بهبود شاخص‌هاي ايمني موكوس پوست ماهي كپور معمولي (Cyprinus carpio) در مواجهه با نانو ذره آهن با استفاده از مكمل غذايي ملاس

Improvement of skin mucus immunological indices of common carp )Cyprinus carpio( in exposure to iron nanoparticles using molasses dairy supplement

وجود آلاينده ­هاي نوظهور نانوذرات در آب باعث پاسخ استرس در ماهي مي­شود كه نهايتاً بر وضعيت فيزيولوژيك ماهيان اثر­گذار بوده و باعث كاهش عملكرد ايمني ماهيان مي­شود. از اين رو، استفاده از محرك­هاي ايمني نظير ملاس بسيار ضروري به­نظر مي­رسد. در اين تحقيق، تعداد 250 قطعه بچه­ ماهي كپور معمولي به­ مدت 42 روز در چهار دسته: جيره فاقد ملاس (تيمار 1)، جيره حاوي 0/5 درصد ملاس (تيمار 2)، جيره حاوي 1 درصد ملاس (تيمار 3) و جيره حاوي 2 درصد ملاس (تيمار 4) تقسيم شدند. سپس به هركدام از گروه‌ها 50 درصد غلظت كشنده نانو آهن به­مدت چهارده روز اضافه ‌شد. شاخص­هاي مختلف از جمله : پروتئين محلول، فسفاتاز قليايي، ليزوزيم و ايمونوگلوبولين (IgM) در موكوس اندازه­گيري شد. نتايج تجزيه ­و­تحليل آماري داده­ها نشان داد كه در مجموع تيمارهاي آزمايشي بر شاخص­ هاي ايمني موكوس تأثير معني­داري داشت )0/05

The emergence of newly released nanoparticles in water results in stress response of fishes, which ultimately affects the physiological state and reduces the immune function of fishes. Therefore, it seems the use of immune stimulants such as molasses is very necessary. In this study 250 common carp were exposed to molasses in four groups, with no molasses (treatment 1), diet containing 0.5% molasses (treatment 2), 1% molasses (treatment 3) and diet containing 2% molasses (Treatment 4) for 42 days. Each group were exposed to 50% of lethal concentration of nano-iron for 14 days. Then, various indices such as soluble protein, alkaline phosphatase, lysozyme and immunoglobulin were measured in mucus. Results showed that total experimental treatments had a significant effect on mucus immune parameters (P<0.05), so these indices were affected by treatments fed with molasses increased with the increase of molasses concentration, in the treatment of nano-iron and in the treatment of nano-iron and molasses, the amount of this indicator increased significantly. The overall conclusion of this study shows that molasses, iron nanoparticles, molasses and iron compound increased the mucosal immune parameters.