بررسي اثر هم كشتي سلول هاي بنيادي بر بافت تخمدان موش سوري

Studying the effect of co-culture system of Mesenchymal Stem Cells on development of follicles in mice ovary in vitro

با استفاده از تكنيك هاي كشت سلول و كشت بافت بسياري از محققان براي روشن كردن عوامل هورموني لازم براي تمايز ساختاري و فعاليت هاي عملكردي بافت تخمدان تلاش مي كنند.لذا در اين مطالعه ما به بررسي كشت بافت تخمدان بر روي سلولهاي بنيادي مزانشيمي جداشده از بافت چربي پرداخته ايم.دراين مطالعه سلول هاي بنيادي مزانشيمي از بافت چربي محوطه بطني موش سوري جدا گرديد. پس از پنج روز و تشكيل تك لايه اي از سلول هاي بنيادي مزانشيمي، تخمدان هاي گرفته شده بمدت هفت روز بر روي مونولاير سلول هاي كشت شده مزانشيمي قرار گرفت. داده هاي به دست آمده با استفاده از نرم افزار Spss مورد تجزيه و تحليل قرار گرفت.ﺎﻓﺘﻪ ﻫﺎ: ﻧﺘﺎﯾﺞ ﺑﺪﺳﺖ آﻣﺪه در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن داد ﮐﻪ ﺗﻌﺪاد ﻓﻮﻟﯿﮑﻮل ﻫﺎي ﺑﺎﻟﻎ ﮔﺮوه ﺗﯿﻤﺎر ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل ﺑﻪ ﺗﺮﺗﯿﺐ )61/1±2/2 ،38/9±1/9( ﺑﻮده اﺳﺖ ﮐﻪ در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﮐﻨﺘﺮل اﻓﺰاﯾﺶ ﻣﻌﻨﯽ داري داﺷﺘﻪ اﺳﺖ )0/05

Many researchers are trying to clarify necessary hormonal factors for structural distinguishability and functional activities of ovarian tissue by using cell culture and tissue culture techniques. Hence, in this study, we examined ovarian tissue culture on mesenchymal stem cells isolated from cultured adipose tissue. Materials and methods: In this study, mesenchymal stem cells became isolated from adipose tissue of the mouse. After five days and monolayer formation of Mesenchymal stem cells, obtained ovarian was placed on the cultured Mesenchymal stem cells’ monolayer for 7 days. Results: The results of this study show that numbers of mature follicles for treat group compared to the control group were 61/1±2/2 and 38/1±1/9, respectively which had a significant increase compared to control group (P<0/05). This shows the efficiency of tissue culture in co-culture with Mesenchymal stem cells. The real-time PCR studies also confirmed the decrease in the BAX genes in treatment Groups to compare the control group (P<0/05). According to the results yielded regarding the number and quality of obtained follicles, it seems that this method is efficient and of high importance in producing mature follicles, and subsequently high quality oocytes and embryo.