ررسي بيان ژن هاي الفا سنتاز و گاما سنتاز و ارتباط آن با بيوسنتز مونوترپن تيمول و كارواكرول در گياه دارويي آويشن باغي رقم واريكو 3

Investigation of γ-Terpinene synthesis and α- Terpinene synthesis reductoisomerase genes expression and its relation to monoterpene carvacrol biosynthesis in Thymus vulgaris cv. ‘Varico 3

آوﯾﺸﻦ ﺑﺎﻏﯽ ).Thymus vulgaris L( ﮔﯿﺎﻫﯽ ﺧﺸﮑﯽ دوﺳﺖ ﺑﻮده و ﺑﻪ ﺳﻬﻮﻟﺖ ﻗﺎدر ﺑﻪ ﺗﺤﻤﻞ ﮐﻢ آﺑﯽ و ﺧﺸﮑﯽ اﺳﺖ، ﻣﻬﻢﺗﺮﯾﻦ ﻣﺎده ﻣﻮﺛﺮه ﻣﻮﺟﻮد در آوﯾﺸﻦ ﺗﯿﻤﻮل اﺳﺖ داراي ﺧﻮاص ﺿﺪ ﻣﯿﮑﺮوﺑﯽ، داروﯾﯽ و آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﻣﯽﺑﺎﺷﺪ. ﺗﺎﮐﻨﻮن ﺗﺤﻘﯿﻘﯽ در راﺑﻄﻪ ﺑﺎ ﺗﺎﺛﯿﺮ ﺳﺮﻣﺎ ﺑﺮ ﻣﯿﺰان ﺗﻐﯿﯿﺮات ژنﻫﺎي ﻣﺴﯿﺮ ﺳﻨﺘﺰ ﺗﯿﻤﻮل در آوﯾﺸﻦ اﻧﺠﺎم ﻧﺸﺪه اﺳﺖ. اﯾﻦ ﭘﮋوﻫﺶ ﺑﻪﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﺗﺎﺛﯿﺮ ﺗﻨﺶ دﻣﺎي ﭘﺎﯾﯿﻦ در زﻣﺎنﻫﺎي ﺻﻔﺮ، 24 ،12 ،3 و 48 ﺳﺎﻋﺖ ﭘﺲ از اﻋﻤﺎل ﺗﯿﻤﺎر در ﻗﺎﻟﺐ ﻃﺮح ﮐﺎﻣﻼ ﺗﺼﺎدﻓﯽ ﺑﯿﺎن ژنﻫﺎي درﮔﯿﺮ در ﺑﯿﻮﺳﻨﺘﺰ ﺗﯿﻤﻮل ﺷﺎﻣﻞ TPS2 وTPS5 ﺑﺎ اﺳﺘﻔﺎده از روش -RT PCR ﻃﯽ ﺳﺎلﻫﺎي 1394 و 1395 اﻧﺠﺎم ﺷﺪ. ﺟﻬﺖ ﺑﺮرﺳﯽ ﻣﯿﺰان ﺑﯿﺎن ﻧﺴﺒﯽ ژنﻫﺎي ﻣﺴﯿﺮ ﺳﻨﺘﺰ ﺗﯿﻤﻮل )MVA , MEP( در آوﯾﺸﻦ ﺑﺎﻏﯽ رﻗﻢ وارﯾﮑﻮ3 ﺗﺤﺖ ﺗﻨﺶ ﺳﺮﻣﺎﯾﯽ از آزﻣﻮن t اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از ﺑﺮرﺳﯽ ﺑﯿﺎن ژن ﻧﺸﺎن داد ﮐﻪ ﺗﻨﺶ ﺳﺮﻣﺎﯾﯽ ﺑﺮ ﻣﯿﺰان ﺑﯿﺎن ژنﻫﺎ اﺛﺮ ﻣﻌﻨﯽداري دارد و ﻣﯿﺰان ﺑﯿﺎن ژن TPS5 در ﺑﺎزهﻫﺎي ﻣﺘﻔﺎوت زﻣﺎﻧﯽ ﺗﻨﺶ اﻓﺰاﯾﺶ ﯾﺎﻓﺖ و در ﺑﺎزه زﻣﺎﻧﯽ 48 ﺳﺎﻋﺖ ﺑﻪ ﺑﯿﺸﺘﺮﯾﻦ ﺑﯿﺎن رﺳﯿﺪ و در ژن TPS2 ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان ﺑﯿﺎن در ﺑﺎزه زﻣﺎﻧﯽ 3 ﺳﺎﻋﺖ 3/46 ﺑﺮاﺑﺮ ﻧﺴﺒﺖ ﺑﻪ ﺷﺎﻫﺪ ﻣﺸﺎﻫﺪه ﺷﺪ.

Thyme (Thymus vulgaris L.) is xerophytes plant that can tolerat against drought and water loss stress easily. Most important secondary metabolites in Thyme are Thymol that have medicinal, antioxidant and antimicrobial properties. No research has carried out so far on the effects of cold stress on the synthesis of Thymol pathway genes and on the physiological and biochemical characteristics. This study was designed to investigate the effect of low temperature stress (4 °C) on the expression Thymol pathway genes such as TPS2 and TPS5 genes using RT-PCR in the time period of 0, 3, 12, 24 and 48 hours after treatment. To evaluate the levels of relative expression of these genes for the synthesis of Thymol (MVA, MEP) t-test was used. TPS2 gene has highest expression within the 3 hours after treatment (3.46 times) compared to the control.