پديد آورندگان :
ﻓﺮزاﻧﻪ، ﻣﺮﯾﻢ داﻧﺸﮕﺎه داﻣﻐﺎن - داﻧﺸﮑﺪه زﯾﺴﺖ ﺷﻨﺎﺳﯽ - گروه ﻋﻠﻮم ﮔﯿﺎﻫﯽ، داﻣﻐﺎن، اﯾﺮان , اﻣﯿﺮاﺣﻤﺪي، ﻋﺎﻃﻔﻪ داﻧﺸﮕﺎه داﻣﻐﺎن - داﻧﺸﮑﺪه زﯾﺴﺖ ﺷﻨﺎﺳﯽ - گروه ﻋﻠﻮم ﮔﯿﺎﻫﯽ، داﻣﻐﺎن، اﯾﺮان , ﭘﻮزش، وﺣﯿﺪ داﻧﺸﮕﺎه داﻣﻐﺎن - داﻧﺸﮑﺪه زﯾﺴﺖ ﺷﻨﺎﺳﯽ - گروه ﻋﻠﻮم ﮔﯿﺎﻫﯽ، داﻣﻐﺎن، اﯾﺮان , ﺳﻠﯿﻤﯽ، ﻓﺎﻃﻤﻪ داﻧﺸﮕﺎه داﻣﻐﺎن - داﻧﺸﮑﺪه زﯾﺴﺖ ﺷﻨﺎﺳﯽ - ﺮوه ﺳﻠﻮﻟﯽ و ﻣﻠﮑﻮﻟﯽ، داﻣﻐﺎن، اﯾﺮان
كليدواژه :
آﻧﺘﯽ اﮐﺴﯿﺪان , اﻟﺒﺮز ﺷﺮﻗﯽ , ﺑﺮازﻣﺒﻞ , ﺟﻤﻌﯿﺖﻫﺎي ﻣﺨﺘﻠﻒ .Perovskia abrotanoides Kar , ﻓﯿﺘﻮﺷﯿﻤﯽ
چكيده فارسي :
اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﺑﺮرﺳﯽ ﺗﻨﻮع ﻓﯿﺘﻮﺷﯿﻤﯿﺎﯾﯽ و ﺧﻮاص آﻧﺘﯽ اﮐﺴـﯿﺪاﻧﯽ ﺳـﻪ ﺟﻤﻌﯿـﺖ از ﮔﻮﻧـﻪ .Perovskia abrotanoides Kar در ﻣﺤﺪوده رﺷﺘﻪ ﮐﻮه اﻟﺒﺮز ﺷﺮﻗﯽ ﺷﺎﻣﻞ اﺳﺘﺎنﻫﺎي ﺳﻤﻨﺎن و ﻣﺎزﻧﺪران ﻣﯽﭘﺮدازد. ﻧﻤﻮﻧﻪ ﻫﺎي ﮔﻠﺪار ﮔﯿﺎه از ﺳﻪ روﯾﺸﮕﺎه ﻃﺒﯿﻌﯽ داﻣﻐﺎن اﯾﺴﺘﮕﺎه 1، 1504 ﻣﺘﺮ(، ﮐﺮدﻣﯿﺮ )اﯾﺴﺘﮕﺎه 2، 1672 ﻣﺘﺮ( و ﺷﺎﻫﺮود )اﯾﺴﺘﮕﺎه 3، 1285 ﻣﺘﺮ( در ﺗﯿﺮ ﻣـﺎه .1397ﺟﻤـﻊ آوري ﺷـﺪﻧﺪ. ﻣﯿﺰان ﭘﺮوﻟﯿﻦ، ﭘﺮوﺗﺌﯿﻦ، ﻗﻨﺪﻫﺎي ﻣﺤﻠﻮل، ﺗﺮﮐﯿﺒﺎت ﻓﻨﻠﯽ، ﭘﺮاﮐﺴﯿﺪ ﻫﯿﺪروژن، ﭘﺘﺎﺳﯿﻢ، ﻣﺲ، روي، آﻫﻦ، ﻧﯿﮑﻞ، ﻣﻨﯿﺰﯾﻢ، ﻣﻨﮕﻨﺰ و ﮐﻠﺴﯿﻢ در ﺳﺮﺷﺎﺧﻪﻫﺎي ﮔﻠﺪار ﮔﯿﺎه و ﻫﻤﭽﻨﯿﻦ ﻣﯿﺰان ﺷﻮري ﺧﺎك ﻫﺮ روﯾﺸﮕﺎه اﻧﺪازه ﮔﯿﺮي ﺷﺪﻧﺪ. ﻋﺼﺎرهﻫـﺎي ﻣﺘـﺎﻧﻮ ﻟﯽ، اﺗﯿـﻞ اﺳـﺘﺎﺗﯽ، دي ﮐﻠﺮوﻣﺘﺎﻧﯽ و ﻫﮕﺰاﻧﯽ ﻧﯿﺰ ﺑﻪ روش ﺧﯿﺴﺎﻧﺪن اﺳﺘﺨﺮاج و ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﻋﺼـﺎره ﻫـﺎ ﺑـ ﺎ اﺳـﺘﻔﺎده از ﻇﺮﻓﯿـﺖ ﻣﻬـﺎر رادﯾﮑﺎل ﻫﺎي DPPH ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. آزﻣﺎﯾﺶ ﺑﻪ ﺻﻮرت ﻃﺮح ﮐﺎﻣﻼً ﺗﺼﺎدﻓﯽ ﺑﺎ 3 ﺗﮑﺮار و ﻣﻘﺎﯾﺴـﻪ ﻣﯿـﺎﻧﮕﯿﻦ دادهﻫـﺎ ﺑـﺎ اﺳﺘﻔﺎده از آزﻣﻮن داﻧﮑﻦ در ﺳﻄﺢ اﺣﺘﻤﺎل 5 درﺻﺪ اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺪار ﭘـﺮوﻟﯿﻦ و ﭘﺘﺎﺳـﯿﻢ از اﯾﺴـﺘﮕﺎه 1، ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺪار ﭘﺮوﺗﺌﯿﻦ، ﺗﺮﮐﯿﺒﺎت ﻓﻨﻮﻟﯽ، ﻣﻨﯿﺰﯾﻢ و ﺷﻮري ﺧﺎك از اﯾﺴﺘﮕﺎه 3 و ﺑﺎﻻﺗﺮﯾﻦ ﻣﻘـﺪار آﻫـﻦ از اﯾﺴـﺘﮕﺎه 2 اﻧـﺪازه ﮔﯿـﺮي ﺷﺪﻧﺪ. ﻫﻤﭽﻨﯿﻦ اﯾﺴﺘﮕﺎه 3 داراي ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ در ﺗﻤﺎم ﻋﺼﺎرهﻫﺎ و ﺗﻤﺎﻣﯽ اﯾﺴﺘﮕﺎهﻫﺎ داراي ﮐﻤﺘﺮﯾﻦ ﻣﯿـﺰان ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ در ﻋﺼﺎره ﻫﮕﺰاﻧﯽ ﺑﻮدﻧﺪ. ﻋﺼﺎره ﻫﺎي دي ﮐﻠﺮوﻣﺘﺎﻧﯽ در اﯾﺴﺘﮕﺎهﻫﺎي 2 و 3 داراي ﺑﺎﻻﺗﺮﯾﻦ ﻣﯿـﺰان ﻓﻌﺎﻟﯿـﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﺑﻮدﻧﺪ در ﺣﺎﻟﯽ ﮐﻪ در اﯾﺴﺘﮕﺎه 1 ﺑﺎﻻﺗﺮﯾﻦ ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴـﯿﺪاﻧﯽ در ﻋﺼـﺎره ﻣﺘـﺎﻧﻮﻟﯽ ﯾﺎﻓـﺖ ﺷـﺪ. ﺑﻨـﺎﺑﺮاﯾﻦ ﻣﺸﺨﺺ ﺷﺪ ﮐﻪ ﺑﺎ اﻓﺰاﯾﺶ ﻣﯿﺰان ﺷﻮري ﺧﺎك ﻣﺤﺘﻮاي ﻓﻨﻠﯽ و ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﺟﻤﻌﯿﺖﻫﺎي ﮔﯿﺎه ﺑﺮازﻣﺒﻞ اﻓﺰاﯾﺶ ﻣﯽﯾﺎﺑﻨﺪ
چكيده لاتين :
This study was conducted to investigate the biochemical diversity and antioxidant properties
of three accessions of Perovskia abrotanoides Kar. in eastern Alborz mountain range including
Semnan and Mazandaran provinces. Flowering specimens were collected from three natural
habitats of Damghan (station 1, 1504 m), Kordmir (station 2, 1672 m) and Shahrud (station 3,
1285 m) in July 2018. The amount of proline, protein, soluble sugars, phenolic compounds,
hydrogen peroxide, potassium, copper, zinc, iron, nickel, magnesium, manganese and calcium
as well as soil salinity were measured. Methanolic, dichloromethane, ethyl acetate and hexane
extracts were extracted by soaking method and the antioxidant activity of the extracts was
evaluated using the DPPH radical scavenging capacity. The experiment was conducted as a
completely randomized design with 3 replications and the comparison of data means was
performed using Duncan's test at a probability level of 5%. The results showed that the highest
amount of proline and potassium was in station 1, the highest amount of protein, phenolic
compounds, soil salinity and magnesium content were obtained from station 3 and the highest
amount of iron was observed in station 2. Moreover, station 3 had the highest level of
antioxidant activity in all extracts, and all stations had the lowest level of antioxidant activity in
hexane extract. Dichloromethane extracts in stations 2 and 3 had the highest level of antioxidant
activity, while in station 1 the highest level of antioxidant activity was found in methanolic
extract. Therefore, we concluded that with increasing soil salinity, the phenolic content and
antioxidant activity of the populations of P. abrotanoides increase.
