﻫﻤﭽﻨﯿﻦ ﺳﻄﺢ ﺳﺮﻣﯽ ﻣﺎﻟﻮنديآﻟﺪﺋﯿﺪ، ﻇﺮﻓﯿﺖ ﺗـﺎم آﻧﺘﯽاﮐﺴـﯿﺪاﻧﯽ و ﻓﺎﮐﺘﻮرﻫﺎي ﻫﻤﺎﺗﻮﻟﻮژﯾﮑﯽ اﻧﺪازهﮔﯿﺮي و ﺑﯿﻦ ﮔﺮوهﻫﺎ ﻣﻘﺎﯾﺴﻪ ﺷﺪ. ﺟﻬﺖ آﺳﯿﺐﺷﻨﺎﺳﯽ ﺑﺎﻓﺘﯽ، ﻣﻘﺎﻃﻌﯽ از ﺑﺎﻓﺖ ﮐﺒﺪ ﺗﻬﯿـﻪ ﮔﺮدﯾـﺪ. ﺳـﻄﺢ ﺳﺮﻣﯽ ﮐﻠﺴﺘﺮول، ﺗﺮيﮔﻠﯿﺴﺮﯾﺪ، ﺑﯿﻠﯽروﺑﯿﻦ، ﭘﺮوﺗﺌﯿﻦ ﺗﺎم و آﻟﺒﻮﻣﯿﻦ ﮔﻮﺳﻔﻨﺪان ﻣﺒﺘﻼ ﺑﻪ ﻓﺎﺳـﯿﻮﻟﻮزﯾـﺲ ﺗﻮﺳـﻂ درﻣـﺎن ﺑـﺎ ﺳـﯿﻠﯿﻤﺎرﯾﻦ و ﮐﻠﺮﺳﻮﻟﻮن، ﺑﻪﻃﻮر ﻣﻌﻨﯽداري ﺑﻬﺒﻮد ﯾﺎﻓﺖ )0/05

Fasciolosis is a parasitic disease that causes economic losses. In the present study, the protective effects of silymarin alone and in combination with clorsulon were investigated in sheep naturally infected with Fasciola hepatica. For this purpose, 40 Sangsari breed sheep were used, which were divided into five groups, including 1- healthy sheep without treatment 2- fasciolosis affected sheep without treatment 3- fasciolosis affected sheep treated with silymarin 4- fasciolosis affected sheep treated with Clorsulon, and 5- fasciolosis affected sheep treated with silymarin and clorsulon. Biochemical parameters including triglyceride, total bilirubin, total protein, cholesterol, and albumin, were measured. The serum level of malondialdehyde, total antioxidant capacity, and hematological parameters were determined and compared among groups. Additionally, histopathological changes were investigated using light microscopy. Biochemical analysis showed significant improvement in bilirubin levels, total protein, cholesterol, and albumin in sheep treated with silymarin and clorsulon (p<0.05). Total antioxidant capacity increased in group treated with silymarin (p<0.05) compared to group 2, and the amount of oxidative stress decreased (p<0.01). Silymarin also improved hematologic factors and prevented a decrease in red blood cells (p<0.05). Liver inflammation, fibrosis, necrosis, and hyperplasia of the bile duct decreased significantly with the silymarin and clorsulon group compared to the other groups (p<0.0001). These findings showed that using silymarin in combination with clorsulon improved their therapeutic effects in treatment of ovine fasciolosis through enhancement of pharmacokinetic and pharmacodynamic relationship.