تعيين تركيبات شيميايي اسانس و بررسي فعاليت آنتي اكسيداني عصاره گياه بادرنجبويه بهشهر

Analyzing of chemical composition of the essential oil and study of antioxidant activity of extract of Melissa officinalis from Behshahr -Iran

اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﻪﻣﻨﻈﻮر آﻧﺎﻟﯿﺰ ا ﺳﺎﻧﺲ و ﻋ ﺼﺎره ي اﺗﺎﻧﻮﻟﯽ و ﺑﺮر ﺳﯽ ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐ ﺴﯿﺪاﻧﯽ ﻋ ﺼﺎره ﻫﺎي اﺗﺎﻧﻮﻟﯽ و n-ﻫﮕﺰاﻧﯽ ﺑﺮگ ﮔﯿﺎه ﺑﺎدرﻧﺠﺒﻮﯾﻪ )Melissa officinalis ( ﻣﻨﻄﻘﻪ ﻫﺰارﺟﺮﯾﺐ ﺑﻬ ﺸﻬﺮ ﺑﻪ ﻋﻨﻮان ﺟﺎﯾﮕﺰﯾﻦ آﻧﺘﯽ اﮐ ﺴﯿﺪان ﻫﺎي ﺳﻨﺘﺰي اﻧﺠﺎم ﺷﺪ. ﺑﺮگ ﮔﯿﺎه از روﯾ ﺸﮕﺎه ﻫﺎي ﻃﺒﯿﻌﯽ آن در ارﺗﻔﺎﻋﺎت ا ﺳﺘﺎن ﻣﺎزﻧﺪران و در اواﯾﻞ ﺷﻬﺮﯾﻮر ﻣﺎه ﺟﻤﻊ آوري ﺷﺪ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺗﻌﯿﯿﻦ ﺗﺮﮐﯿﺒﺎت ﺷﯿﻤﯿﺎﯾﯽ اﺳﺎﻧ ﺲ و ﻋﺼﺎره اﺗﺎﻧﻮﻟﯽ ﺑﺮگ ﮔﯿﺎه ﺑﺎ دﺳﺘﮕﺎه GC/MS ﺑﺮرﺳﯽ ﺷﺪ. ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﻋﺼﺎره ﻫﺎي اﺗﺎﻧﻮﻟﯽ و n-ﻫﮕﺰاﻧﯽ ﺑﺎ اﺳﺘﻔﺎده از روش DPPH ﺑﺮرﺳﯽ و ﺑﺎ آﻧﺘﯽ اﮐﺴﯿﺪان ﺳﻨﺘﺰي آﺳﮑﻮرﺑﯿﮏ اﺳﯿﺪ ﻣﻘﺎﯾﺴﻪ ﺷﺪ ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ اﺟﺰاي اﺻﻠﯽ اﺳﺎﻧﺲ ﺑﻪ ﺗﺮﺗﯿﺐ ﻓﻨﻮﮐﺴﯽ اﺗﺎﻧﻮل ) 31/66% (، ﻣﺠﻤﻮع اﻧﺎﻧﺘﯿﻮﻣﺮ ﮐﺎرﯾﻮﻓﯿﻠﻦ اﮐﺴﺎﯾﺪ ) 24/71%(، ﻣﺠﻤﻮع اﻧﺎﻧﺘﯿﻮﻣﺮ ﺳﯿﺘﺮال ) 13/89% ( و ﻣﺠﻤﻮع اﻧﺎﻧﺘﯿﻮﻣﺮ ﮐﺎرﯾﻮﻓﯿﻠﻦ )9/88%( و اﺟﺰاي اﺻــﻠﯽ ﻋﺼــﺎره اﺗﺎﻧﻮﻟﯽ ﺑﻪ ﺗﺮﺗﯿﺐ ﮐﺎرﯾﻮﻓﯿﻠﻦ اﮐﺴــﺎﯾﺪ )2- ،(%28/95 هيدرازينو نيكوتينيك اسيد (11/37%)، فيتول (61/8%) و بتا-كاريوفيلن (7/44%) مي باشند. همچنين Ic50 عصاره هاي اتانولي و ان- هگزاني به ترتيب 39.05 , 98.93 ميكروگرم بر ميلي ليتر و در روش FRAP توان احيا كنندگي عصاره هاي اتانولي و ان- هگزاني به ترتيب 91.3 , 105.5 ميكرو گرم بر ميلي ليتر تعيين شدند. با مقايسه نتايج بدست آمده، عصاره ها داراي خاصيت آنتي اكسيداني و توانايي احيا كنندگي بوده و مي توانند در صنعت دارويي بكار روند.

This research has been conducted to analyzeessential oil and ethanol extract and investigate the antioxidant activity of the ethanol and n-hexane extracts of the Melissa officinalis leaves collected from Hezar Jarib area of Behshahr. Melissa officinalis leaf can be considered as a substitute of the synthetic antioxidants. The leaves of the plant were collected from its natural habitats in the heights of Mazandaran province in early September. In this study, chemical composition of the essential oil and ethanol extract of the Melissa officinalis leaf were analyzed by GC/MS. The antioxidant activity of the ethanol and n-hexane extracts is investigated using method of DPPH and compared to the synthetic antioxidant of ascorbic acid. The results showed that the main components of essential oil are: Phenoxyethanol (31.66%), total of Carryophyllene Oxide enantiomer (24.71%), totoal of Citral enantiomer (13.89%) and totoal of Caryophyllene enantiomer (9.88%) also the main components of ethanol extract are: Carryophyllene Oxide (28.95%), totoal of 2-Hydrazino–Nicotinic acid (11.37%), Phytol (8.61%) and β-Caryophyllene (7.44%) respectively. Also the Ic50 of the ethanol and n-hexane extracts are determined 39.05 and 98.93 μg/ml respectively, and in the FRAP method the ability of reducing of the ethanol and n-hexane extracts were 91.3 and 105.5 μg/ml respectively. By comparing the results, extracts have antioxidant effect and ability of reducing so can be used in medicine industries.