تاثير 6 هفته تمرين استقامتي و عصاره تاكسول بر VEGF و ترومبواسپاندين در بافت كليه موش هاي C57 مبتلا به سرطان گردن رحم

The effect of 6-Week Endurance training and Taxol extract on kidney tissue VEGF and Thrombospondin in C57 Mice With Cervical Cancer

زمينه و هدف هدف از اين پژوهش تاثير شش هفته تمرين استقامتي و عصاره تاكسول بر VEGF و ترومبواسپاندين در بافت كليه موش هاي C57 مبتلا به سرطان گردنه رحم بود. روش كار در اين تحقيق تجربي، تعداد 35 سر موش ماده C57 با سن هشت هفته، به طور تصادفي به پنج گروه كنترل (سالم)، كنترل (سرطان)، عصاره تاكسول، تمرين (سرطان) و عصاره تاكسول -تمرين (سرطان) با هفت سر موش در هر گروه تقسيم شدند. كاشت تومورهاي سرطاني در زير پوست بخش فوقاني لگن انجام شد. گروه تمرين، پروتكل تمريني استقامتي شامل سه جلسه در هفته، 50 دقيقه در هر جلسه با سرعت 18-14 متر بر ثانيه به مدت شش هفته را اجرا كردند. مقدار 60 ميلي گرم/كيلوگرم/روز ، عصاره خالص تاكسول به صورت زير صفاقي تزريق شد. جهت تجزيه و تحليل داده ها از تحليل واريانس يك طرفه و آزمون تعقيبي بونفروني در سطح 05/0>p استفاده شد. يافته ها نتايج نشان داد عصاره تاكسول و تمرينات استقامتي سطوح VEGF بافت كليه موش هاي C57 مبتلا به سرطان گردن رحم را به طور معني داري كاهش مي دهد 0/000 =p). همچنين عصاره تاكسول و تمرينات استقامتي سطوح ترومبواسپاندين بافت كليه موش هاي C57 مبتلا به سرطان گردن رحم را به طور معني داري افزايش داد (0/000 =p). نتيجه گيري با توجه به يافته هاي تحقيق حاضر به نظر مي رسد، تمرينات استقامتي هوازي و عصاره تاكسول احتمالا مي توانند مداخلات موثري براي كاهش پيش روي سرطان گردن رحم باشند.

Cervical cancer is one of the most common cancers in women. Genital carcinomas, especially cervical cancer, make up almost 12% of all cancers in women and are the second most common female genital cancer in the world. Cervical cancer is caused by specific papillomavirus (HPV) infections. Various factors, including regular exercise, reduce the risk of cancer. An active lifestyle can reduce the risk of developing cancer. Studies show that active women are 20 to 30 percent less likely to develop cancer than inactive women. Clinical evidence suggests that angiogenesis plays an important role in cervical cancer. Specifically, overexpression of VEGF is associated with unpleasant oncological consequences in cervical cancer tumors. VEGF is an important growth factor involved in neoangiogenesis and is expressed in glomerular pedocytes and renal endothelial cells. Also, Thrombospandine-1 (TSP1) inhibits tumor growth and metastasis and is a potent inhibitor of angiogenesis and tumorigenesis in vivo. Taxol is a compound of diterpene with a complex structure that is often extracted from the yew plant. This substance is currently the most important natural anti-cancer compound with a different mechanism from other similar drugs in this field, worldwide for the treatment of various cancers such as skin, lung, urinary tract, esophagus, lymph nodes and ... It is used effectively. To date, no study has examined the response of VEGF and thrombospandine-1 to aerobic exercise in patients with cervical cancer. On the other hand, today there is an increasing tendency to use plants and their compounds as potential anti-cancer drugs that have a direct toxic activity on malignant cells. The results of the present study can help to better explain the factors involved in cervical cancer; Therefore, the aim of this study was to investigate the effect of 6-Week Endurance training and Taxol extract on kidney tissue VEGF and Thrombospondin in C57 Mice with Cervical Cancer. Methods In this experimental study, 35 female C57 mice, eight weeks old, were randomly divided into 5 groups: control (healthy), control(cancer), Taxol extract, training(cancer), and Training-Taxol extract (cancer) with 7 mice in each group. The implantation of cancerous tumors was performed under the skin of the upper pelvis. The training group completed the endurance training protocol, which included 3 sessions per week, 50 minutes per session, at a speed of 14-18 m/s for six weeks. A dose of 60 mg/kg/day, a pure extract of Taxol was injected peritoneally Data were analyzed by t-test, One-way ANOVA and post hoc Bonferronchr('39')s at the significant level p<0.05. Results The results showed that the use of taxol extract and endurance exercises significantly reduced the levels of VEGF in kidney tissues of C57 mice with cervical cancer (p=0.000). Also, the use of taxol extract and endurance exercises significantly reduced the levels of thrombospondin in kidney tissues of C57 mice with cervical cancer (p=0.000). Conclusion The findings of this study were consistent with the results of Previous research [19,20,25]. VEGF in response to exercise is due to the binding of VEGF to receptors on endothelial cells, which is a stimulus for the angiogenesis process. Also, Decreased VEGF can be due to binding to other proteins, including heparin sulfate and endothelial stem cells. It is possible that one of the reasons for the decrease in VEGF in response to the exercise is due to the increase of VEGF production inhibitors, namely ANP and CNP. Another reason for the decrease in VEGF to exercise can be explained from the perspective of somatostatin. Taxol is a diterpene compound with a complex structure. It is rich in isoflavones. Flavonoids such as 3,4 dihydroxyflavones, 1,3 dihydroxyflavones, Fisetin, Apigenin and Lutelin are able to inhibit angiogenesis in vitro in the micromolar concentration range. The results of studies have shown that taxol inhibits angiogenesis at low concentrations of picomolar by inhibiting the production of VEGF and inhibiting the protein expression of alpha factor induced by hypoxia. There were some limitations in the present study, such as the lack of measurement of other angiogenic factors and anti-angiogenic factors. According to the findings of this study, aerobic endurance training and Taxol extract may be effective interventions to reduce the progression of cervical cancer.