كليدواژه :
كالوس زايي , سوسپانسيون سلولي , Portulaca oleracea L , گياه دارويي خرفه
چكيده فارسي :
در اﯾﻦ ﺗﺤﻘﯿﻖ، ﺗﺄﺛﯿﺮ ﻧﻮع و ﻏﻠﻈﺖ دو ﺗﻨﻈﯿﻢ ﮐﻨﻨﺪه رﺷﺪ ﮔﯿﺎﻫﯽ BAP و D-2,4 ﺑﺮ ﮐﺎﻟﻮس زاﯾﯽ رﯾﺰﻧﻤﻮﻧﻪ ﻫﺎي ﻫﯿﭙﻮﮐﻮﺗﯿﻞ و ﺑﺮگ در ﻣﺤﯿﻂ ﮐﺸﺖ MS ﺣﺎوي 1 ،0/5 و 2 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ D-2,4 و BAP و ﺑﻬﯿﻨﻪ ﺳﺎزي ﮐﺸﺖ ﺳﻮﺳﭙﺎﻧﺴﯿﻮن ﺳﻠﻮﻟﯽ ﮔﯿﺎه ﺧﺮﻓﻪ ﺑﺎ ﺗﻨﻈﯿﻢ ﮐﻨﻨﺪه ﻫﺎي رﺷﺪ ﮔﯿﺎﻫﯽ ﻣﺨﺘﻠﻒ در ﻣﺤﯿﻂ ﮐﺸﺖ MS ﺣﺎوي ﻏﻠﻈﺖﻫﺎي 1 ،0/5 و 2 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ D-0/5 ،2,4 و 1 ﻣﯿﻠﯽﮔﺮم در ﻟﯿﺘﺮ BAP و 0/2 و 0/5 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ Kin ﺣﺎوي ﻏﻠﻈﺖ ﻫﺎي 20 و 30 ﮔﺮم در ﻟﯿﺘﺮ ﺳﺎﮐﺎرز ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. ﺑﺮ اﺳﺎس ﻧﺘﺎﯾﺞ ﺑﻪ دﺳﺖ آﻣﺪه، ﻫﺮ دو رﯾﺰ ﻧﻤﻮﻧﻪ ﻣﻮرد اﺳﺘﻔﺎده داراي 100 درﺻﺪ ﮐﺎﻟﻮس زاﯾﯽ ﺑﻮدﻧﺪ وﻟﯽ ﻋﻤﻠﮑﺮد ﮐﺎﻟﻮس رﯾﺰﻧﻤﻮﻧﻪ ﻫﯿﭙﻮﮐﻮﺗﯿﻞ ﺑﻪ ﺻﻮرت ﻣﻌﻨﯽداري ﺑﯿﺸﺘﺮ از رﯾﺰﻧﻤﻮﻧﻪ ﺑﺮگ ﺑﻮد. ﻣﺤﯿﻂﮐﺸﺖﻫﺎي MS ﺣﺎوي 2 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﻟﯿﺘﺮ BAP و 0/5 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ D-2,4، 2 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ BAP و 1 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ D-1 ،2,4 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﻟﯿﺘﺮ BAP و 1 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ D-1 ،2,4 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ BAP و 0/5 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ D-0/5 ،2,4 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﻟﯿﺘﺮ BAP و 0/5 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﻟﯿﺘﺮ D-2,4 و 1 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ BAP و 2 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ D-2,4، ﻫﻤﮕﯽ ﺣﺎوي 150 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ اﺳﯿﺪآﺳﮑﻮرﺑﯿﮏ در ﻫﺮ دو رﯾﺰﻧﻤﻮﻧﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻋﻤﻠﮑﺮد )وزن ﺗﺮ ﮐﺎﻟﻮس( را داﺷﺘﻨﺪ. در ﮐﺸﺖ ﺳﻮﺳﭙﺎﻧﺴﯿﻮن ﺳﻠﻮﻟﯽ، ﺑﯿﻦ ﺗﯿﻤﺎر ﺑﺎ ﺗﺮﮐﯿﺐ ﺗﻨﻈﯿﻢ ﮐﻨﻨﺪه ﻫﺎي رﺷﺪ ﮔﯿﺎﻫﯽ اﻋﻤﺎل ﺷﺪه از ﻧﻈﺮ ﺷﺎﺧﺺ ﻫﺎي ﺗﻌﺪاد ﺳﻠﻮل در ﯾﮏ ﻣﯿﻠﯽﻟﯿﺘﺮ، PCV ،SCV و ﻣﺪت زﻣﺎن اﻋﻤﺎل ﺗﯿﻤﺎر اﺧﺘﻼف ﻣﻌﻨﯽداري ﻣﺸﺎﻫﺪه ﺷﺪ. ﺑﯿﺸﺘﺮﯾﻦ ﺗﻌﺪاد ﺳﻠﻮل،SCV و PCVدر ﻣﺤﯿﻂ ﮐﺸﺖ MS ﺣﺎوي 2 ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﻟﯿﺘﺮ D-2,4 و 0/2 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ Kin ﺣﺎوي 30 ﮔﺮم ﺑﺮ ﻟﯿﺘﺮ ﺳﺎﮐﺎرز ﻣﺸﺎﻫﺪه ﺷﺪ. ﻃﯽ 32 روز اﻧﺪازهﮔﯿﺮي، ﺑﯿﺸﺘﺮﯾﻦ ﺗﻌﺪاد ﺳﻠﻮل و SCV از روز 26 ام ﺗﺎ 32 ام و ﺑﯿﺸﺘﺮﯾﻦ PCV در روز 26 ام ﻣﺸﺎﻫﺪه ﺷﺪ
چكيده لاتين :
In this research, the effect of type and concentration of BAP and 2,4-D plant growth regulators on callogenesis of hypocotyl and leaf explants of Portulaca oleracea in MS medium containing 0.5, 1 and 2 mg/L of 2,4-D and BAP and optimization of cell suspension culture with different plant growth regulators in MS medium containing 0.5, 1 and 2 mg/L 2,4-D, 0.5 and 1 mg/L BAP, 0.2 and 0.5 mg/L Kin each one containing 20 and 30 g/l sucrose concentration, were investigated. Based on the results, both of the samples had 100 percentage callus induction, but the function of hypocotyl callus was significantly higher than the leaf callus. MS culture media containing 2 mg/l BAP and 0.5 mg/l 2,4-D, 2 mg/l BAP and 1 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l 2,4-D, 1 mg/l BAP and 0.5 mg/l 2,4-D, 0.5 mg/l BAP and 0.5 mg/l 2,4-D and 1 mg/l BAP and 2 mg/l 2,4-D, all containing 150 mg/l ascorbic acid in both explants have the highest yield (wet weight of callus). In the cell suspension culture, there was a significant difference between treatments with the plant growth regulators in terms of number of cells in per ml, SCV, PCV and treatment duration.
