ﻣﻄﺎﻟﻌﻪ ﻣﻘﺎﻭﻣﺖ ﻧﻤﺎﺗﻮﺩﻫﺎﻱ ﺩﺳﺘﮕﺎﻩ ﮔﻮﺍﺭﺵ ﺑﻪ ﺁﻟﺒﻨﺪﺍﺯﻭﻝ ﻭ ﻓﻨﺒﻨﺪﺍﺯﻭﻝ ﺩﺭ ﺷﺮﺍﻳﻂ ﺁﺯﻣﺎﻳﺸﮕﺎﻫﻲ

IN-VITRO ASSESSMENT OF RESISTANCE TO ALBENDAZOLE an‎d FENBENDAZOLE IN GASTROINTESTINAL NEMATODES

ﺗﺎرﯾﺦ درﯾﺎﻓﺖ 1399/05/31 ﺗﺎرﯾﺦ ﭘﺬﯾﺮش 1399/10/08 ﭼﮑﻴﺪﻩ ﭘﻴﺶﺯﻣﻴﻨﻪ ﻭ ﻫﺪﻑ: ﻣﻘﺎﻭﻣﺖ ﺩﺍﺭﻭﻳﻲ ﻳﮑﻲ ﺍﺯ ﺑﺰﺭﮒﺗﺮﻳﻦ ﻣﺸﮑﻼﺕ ﺻﻨﻌﺖ ﮔﻮﺳﻔﻨﺪﺩﺍﺭﻱ ﺩﺭ ﺟﻬﺎﻥ ﻭ ﺍﻳﺮﺍﻥ ﺍﺳﺖ. ﻫﺪﻑ ﺍﺯ ﺍﻳﻦ ﻣﻄﺎﻟﻌﻪ ﺍﺭﺯﻳﺎﺑﻲ ﻣﻘﺎﻭﻣﺖ ﺩﺍﺭﻭﻳﻲ ﻧﺴﺒﺖ ﺑﻪ ﺁﻟﺒﻨﺪﺍﺯﻭﻝ ﻭ ﻓﻨﺒﻨﺪﺍﺯﻭﻝ ﺩﺭ ﻧﻤﺎﺗﻮﺩﻫﺎﻱ ﺩﺳﺘﮕﺎﻩ ﮔﻮﺍﺭﺵ ﮔﻮﺳﻔﻨﺪ ﺑﻮﺩ. ﻣﻮﺍﺩ ﻭ ﺭﻭﺵ ﮐﺎﺭ: ﺩﺭ ﺁﺯﻣﻮﻥ ﺑﺮﻭﻥ ﺗﻨﻲ ﻣﺪﻓﻮﻉ ﺗﺎﺯﻩ ﺍﺯ ﺭﺍﺳﺖ ﺭﻭﺩﻩ 90 ﮔﻮﺳﻔﻨﺪ ﺑﺎ ﺷﺪﺕ ﺁﻟﻮﺩﮔﻲ 150 ﺗﺨﻢ ﻳﺎ ﺑﻴﺸﺘﺮ ﺩﺭ ﮔﺮﻡ ﻣﺪﻓﻮﻉ ﺗﻬﻴﻪ ﺷﺪ. 2ml ﻣﻴﻠﻲﻟﻴﺘﺮ ﻣﺤﻠﻮﻝ ﺣﺎﻭﻱ 1000 ﻋﺪﺩ ﺗﺨﻢ ﺑﻪ ﭼﺎﻫﮏﻫﺎﻱ ﮔﺮﻭﻩﻫﺎﻱ ﺷﺎﻫﺪ، ﺁﻟﺒﻨﺪﺍﺯﻭﻝ )µg /ml 0/1( ﻭ ﻓﻨﺒﻨﺪﺍﺯﻭﻝ )µg /ml 0/1( ﺍﺿﺎﻓﻪ ﺷﺪ ﻭ ﺍﻧﮑﻮﺑﻪ ﮔﺮﺩﻳﺪﻧﺪ. ﺑﺮ ﺍﺳﺎﺱ ﺩﺭﺻﺪ ﺗﺨﻢﻫﺎﻱ ﺗﻔﺮﻳﺦ ﺷﺪﻩ ﻭ ﺷﻤﺎﺭﺵ ﻧﻮﺯﺍﺩﻫﺎﻱ ﻣﺮﺣﻠﻪ ﺍﻭﻝ، ﺩﻭﺯ ﮐﺸﻨﺪﻩ 50ﺩﺭﺻﺪ ﻣﺤﺎﺳﺒﻪ ﻭ ﻣﻴﺰﺍﻥ ﻣﻘﺎﻭﻣﺖ ﺩﺍﺭﻭﻳﻲ ﺗﻌﻴﻴﻦ ﺷﺪ. ﻳﺎﻓﺘﻪﻫﺎ: ﻣﻴﺎﻧﮕﻴﻦ ﺗﻌﺪﺍﺩ ﺗﺨﻢ ﺗﻔﺮﻳﺦ ﺷﺪﻩ )43/76±1/73( ﺩﺭ ﺗﻴﻤﺎﺭﻫﺎﻱ ﻓﻨﺒﻨﺪﺍﺯﻭﻝ ﮐﻤﺘﺮ ﺍﺯ ﺗﻴﻤﺎﺭﻫﺎﻱ ﺁﻟﺒﻨﺪﺍﺯﻭﻝ )65/51±1/4( ﻭ ﮔﺮﻭﻩ ﺷﺎﻫﺪ )93/96±0/76( ﺑﻮﺩ. ﺍﺧﺘﻼﻑ ﻣﻴﺎﻧﮕﻴﻦ ﺗﻌﺪﺍﺩ ﺗﺨﻢ ﺗﻔﺮﻳﺦ ﺷﺪﻩ ﺑﻴﻦ ﺗﻴﻤﺎﺭﻫﺎﻱ ﺁﻟﺒﻨﺪﺍﺯﻭﻝ، ﻓﻨﺒﻨﺪﺍﺯﻭﻝ ﻭ ﮔﺮﻭﻩ ﺷﺎﻫﺪ ﻣﻌﻨﻲﺩﺍﺭﻱ ﺑﻮﺩ. ﻣﻴﺰﺍﻥ ﺩﻭﺯ ﮐﺸﻨﺪﻩ 50ﺩﺭﺻﺪ ﺩﺭ ﮔﺮﻭﻩ ﺗﻴﻤﺎﺭ ﺁﻟﺒﻨﺪﺍﺯﻭﻝ ﺑﻴﺎﻧﮕﺮ ﻭﺟﻮﺩ ﻣﻘﺎﻭﻣﺖ ﺩﺍﺭﻭﻳﻲ ﺑﻮﺩ. ﺩﺭﺣﺎﻟﻲﮐﻪ ﺩﻭﺯ ﮐﺸﻨﺪﻩ 50ﺩﺭﺻﺪ ﺩﺭ ﮔﺮﻭﻩ ﺗﻴﻤﺎﺭ ﻓﻨﺒﻨﺪﺍﺯﻭﻝ ﻧﺸﺎﻧﮕﺮ ﻣﺸﮑﻮﮎ ﺑﻮﺩﻥ ﻣﻘﺎﻭﻣﺖ ﺩﺍﺭﻭﻳﻲ ﺑﻮﺩ. ﺑﺤﺚ ﻭ ﻧﺘﻴﺠﻪﮔﻴﺮﻱ: ﻧﺘﺎﻳﺞ ﺁﺯﻣﻮﻥ ﺑﺮﻭﻥ ﺗﻨﻲ ﺑﻴﺎﻧﮕﺮ ﻭﺟﻮﺩ ﻣﻘﺎﻭﻣﺖ ﺩﺍﺭﻭﻳﻲ ﻧﺴﺒﺖ ﺑﻪ ﺁﻟﺒﻨﺪﺍﺯﻭﻝ ﻭ ﺩﺭ ﺁﺳﺘﺎﻧﻪ ﺑﺮﻭﺯ ﻧﺴﺒﺖ ﺑﻪ ﻓﻨﺒﻨﺪﺍﺯﻭﻝ ﺩﺭ ﮔﻮﺳﻔﻨﺪﺍﻥ ﺗﺤﺖ ﻣﻄﺎﻟﻌﻪ ﺑﻮﺩ.

Background & Aims: Drug resistance is a great concern worldwide and in Iran. This study was carried out to assess drug resistance of gastrointestinal nematodes to Albendazole (Alb) and Fenbendazole (Feb) in sheep. Materials & Methods: A total number of 90 fresh fecal samples were directly collected from the rectum of infected sheep with an average egg per gram of feces (EPG)≤150. A dilution of 1000 eggs per 2mL was added to each well of control, Alb (0.1µg/ml) and Feb (0.1µg/ml) groups and incubated. To determine drug resistance, a lethal dose of 50% (LD50) was calculated based on percentage of the hatched eggs and first larvae stage (L1) counting. Results: EPG was lower in Feb treated groups (43.76±1.73) than Alb treated groups (65.51±1.4) and control group (93.96±0.76). There was a significant difference between percentage of the hatched eggs and both treated and control groups. LC50 demonstrated resistance to Alb in treated groups; while it uncovered suspicion to drug resistance in Feb treated groups. Conclusion: It was concluded that there was resistance to Alb and suspected resistance to Feb in sheep examined.