اثر پپتيد زيست فعال مشتق از ماهي ساردين برلوله‌هاي اسپرم‌ساز متعاقب القاء تنش حرارتي در موش صحرايي نر بالغ

Effect of Sardine- drived (Sardinella sp.)Bioactive peptides on seminiferous tubules Following Heat Stress Induction in Mature Male Rat

آﺳﻴﺐ ﻧﺎﺷﻲ از ﺗﻨﺶ ﺣﺮارﺗﻲ در ﺑﻴﻀﻪﻫﺎ ﻳﻜﻲ از ﻋﻠﻞ ﻧﺎﺑﺎروري در ﻣﺮدان و اﻏﻠﺐ ﭘﺴﺘﺎﻧﺪاران ﻣﺤﺴﻮب ﻣﻲﺷﻮد. ﭘﭙﺘﻴﺪﻫﺎي زﻳﺴﺖ ﻓﻌﺎل ﻓﻌﺎﻟﻴﺖ ﺿﺪ اﻛﺴﺎﻳﺸﻲ ﻗﻮي در ﺑﺮاﺑﺮ رادﻳﻜﺎلﻫﺎي آزاد و ﮔﻮﻧﻪﻫﺎي اﻛﺴﻴﮋن ﻓﻌﺎل دارﻧﺪ. در اﻳﻦ ﻣﻄﺎﻟﻌﻪ، 42 ﺳﺮ ﻣﻮش ﺻﺤﺮاﻳﻲ ﻧﺮ ﺑﺎﻟﻎ ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﻲ ﺑﻪ6 ﮔﺮوه 7ﺗﺎﻳﻲ ﺷﺎﻣﻞ: ﮔﺮوه اول: ﺷﺎﻫﺪ )دﻣﺎي 22 درﺟﻪ ﺳﺎﻧﺘﻲﮔﺮاد(، ﮔﺮوه دوم: ﭘﭙﺘﻴﺪ زﻳﺴﺖ ﻓﻌﺎل )10 ﻣﻴﻠﻲ ﮔﺮم ﺑﺮ ﻛﻴﻠﻮﮔﺮم وزن ﺑﺪن ﺑﻪ ﺻﻮرت ﺧﻮراﻛﻲ، روزاﻧﻪ(، ﮔﺮوه ﺳﻮم: ﺗﺤﺖ ﺷﻮك ﺣﺮارﺗﻲ 43 درﺟﻪﺳﺎﻧﺘﻲﮔﺮاد، ﮔﺮوه ﻫﺎي ﭼﻬﺎرم، ﭘﻨﺠﻢ و ﺷﺸﻢ ﺗﺤﺖ ﺷﻮك ﺣﺮارﺗﻲ43 درﺟﻪ ﺳﺎﻧﺘﻲﮔﺮاد ﺑﻪ ﻫﻤﺮاه ﺗﻴﻤﺎر ﺑﺎ ﭘﭙﺘﻴﺪ زﻳﺴﺖ ﻓﻌﺎل ﺑﺎ دوز 5، 10 و 20 ﻣﻴﻠﻲﮔﺮم ﺑﺮ ﻛﻴﻠﻮﮔﺮم وزن ﺑﺪن ﺑﻪ ﺻﻮرت ﺧﻮراﻛﻲ ﺗﻘﺴﻴﻢ ﺷﺪﻧﺪ. ﺑﻌﺪ از اﺗﻤﺎم دوره ﺗﻴﻤﺎر) 45روز(، ﺑﺎﻓﺖ ﺑﻴﻀﻪ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﻲﻫﺎي ﺑﺎﻓﺖ- ﺷﻨﺎﺳﻲ و ﺑﻴﻮﺷﻴﻤﻴﺎﻳﻲ ﺟﺪاﺳﺎزي ﺷﺪ. آﺳﻴﺐ ﺷﻨﺎﺳﻲ ﺑﺎﻓﺘﻲ، ﺗﺨﺮﻳﺐ ﺑﺎﻓﺘﻲ ﮔﺴﺘﺮدهاي را ﺑﻪ دﻧﺒﺎل اﻟﻘﺎء ﺗﻨﺶ ﺣﺮارﺗﻲ در ﺑﺎﻓﺖ ﺑﻴﻀﻪ ﻧﺸﺎن داد. ﺗﻨﺶ ﺣﺮارﺗﻲ ﺳﺒﺐ اﻓﺰاﻳﺶ ﻣﻌﻨﻲدار ﻧﻴﺘﺮﻳﻚ اﻛﺴﺎﻳﺪ ﮔﺮدﻳﺪ )P<0/05(. در ﻣﻮشﻫﺎي ﺗﺤﺖ ﺗﻨﺶ ﺣﺮارﺗﻲ درﻣﺎن ﺷﺪه ﺑﺎ ﭘﭙﺘﻴﺪ زﻳﺴﺖ ﻓﻌﺎل دوز 5 و 10 ﻣﻴﻠﻲﮔﺮم ﺑﺮ ﻛﻴﻠﻮﮔﺮم ﺑﻬﺒﻮدي ﻣﺸﺎﻫﺪه ﻧﺸﺪ. ﺗﻴﻤﺎر ﺣﻴﻮاﻧﺎت ﺑﺎ دوز 20 ﻣﻴﻠﻲﮔﺮم ﺑﺮ ﻛﻴﻠﻮﮔﺮم آﺛﺎر ﻣﺜﺒﺘﻲ ﺑﺮ اﺳﭙﺮﻣﺎﺗﻮژﻧﺰ و ﺳﺎﺧﺘﺎر ﻟﻮﻟﻪﻫﺎي اﺳﭙﺮمﺳﺎز ﻧﺸﺎن داد. ﻧﺘﺎﻳﺞ ﺑﺮرﺳﻲ ﻣﻴﺰان ﻧﻴﺘﺮﻳﻚ اﻛﺴﺎﻳﺪ،ﻛﺎﻫﺶ اﻳﻦ ﻓﺎﻛﺘﻮر ﺑﻴﻮﺷﻴﻤﻲ را ﺑﻪ دﻧﺒﺎل ﺗﻴﻤﺎر ﺑﺎ ﭘﭙﺘﻴﺪ ﺑﺎ دوز 20 ﻣﻴﻠﻲﮔﺮم ﺑﺮ ﻛﻴﻠﻮﮔﺮم ﻧﺸﺎن داد )P<0/05(. ﻧﺘﺎﻳﺞ ﺣﺎﺻﻞ از اﻳﻦ ﻣﻄﺎﻟﻌﻪ آﺛﺎر آﻧﺘﻲاﻛﺴﻴﺪاﻧﺘﻲ ﭘﭙﺘﻴﺪ زﻳﺴﺖ ﻓﻌﺎل ﻣﺸﺘﻖ از ﻣﺎﻫﻲ ﺳﺎردﻳﻦ را در ﺷﺎﺧﺺﻫﺎي ﺑﺎﻓﺘﻲ و ﺑﻴﻮﺷﻴﻤﻲ ﺑﺎﻓﺖ ﺑﻴﻀﻪ ﻧﺸﺎن داد.

Damage due to thermal stress in the testicles is one of the causes of infertility in men and most of the mammals. Bioactive peptides have potent antioxidant activity against free radicals and reactive oxygen species. In this study, animals were randomly categorized into 6 groups; group 1 served as a control(22 ◦C), group 2 was a bioactive peptide(10 mg kg-1 day, oral gavage, respectively) , group 3 was a heat-stressed (43 ◦C), groups 4,5 and 6 were heat-stressed(43 ◦C) along with bioactive peptides (5, 10 and 20 mg kg-1 day, oral gavage, respectively). After treatment (45 days), the testes were separated for histological and biochemical studies. Histopathological analysis revealed severe testicular damage following heat stress induction. Heat stress increased nitric oxide significantly(P