ﺗﻌﯿﯿﻦ اﻟﮕﻮي ﻣﻘﺎوﻣﺖ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮑﯽ ﺟﺪاﯾﻪ ﻫﺎي اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ O157:H7 از ﻫﻤﺒﺮﮔﺮ در اﺳﺘﺎن ﻣﺎزﻧﺪران

Determination of antibiotic resistance pattern of Escherichia coli O157:H7 isolates from hamburger in Mazandaran province

اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ O157:H7 ﯾﮑﯽ از ﻋﻮاﻣﻞ ﺑﺎﻟﻘﻮه در ﺑﺮوز ﺑﯿﻤﺎري ﻫﺎي ﻏﺬازاد اﺳﺖ. ﻧﻘﺶ ﮔﻮﺷﺖ ﮔﺎو ﺑﻪ ﻋﻨﻮان ﻣﻨﺒﻊ ﺳﻮﯾﻪ ﻫﺎي اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ O157:H7 ﺷﻨﺎﺧﺘﻪ ﺷﺪه اﺳﺖ. ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ﺑﻪ ﻣﻨﻈﻮر ارزﯾﺎﺑﯽ ﻣﯿﺰان ﺷﯿﻮع و ﻣﻘﺎوﻣﺖ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮑﯽ ﺟﺪاﯾﻪ ﻫﺎي اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ O157:H7 ﺟﺪا ﺷﺪه از ﻧﻤﻮﻧﻪ ﻫﺎي ﻫﻤﺒﺮﮔﺮ ﺟﻤﻊ آوري ﺷﺪه از اﺳﺘﺎن ﻣﺎزﻧﺪران اﻧﺠﺎم ﭘﺬﯾﺮﻓﺖ. دوﯾﺴﺖ ﻧﻤﻮﻧﻪ ﻫﻤﺒﺮﮔﺮ ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﯽ از ﻣﺮاﮐﺰ ﻓﺮوش اﺳﺘﺎن ﻣﺎزﻧﺪران ﺟﻤﻊ آوري ﺷﺪ. ﺗﺎﯾﯿﺪ اﯾﺰوﻟﻪ ﻫﺎي اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ O157:H7 رﺷﺪ ﯾﺎﻓﺘﻪ در ﻣﺤﯿﻂ ﮐﺸﺖ ﻣﯿﮑﺮوﺑﯽ ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن ﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ و واﮐﻨﺶ PCR اﻧﺠﺎم ﭘﺬﯾﺮﻓﺖ. اﻟﮕﻮي ﻣﻘﺎوﻣﺖ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮑﯽ ﺟﺪاﯾﻪ ﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از روش دﯾﺴﮏ اﻧﺘﺸﺎري آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ ارزﯾﺎﺑﯽ ﺷﺪ. ﺑﺮ ﻃﺒﻖ ﻧﺘﺎﯾﺞ، 19 ﻧﻤﻮﻧﻪ از ﮐﻞ 200 ﻧﻤﻮﻧﻪ )9/50 درﺻﺪ( ﻫﻤﺒﺮﮔﺮ ﺟﻤﻊ آوري ﺷﺪه از اﺳﺘﺎن ﻣﺎزﻧﺪران، آﻟﻮده ﺑﻪ اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ O157:H7 ﺑﻮدﻧﺪ. ﺳﻮش ﻫﺎي اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ O157:H7 ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان ﻣﻘﺎوﻣﺖ را ﺑﺮ ﻋﻠﯿﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ ﻫﺎي ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ )100 درﺻﺪ(، آﻣﭙﯽ ﺳﯿﻠﯿﻦ )100 درﺻﺪ(، ﺟﻨﺘﺎﻣﺎﯾﺴﯿﻦ )89/47 درﺻﺪ(، ﺳﻮﻟﻔﺎﻣﺘﻮﮐﺴﺎزول )73/68 درﺻﺪ( و ﺳﯿﭙﺮوﻓﻠﻮﮐﺴﺎزﯾﻦ )73/68 درﺻﺪ( داﺷﺘﻨﺪ. ﮐﻤﺘﺮﯾﻦ ﻣﯿﺰان ﻣﻘﺎوﻣﺖ ﺑﺮ ﻋﻠﯿﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ ﻫﺎي ﮐﻠﺮاﻣﻔﻨﯿﮑﻞ )15/78 درﺻﺪ( و ﺳﻔﻮﺗﺎﮐﺴﯿﻢ )42/10 درﺻﺪ( ﺑﺪﺳﺖ آﻣﺪ. ﻫﻤﺒﺮﮔﺮ ﺑﻪ ﻋﻨﻮان ﻣﻨﺒﻊ اﻧﺘﻘﺎل ﺳﻮﯾﻪ ﻫﺎي ﻣﻘﺎوم ﺑﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ اﺷﺮﯾﺸﯿﺎ ﮐﻠﯽ O157:H7 در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷد

Escherichia coli O157:H7 is one of the potential causes of foodborne diseases. The role of cattle meat is known as the source of Escherichia coli strains O157:H7. The present study was performed to evaluate the prevalence and antibiotic resistance of Escherichia coli O157:H7 strains isolated from hamburger samples collected from Mazandaran province. Two-hundred samples of hamburgers were randomly collected from the shopping centers of Mazandaran province. Confirmation of Escherichia coli O157:H7 isolates grown in microbial culture medium was performed using biochemical tests and PCR reaction. The pattern of antibiotic resistance of the isolates was evaluated using the antibiotic disk diffusion method. According to the results, 19 out of 200 samples (9.50%) of hamburger collected from Mazandaran province were contaminated with Escherichia coli O157:H7. Escherichia coli O157:H7 strains had the highest resistance to tetracycline (100%), ampicillin (100%), gentamicin (89.47%), sulfamethoxazole (73.68%) and ciprofloxacin (73.68%) antibiotics. The lowest resistance was obtained against chloramphenicol (15.78%) and cefotaxime (42.10%) antibiotics. Hamburger was considered as a source of transmission of antibiotic- resistant Escherichia coli O157:H7 strains.