تمايز سلولهاي گرانولوزاي فوليكول تخمدان انساني به كراتينوسايت

DIFFERENTIATION OF HUMAN OVARIAN FOLLICULAR GRANULOSA CELLS INTO KERATINOCYTES

ﭘﻴﺶﺯﻣﻴﻨﻪ ﻭ ﻫﺪﻑ: ﺳﻠﻮﻝﻫﺎﻱ ﺑﻨﻴﺎﺩﻱ ﺳﻠﻮﻝﻫﺎﻱ ﺗﻤﺎﻳﺰ ﻧﻴﺎﻓﺘﻪﺍﻱ ﻣﻲﺑﺎﺷﻨﺪ ﮐﻪ ﺩﺭ ﺑﺎﻓﺖﻫﺎﻱ ﻣﺨﺘﻠﻒ ﻳﺎﻓﺖ ﻣﻲﺷﻮﻧﺪ. ﺍﻳﻦ ﺳﻠﻮﻝﻫﺎ ﺩﺍﺭﺍﻱ ﺧﺼﻮﺻﻴﺖ ﺗﻘﺴﻴﻢ ﻭ ﺗﻤﺎﻳﺰ ﺑﻪ ﺍﻧﻮﺍﻉ ﺭﺩﻩﻫﺎﻱ ﺳﻠﻮﻟﻲ ﻫﺴﺘﻨﺪ. ﺳﻠﻮﻝﻫﺎﻱ ﮔﺮﺍﻧﻮﻟﻮﺯﺍ ﺳﻠﻮﻝﻫﺎﻱ ﺑﻨﻴﺎﺩﻱ ﭼﻨﺪ ﺗﻮﺍﻥ ﻣﻲﺑﺎﺷﻨﺪ. ﺩﺭ ﺍﻳﻦ ﭘﮋﻭﻫﺶ ﺗﻮﺍﻥ ﺗﻤﺎﻳﺰﻱ ﺳﻠﻮﻝﻫﺎﻱ ﺑﻨﻴﺎﺩﻱ ﮔﺮﺍﻧﻮﻟﻮﺯﺍ ﺑﻪ ﺳﻠﻮﻝﻫﺎﻱ ﮐﺮﺍﺗﻴﻨﻮﺳﺎﻳﺖ ﻣﻮﺭﺩ ﺍﺭﺯﻳﺎﺑﻲ ﻗﺮﺍﺭ ﮔﺮﻓﺖ. ﻣﻮﺍﺩ ﻭ ﺭﻭﺵﻫﺎ: ﺳﻠﻮﻝﻫﺎﻱ ﮔﺮﺍﻧﻮﻟﻮﺯﺍ ﺑﻌﺪ ﺍﺯ ﺟﺪﺍﺳﺎﺯﻱ ﺁﻧﺰﻳﻤﻲ ﺍﺯ ﻓﻮﻟﻴﮑﻮﻝ ﺗﺨﻤﺪﺍﻥ ﮐﺸﺖ ﺩﺍﺩﻩ ﺷﺪ، ﺳﭙﺲ ﻣﺤﻴﻂ ﺍﻟﻘﺎﮐﻨﻨﺪﻩﻱ ﮐﺮﺍﺗﻴﻨﻮﺳﺎﻳﺘﻲ ﺑﻪ ﻣﺤﻴﻂ ﮐﺸﺖ ﺍﻭﻟﻴﻪ ﺍﺿﺎﻓﻪ ﮔﺮﺩﻳﺪ ﻭ ﺑﻴﺎﻥ ﮐﺮﺍﺗﻴﻦ 10 ﻭ ﮐﺮﺍﺗﻴﻦ 14 ﺑﻪﻋﻨﻮﺍﻥ ﻣﺎﺭﮐﺮﻫﺎﻱ ﺑﻴﺎﻥ ﮐﺮﺍﺗﻴﻨﻮﺳﺎﻳﺘﻲ ﺗﻮﺳﻂ ﺗﮑﻨﻴﮏ ﻭﺳﺘﺮﻥ ﺑﻼﺕ ﻣﻮﺭﺩﺑﺮﺭﺳﻲ ﻗﺮﺍﺭ ﮔﺮﻓﺖ. ﻳﺎﻓﺘﻪﻫﺎ: ﻧﺘﺎﻳﺞ ﺁﻧﺎﻟﻴﺰ ﻓﻠﻮﺳﺎﻳﺘﻮﻣﺘﺮﻱ ﺳﻠﻮﻝﻫﺎﻱ ﺍﺳﺘﺨﺮﺍﺝﺷﺪﻩ ﻧﺸﺎﻥﺩﻫﻨﺪﻩ ﺑﻴﺎﻥ ﺑﺎﻻﻱ ﺁﻧﺘﻲﮊﻥﻫﺎﻱ ﺍﺧﺘﺼﺎﺻﻲ ﺳﻠﻮﻝﻫﺎﻱ ﺑﻨﻴﺎﺩﻱ ﻣﺰﺍﻧﺸﻴﻤﻲ ﺑﻮﺩ )0/05

Background & Aims: Stem cells are undifferentiated cells and are found in different tissues. These cells have capacity of self-renewal and differentiation into other lineages. Granulosa cells (GCs) are the multipotent stem cells. In the present research we evaluated the differentiation potential of GCs into keratinocytes. Material & Methods: GCs were cultured after enzymatic isolation from ovarian follicle. Then, keratinocyte inductive medium was added and expression of keratin10 and keratin14 were investigated with western blotting technique. Results: The results of the flow cytometric analysis of the isolated cells indicated the high expression of mesenchymal stem cell specific antigens (p < 0.05). Also, the results of the western blotting showed the expression of creatine 10 and creatine 14 proteins in all groups except for negative control (p< 0.05). Conclusion: Human granulosa cells have a very high ability to differentiate into keratinocytic cells, and with further research, it is possible to provide a suitable substrate for the use of human granulosa cells to treat severe skin lesions.