اثر باكوپا مونرو براختلال حافظه فضايي و پراكسيداسيون ليپيدي ناشي از تزريق موضعي اتيديوم برومايد در هيپوكامپ موش صحرايي

ﻤﺎري ﻣﺎﻟﺘﻴﭙﻞ اﺳﻜﻠﺮوزﻳﺲ و ﻧﻴﺰ ﺗﻮﻛﺴﻴﻦﻫﺎﻳﻲ ﻣﺎﻧﻨﺪ اﺗﻴﺪﻳﻮم ﺑﺮوﻣﺎﻳﺪ ﻣﻮﺟﺐ ﺗﺨﺮﻳﺐ ﻣﻴﻠﻴﻦ ﻧﻮرون ﻫﺎ ﻣﻲﺷﻮد. وﻳﮋﮔﻲﻫﺎي آﻧﺘﻲ- اﻛﺴﻴﺪاﻧﻲ ﺑﺎﻛﻮﭘﺎ ﻣﻮﻧﺮو در ﻣﻄﺎﻟﻌﺎت ﻗﺒﻠﻲ ﮔﺰارش ﺷﺪه اﺳﺖ. ﻫﺪف از اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﻲ ﺗﺪاﺧﻞ اﺛﺮ ﺑﺎﻛﻮﭘﺎ ﻣﻮﻧﺮو و اﺗﻴﺪﻳﻮم ﺑﺮوﻣﺎﻳﺪ ﺑﺮ ﺣﺎﻓﻈﻪ ﻓﻀﺎﻳﻲ و ﭘﺮاﻛﺴﻴﺪاﺳﻴﻮن ﻟﻴﭙﻴﺪي در ﺗﺸﻜﻴﻼت ﻫﻴﭙﻮﻛﺎﻣﭗ اﺳﺖ. اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮ روي 8 ﮔﺮوه 7 ﺗﺎﻳﻲ ﻣﻮشﻫﺎي ﺻﺤﺮاﻳﻲ ﻧﺮ ﺻﻮرت ﮔﺮﻓﺘﻪ اﺳﺖ. ﺳﻪ روز ﭘﺲ از ﺗﺰرﻳﻖ اﺗﻴﺪﻳﻮم ﺑﺮوﻣﺎﻳﺪ ﻳﺎ ﺑﺎﻛﻮﭘﺎ ﻣﻮﻧﺮو و ﻧﻴﺰ ﺗﺮﻛﻴﺐ اﻳﻦ داروﻫﺎ در ﻧﺎﺣﻴﻪ CA1 ﻫﻴﭙﻮﻛﺎﻣﭗ، آزﻣﻮن ﻣﺎز آﺑﻲ ﻣﻮرﻳﺲ ﺑﻪ ﻣﻨﻈﻮر ﺳﻨﺠﺶ ﭘﺎراﻣﺘﺮﻫﺎي ﺣﺎﻓﻈﻪ ﻓﻀﺎﻳﻲ ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮارﮔﺮﻓﺖ. ﺳﭙﺲ ﭘﺎراﻣﺘﺮ ﭘﺮاﻛﺴﻴﺪاﺳﻴﻮن ﻟﻴﭙﻴﺪي در اﻳﻦ ﻧﺎﺣﻴﻪ ﺳﻨﺠﺶ ﮔﺮدﻳﺪ. ﺗﺰرﻳﻖ 3 ﻣﻴﻜﺮوﻟﻴﺘﺮ اﺗﻴﺪﻳﻮم ﺑﺮوﻣﺎﻳﺪ 0/01 ﺑﻪ ﻧﺎﺣﻴﻪ CA1 ﻣﻮﺟﺐ ﻛﺎﻫﺶ ﭘﺎراﻣﺘﺮﻫﺎي ﻣﺮﺗﺒﻂ ﺑﺎ ﺣﺎﻓﻈﻪ ﻓﻀﺎﻳﻲ )0/01

Multiple sclerosis, as well as toxins such as ethidium bromide, can damage the myelin of neurons. The antioxidant properties of Bacopa monnieri have been reported in previous studies. The aim of this study was to investigate the interaction of Bacopa monnieri and ethidium bromide on spatial memory and lipid peroxidation in the hippocampal formation. This study was performed on eight groups of seven-member male rats. Three days after injection of ethidium bromide or Bacopa monnieri, and also combination of these drugs in the CA1 region of the hippocampus, Morris Water Maze test was employed to measure spatial memory parameters. Then the lipid peroxidation parameter was measured in this region. bromide into the CA1 region Injection 3 μL of ethidium bromide 1% reduced the parameters related to spatial memory (P <0.01) and increased lipid peroxidation (P <0.01). In addition, administration of the subthreshold dose of 5 μg/rat of Bacopa monnieri stopped the effect of three–days injection of ethidium bromide on parameters of spatial memory (P <0.01, P <0.001) and decreased lipid peroxidation (P <0.01). Bacopa monnieri can improve spatial memory by decreasing the amount of lipid peroxidation induced by local injection of ethidium bromide.