تاثير تمرين هوازي و مصرف ويتامين Dهمراه با تزريق سلول هاي بنيادي مشتق از بافت چربي بر شاخص هاي آپوپتوزي و ضد آپوپتوزي سلول هاي بتاي پانكراس رت هاي ديابتي

Effect of Aerobic Training, Vitamin D, and Injection of Adipose Tissue-Derived Stem Cells on Some Apoptotic and Anti- Apoptotic Indices of Beta-Pancreatic Cells in Diabetic Rats

سابقه و هدف: آپوپتوز فرآيند سلولي محافظتكننده است كه نقش مهمي در توسعه و هومئوستاز بافت طبيعي و نيز به همراه تزريق سلولهاي D پيدايش بيماريها دارد. مطالعه حاضر با هدف تعيين تأثير تمرين هوازي ومصرف ويتامين بنيادي مشتق از بافت چربي بر برخي از شاخصهاي آپوپتوزي وضدآپوپتوزي سلولهاي بتاي پانكراس رتهاي ديابتي انجام شد. (STZ) شده با استرپتوزوسين 220 گرم وسن 8 هفته) به 10 گروه كنترل، شم، - ﻮاد و روشﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ،80 ﺳــﺮ رت)ﺑــﺎ وزن 220-240ﮔــﺮم وﺳــﻦ 8 ﻫﻔﺘــﻪ( ﺑــﻪ 10ﮔــﺮوه ﮐﻨﺘــﺮل، ﺷــﻢ، دﯾﺎﺑﺖ، ﺗﻤﺮﯾﻦ، ﺳﻠﻮل، وﯾﺘﺎﻣﯿﻦ، ﺗﻤﺮﯾﻦ و ﺳﻠﻮل، ﺗﻤﺮﯾﻦ و وﯾﺘﺎﻣﯿﻦ، ﺳﻠﻮل و وﯾﺘﺎﻣﯿﻦ، ﺗﻤﺮﯾﻦ و ﺳﻠﻮل و وﯾﺘﺎﻣﯿﻦ ﺗﻘﺴــﯿﻢ ﺷــﺪﻧﺪ. ﮔﺮوه ﺗﻤﺮﯾﻨﯽ 6 ﻫﻔﺘﻪ و ﻫﻔﺘﻪاي ﭘﻨﺞ روز ﺑﺎ ﺷﺪت 60-70 درﺻﺪ VO2max ﺑــﻪ ﺗﻤــﺮﯾﻦ روي ﻧــﻮارﮔﺮدان ﭘﺮداﺧﺘﻨــﺪ. در ﮔــﺮوه ﺳﻠﻮل ﺑﻪ ﺳﯿﺎﻫﺮگ دم ﻣﻮشﻫﺎي دﯾﺎﺑﺘﯽ، ﺑﻪ ﮐﻤﮏ ﺳﺮﻧﮓ اﻧﺴﻮﻟﯿﻨﯽ PBS/5ml ﺣﺎوي 1/5×106ﻋﺪد ﺳﻠﻮل اﺳــﺘﺨﺮاج ﺷــﺪه از ﺑﺎﻓﺖ ﭼﺮﺑﯽ اﻧﺴﺎﻧﯽ ﺗﺰرﯾﻖ ﺷﺪ. ﺑﺮاي اﯾﺠﺎد ﻣﺪل دﯾﺎﺑــﺖ، STZ ﺑــﺎ دوز 60mg/kg درﺗﺮﮐﯿــﺐ ﺑــﺎ ﺑﺎﻓﺮﺳــﯿﺘﺮات و PH 4/5 درون ﺻﻔﺎق ﺗﺰرﯾﻖ ﺷﺪ. ﻣﻘﺎدﯾﺮ Bax و Bcl2 ﺳﻠﻮلﻫﺎي ﺑﺘﺎي ﭘﺎﻧﮑﺮاس، ﺑﻌﺪ از ﻫﻤﻮژﻧﯿﺰه وﺳﺎﻧﺘﺮﯾﻔﯿﻮژ ﺑﻪوﺳﯿﻠﻪ ﮐﯿﺖ آزﻣﺎﯾﺸﮕﺎﻫﯽ ﺑــﺎ روش اﻻﯾﺰا اﻧﺪازهﮔﯿﺮي ﺷﺪ. دادهﻫﺎ ﺑﺎ آزﻣﻮن وارﯾﺎﻧﺲ ﯾﮏﻃﺮﻓﻪ و ﺗﻌﻘﯿﺒﯽ ﺗﻮﮐﯽ درﺳﻄﺢ 0/05≤P آﻧﺎﻟﯿﺰ ﺷﺪ ﯾﺎﻓﺘﻪﻫﺎ: ﻧﺘﺎﯾﺞ ﻧﺸــﺎن داد ﺑــﯿﻦ ﺳــﻄﻮح Bax و Bcl2 و ﻧﺴــﺒﺖ Bax/Bcl2 اﺧــﺘﻼف ﻣﻌﻨــﯽداري وﺟــﻮد دارد)0/001=P(. ﺑﺮاﺳﺎس ﻧﺘﺎﯾﺞ آزﻣﻮن ﺗﻌﻘﯿﺒﯽ ﺗﻮﮐﯽ اﯾﻦ اﺧﺘﻼف ﺑﯿﻦ ﮔﺮوه دﯾﺎﺑﺖ ﺑﺎ ﮐﻨﺘﺮل و دﯾﺎﺑﺖ ﺑﺎ ﺗﻤﺎﻣﯽﮔﺮوهﻫﺎ ﺑﻮده وﻟﯽ ﺑﯿﻦ ﺳﺎﯾﺮ ﮔﺮوهﻫﺎ ﺗﻔﺎوت ﻣﻌﻨﯽداري دﯾﺪه ﻧﺸﺪ)0/001=P(. استنتاج: به نظر ميرسد هر كدام از مداخلات، مخصوصاتركيب آنها باعث تاثيرافزايشي بر روي مقادير 2 سلولهاي بتاي پانكراس داشته باشد و ميتواند بهعنوان يك روش غيردارويي Bcl-2/Bax و نسبت Bax كاهش مقادير براي كاهش عوارض آپوپتوز ناشي از ديابت استفاده شود.

Apoptosis is a protective cellular process that plays an important role in the development and homeostasis of natural tissues as well as disease-causing factors. Current study aimed at determining the effect of aerobic training, Vitamin D, and injection of adipose tissuederived stem cells (ADSCs) on some apoptotic and anti-apoptotic indices of beta-pancreatic cells of streptozotocin (STZ) induced diabetic rats. Materials and methods: In an experimental study, 8orats (220-240g, 8 weeks old) were randomly divided into 10 groups: Control, Sham, diabetics, ADSCs, exercise, Vitamin D, ADSCs +exercise, ADSCs +Vitamin D, exercise+ Vitamin D, and ADSCs +exercise+ Vitamin D. The exercise protocol included treadmill running at 60-70% VO2max for 5 days a week/ 6 weeks. In ADSCs receiver groups, human ADSCs (1.5*106 PBS/5mL) were injected to the tail vein. Diabetes was induced by intraperitoneal injection of streptozotocin (60mg/kg) dissolved in citrate buffer, pH 4.5. Bax and Bcl2 values of beta pancreatic cells were measured following homogenization and centrifugation using ELISA. Data analysis was done applying one way analysis of variance and Tukey test. Results: Significant differences were seen between the levels of Bax and Bcl2a well as the ratio of Bcl-2/Bax between diabetic group and control group and diabetic group and other groups (P=0.001). But, there were no significant differences between other groups (P=0.001). Conclusion: It seems that all interventions, especially their combination, can have an increasing effect on Bcl2 values and decrease Bax values and the ratio of beta-pancreatic cells. So, they can be used as a Bax pharmacological method to reduce hyperglycemia-induced apoptosis on beta cells