كليدواژه :
تمرين تناوبي شديد , سندرم متابوليك , رزيستين , PAI-1 , PAI-1
چكيده فارسي :
هدف از اين پژوهش، بررسي تاثير 6 هفته تمرين تناوبي شديد بر بيان ژن PAI-1 و رزيستين چربي احشايي در رتهاي مبتلا به سندرم متابوليك بود.
روش كار: در اين تحقيق تجربي، تعداد 32 سر رت نر نژاد ويستار با وزن 20±180 گرم انتخاب و پس از 12 هفته رژيم غذايي پرچرب و ايجاد مدل سندرم متابوليك به طور تصادفي در 4 گروه كنترل، سندرم متابوليك، تمرين تناوبي شديد و سندرم متابوليك + تمرين تناوبي شديد قرار گرفتند. برنامه تمرين تناوبي شامل 5 تا 10 تناوب 1 دقيقه اي دويدن شديد بر روي تردميل با شدت 80 تا 95 درصد حداكثر سرعت و در تناوب هاي آهسته با سرعت 55 درصد حداكثر سرعت با شيب صفر تردميل به مدت 6 هفته اجرا گرديد. دادهها با استفاده از آزمون تحليل واريانس يك طرفه و آزمون تعقيبي توكي در سطح معنيداري 0/05 p< تجزيه و تحليل شد.
يافته ها: نتايج نشان داد كه سطوح بيان ژن PAI-1 و رزيستين در موشهاي مبتلا به سندرم متابوليك نسبت به ساير گروهها بيشتر بود. شش هفته تمرين تناوبي شديد موجب كاهش معني دار بيان ژن رزيستين و PAI-1 شد (0/001 p=).
نتيجه گيري: با توجه به نتايج، به نظر ميرسد كه چاق شدن و افزايش بافت چربي احشايي ميتواند ميزان بيان ژن رزيستين و PAI-1 را افزايش داده و تمرينات تناوبي شديد ميزان آن را كاهش دهد
چكيده لاتين :
Sedentary lifestyle is associated with unhealthy health consequences, especially overweight and obesity, and obesity is associated with an increased risk of metabolic syndrome (1). Numerous studies have shown that visceral adipose tissue plays an important role in the development of metabolic syndrome (4, 5). Uncontrolled production of adipocytokines is involved in the pathogenesis of obesity-related metabolic syndrome (7).
Studies showed that regular physical activity is a good way to reduce the symptoms of metabolic syndrome (18, 19). high-intensity interval training has been reported to burn more calories and increase fat oxidation, as well as more energy expenditure than continuous exercise (steady state) (21). However, the results of studies the effect of HIIT on resistin are contradictory (22, 23). Also following HIIT, were reported decrease (24, 25) and no significant change (26) of PAI-1. There is a lot of ambiguity about the most appropriate training method to control adipokine activity in people with the metabolic syndrome. It is expected that by conducting the present study, while answering some of the ambiguities in determining the effect of high-intensity interval training program on the biochemical process of metabolic disorders, it will be possible to make appropriate suggestions on how to perform exercise and predict possible consequences. Therefore, the present study intends to investigate the effect of 6 weeks high-intensity interval training on the gene expression of PAI-1 and resistin visceral fat in rats with metabolic syndrome.
Methods: This is an experimental study. This research was conducted on Wistar rats in at Baqiyatallah University of Medical Sciences, Tehran, Iran. thirty-two rats (weighing 180± 20 gr) were selected and randomly divided into 4 groups including control, metabolic syndrome, High-Intensity Interval Training and metabolic syndrome + High-intensity interval training. Rats were randomly divided into two groups: standard diet and high fat diet (HFD: High Fat Diet), then next to the group with standard diet, rats in the high fat diet group for 12 weeks. They underwent a special diet (30 to 40% fat) to develop a model of metabolic syndrome, and after 12 weeks, blood samples (fat profile, insulin resistance, fasting glucose) were taken from this group to Confirm of model the disease (27). High-Intensity Interval Training consisted of 5 to 10 interval 1-minute intensive running on the treadmill at 80 to 95% of maximum speed and in slow alternations at 55% of Maximum speed with zero treadmill slope. gene expression of PAI-1 and resistin in visceral fat was measured after training using Real Time PCR.
Results: The results showed that PAI-1 and resistin gene expression levels were higher in rats with metabolic syndrome than the other groups. Six weeks of High-intensity interval training significantly decreased the expression of resistin and PAI-1.
Conclusion: The findings of this study were consistent with the results of Previous research (14, 24, 25). A possible cause of decreased PAI-1 activity in this study could be reduced adipose tissue in rats that performed high-intensity interval training. Also, it is possible that exercise reduces the production of PAI-1 by inhibiting NF-Kb protein transcription. In the present study, possible causes of reduction in resistin were weight loss, fat percentage and the ability of HIIT to upset energy balance. Some researchers have reported decrease in resistin due to decrease in inflammatory cytokines such as IL-6, TNF, and IL-1 (38). It is recommended that high-intensity interval training be considered in the treatment programs of patients with metabolic syndrome. In the present study, there were limitations in the present study, including the study of animal specimens. Other limitations of this study include lack of measurement of other adipokines associated with the pathogenesis of metabolic syndrome. According to the results, it seems that obesity and visceral adipose tissue can increase the rate of resistin and PAI-1 gene expression and decrease high-intensity interval training.
