مقايسه مقاومت آنتي بيوتيكي و فاكتورهاي ويرولانس در جدايه هاي انتروكوكوس فكاليس جدا شده از منابع دامي و انساني در سال 1397

Comparison of antibiotic resistance and virulence factors in Enterococcus faecalis isolates isolated from animal and human sources

اﻧﺘﺮوﮐﻮكﻫﺎ ﮔﺮوه ﻣﻬﻢ و ﻣﺘﻨﻮﻋﯽ از ﺑﺎﮐﺘﺮيﻫﺎ ﻫﺴﺘﻨﺪ ﮐﻪ ﺑﻪﻋﻨﻮان ﺑﺎﮐﺘﺮي ﻣﻘﺎوم ﺑﻪ ﺑﯿﺸﺘﺮ آﻧﺘﯽﺑﯿﻮﺗﯿﮏﻫﺎي ﻣﺼﺮﻓﯽ در درﻣﺎن ﺑﯿﻤﺎريﻫﺎ ﺷﻨﺎﺧﺘﻪ ﺷﺪهاﻧﺪ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻣﻘﻄﻌﯽ- ﺗﻮﺻﯿﻔﯽ 104 ﻧﻤﻮﻧﻪ ﮔﻮﺷﺖ ﻗﺮﻣﺰ و 1000 ﻧﻤﻮﻧﻪ ادرار ﻣﺸﮑﻮك ﺑﻪ ﻋﻔﻮﻧﺖ دﺳﺘﮕﺎه ادراري در ﺷﻬﺮﺳﺘﺎن ﮐﺮﻣﺎﻧﺸﺎه ﺟﻬﺖ ﺑﺮرﺳﯽ اﻧﺘﺮوﮐﻮﮐﻮس ﻓﮑﺎﻟﯿﺲ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﭘﺲ از ﺗﺎﯾﯿﺪ ﻧﻤﻮﻧﻪﻫﺎ ﺑﺎ روشﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ و ﻣﻮﻟﮑﻮﻟﯽ ﺑﻪﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﺗﻮاﻧﺎﯾﯽ ﺗﻮﻟﯿﺪ ﺑﯿﻮﻓﯿﻠﻢ از روش ﻣﯿﮑﺮوﺗﯿﺘﺮ ﭘﻠﯿﺖ و ﺑﺮاي ﺗﻌﯿﯿﻦ ﺣﺴﺎﺳﯿﺖ آنﻫﺎ ﻧﺴﺒﺖ ﺑﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏﻫﺎ، از روش ﮐﺮﺑﯽ- ﺑﺎﯾﺮ اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. در ﻧﻤﻮﻧﻪﻫﺎي اﻧﺴﺎﻧﯽ آﻟﻮدﮔﯽ ﺑﻪ اﻧﺘﺮوﮐﻮﮐﻮس ﻓﮑﺎﻟﯿﺲ 5 درﺻﺪ و در ﻧﻤﻮﻧﻪ ﮔﻮﺷﺖ ﻣﻮرد ﺑﺮرﺳﯽ 40/38 درﺻﺪ ﮔﺰارش ﮔﺮدﯾﺪ. در ﺟﺪاﯾﻪﻫﺎي ﺟﺪا ﺷﺪه از ﮔﻮﺷﺖ ﻗﺮﻣﺰ ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺎوﻣﺖ ﻧﺴﺒﺖ ﺑﻪ اﺳﺘﺮﭘﺘﻮﻣﺎﯾﺴﯿﻦ و ﮐﻤﺘﺮﯾﻦ ﻣﻘﺎوﻣﺖ ﻧﺴﺒﺖ ﺑﻪ وﻧﮑﻮﻣﺎﯾﺴﯿﻦ ﮔﺰارش ﺷﺪ. در ﺟﺪاﯾﻪﻫﺎي اﻧﺴﺎﻧﯽ ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺎوﻣﺖ ﻧﺴﺒﺖ ﺑﻪ ﮐﻮﺗﺮﯾﻤﻮﮐﺴﺎزول و ﮐﻤﺘﺮﯾﻦ ﻣﻘﺎوﻣﺖ ﻧﺴﺒﺖ ﺑﻪ ﻧﯿﺘﺮوﻓﻮراﻧﺘﺌﯿﻦ ﮔﺰارش ﮔﺮدﯾﺪ. در ﺟﺪاﯾﻪﻫﺎي اﻧﺘﺮوﮐﻮﮐﻮس ﻓﮑﺎﻟﯿﺲ ﺟﺪا ﺷﺪه از ﮔﻮﺷﺖ ﻗﺮﻣﺰ، ژن ebp A )71/43 درﺻﺪ(، ebp B )59/52 درﺻﺪ( و ebp C )64/28 درﺻﺪ( ﮔﺰارش ﮔﺮدﯾﺪ ﮐﻪ ارﺗﺒﺎط آﻣﺎري ﻣﻌﻨﺎدار ﺑﯿﻦ ژنﻫﺎي ebp وﺗﻮﻟﯿﺪ ﺑﯿﻮﻓﯿﻠﻢ در اﯾﻦ ﺟﺪاﯾﻪﻫﺎ ﻣﺸﺎﻫﺪه ﻧﮕﺮدﯾﺪ. در ﺻﻮرﺗﯽﮐﻪ در ﺟﺪاﯾﻪ-ﻫﺎي ﺟﺪا ﺷﺪه از ادرار ﺑﯿﻦ ژنﻫﺎي ebp و ﺗﻮﻟﯿﺪ ﺑﯿﻮﻓﯿﻠﻢ ارﺗﺒﺎط ﻣﻌﻨﯽدار ﻣﺸﺎﻫﺪه ﺷﺪ. ﻫﻢﭼﻨﯿﻦ در ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ آﻣﺎري ﺑﯿﻦ ﻧﻮع ﮔﻮﺷﺖ و ﻧﻮع ژن وﯾﺮوﻻﻧﺲ راﺑﻄﻪ ﻣﻌﻨﯽ دار آﻣﺎري ﻣﺸﺎﻫﺪه ﻧﮕﺮدﯾﺪ. در ﺻﻮرﺗﯽﮐﻪ ﺑﯿﻦ ﻓﺮاواﻧﯽ ژنﻫﺎي ace ،gel E ،efa A و esp در ﺟﺪاﯾﻪﻫﺎي اﻧﺘﺮوﮐﻮﮐﻮس ﻓﮑﺎﻟﯿﺲ ﺟﺪا ﺷﺪه از ادرار و ﺗﻮﻟﯿﺪ ﺑﯿﻮﻓﯿﻠﻢ ارﺗﺒﺎط ﻣﻌﻨﯽدار ﻣﺸﺎﻫﺪه ﺷﺪ.

Enterococci are an important and diverse group of bacteria that are known to be resistant to most antibiotics used to treat diseases. In this cross-sectional study, 104 samples of red meat and 1000 urine samples suspected of urinary tract infection in the border city of Kermanshah were examined for Enterococcus faecalis. First, the samples were approved by biochemical and molecular methods, then in order to evaluate their ability to produce biofilm, Microtiter Plate method was used and their sensitivity to antibiotics was also determined by Kirby-Bayer method. Enterococcus faecalis infection in human samples and red meat samples was reported to be 5% and 40.38% respectively. In the strains isolated from red meat samples, the highest resistance was reported to be to Streptomycin while the lowest resistance was to Vancomycin. In the human isolate samples, the highest resistance was reported to be to Co-trimoxazole ,while the lowest resistance was to Nitrofurantoin . In strains isolated from red meat, ebp A, ebp B and ebp C were reported to be 71.43%, 59.52% and 64.28% respectively. No statistically significant relationship was observed between biofilm production and ebp genes in these isolates. However, in strains isolated from urine, a significant relationship was detected between ebp genes and biofilm production. Similarly, it was reported that there was no statistically significant relationship between the meat type and the virulence gene type. But, the findings of the study showed a significant relationship between the frequency of efa A, gel E, ace and esp genes.