بررسي آلودگي موش‌هاي آزمايشگاهي نژاد NIH مؤسسه رازي به باكتري استرپتوباسيلوس مونيلي‌فورميس عامل بيماري تب گازگرفتگي موش

Survey of Razi Institute NIH Laboratory mice to Streptobacillus monili-formis, the causative agent of rat bite fever

اﻃﻤﯿﻨــﺎن از ﺳــﻼﻣﺖ و ﮐﯿﻔﯿــﺖ ﺑــﺎﻻي ﻣــﻮاد ﻏــﺬاﯾﯽ در ﻃــﻮل ﻣﺮاﺣــﻞ ﺗﻮﻟﯿــﺪ، اﻧﺒــﺎرداري و ﻣﺼــﺮف از ﺟﻤﻠــﻪ دﻏﺪﻏــﻪﻫﺎي اﺻﻠﯽ ﺗﻮﻟﯿﺪﮐﻨﻨﺪﮔﺎن ﻣﯽﺑﺎﺷﺪ. ﺑـﺎ ﺗﻮﺟـﻪ ﺑـﻪ وﺟـﻮد ﻧﮕﺮاﻧـﯽ در ﺧﺼـﻮص ﺑـﯽﺧﻄـﺮ ﺑـﻮدن ﺗﺮﮐﯿﺒـﺎت ﺿـﺪ ﻣﯿﮑﺮوﺑـﯽ ﺷـﯿﻤﯿﺎﯾﯽ، اﺳــﺘﻔﺎده از ﮔﯿﺎﻫــﺎن داروﯾــﯽ و ﺧــﻮاص ﺳــﻼﻣﺘﯽ ﺑﺨــﺶ آﻧﻬــﺎ در ﺻــﻨﻌﺖ ﻏــﺬا ﻣﯽﺗﻮاﻧــﺪ ﺳــﻼﻣﺖ ﻣﺼــﺮفﮐﻨﻨﺪه را ﺗﻀــﻤﯿﻦ ﯾﮑــﯽ از ﺑﯿﻤــﺎريﻫــﺎي ﻣﻬــﻢ و ﺧﻄﺮﻧــﺎك ﺑﺎﮐﺘﺮﯾــﺎﯾﯽ در ﮐﻠﻨــﯽ ﻣــﻮشﻫــﺎي آزﻣﺎﯾﺸــﮕﺎﻫﯽ ﺗــﺐ ﮔــﺎز ﮔﺮﻓﺘﮕــﯽ ﻣــﻮش اﺳــﺖ. اﯾﻦ ﺑﯿﻤﺎري ﻗﺎﺑﻞ اﻧﺘﻘﺎل ﺑـﻪ اﻧﺴـﺎن ﻣـﯽﺑﺎﺷـﺪ. ﺑـﺮ ﻃﺒـﻖ اﺳـﺘﺎﻧﺪاردﻫﺎي ﺑـﯿﻦاﻟﻤﻠﻠـﯽ، در ﺻـﻮرت ﻣﻮاﺟـﻪ ﺷـﺪن ﺑـﺎ آن، ﺑﺎﯾﺴـﺘﯽ اﻗــﺪاﻣﺎت ﮐﻨﺘﺮﻟــﯽ، ﻣﺒــﺎرزه و اﺣﯿﺎﻧــﺎً رﯾﺸــﻪﮐﻨــﯽ و ﺣــﺬف ﮐﻠﻨــﯽ ﺻــﻮرت ﮔﯿــﺮد. ﻋﺎﻣــﻞ ﺑﯿﻤــﺎري، ﺑــﺎﮐﺘﺮي اﺳﺘﺮﭘﺘﻮﺑﺎﺳــﯿﻠﻮس ﻣﻮﻧﯿﻠﯽﻓـﻮرﻣﯿﺲ اﺳـﺖ. از ﻋﻼﺋـﻢ ﺑﯿﻤـﺎري در اﻧﺴـﺎن ﻣـﯽﺗـﻮان ﺑـﻪ ﺗـﺐ ﺷـﺪﯾﺪ، ﻟـﺮز، ﭘﻠـﯽآرﺗﺮﯾـﺖ و اﻟﺘﻬﺎﺑـﺎت ﭘﻮﺳـﺘﯽ اﺷـﺎره ﮐــﺮد. ﺗــﺎﮐﻨﻮن اﺳــﺘﺎﻧﺪاردﻫﺎي ﺑــﯿﻦاﻟﻤﻠﻠــﯽ در ﻣــﻮرد ﺗﻌﯿــﯿﻦ آﻟــﻮدﮔﯽ ﺣﯿﻮاﻧــﺎت آزﻣﺎﯾﺸــﮕﺎﻫﯽ ﺑــﻪ اﯾــﻦ ﻋﺎﻣــﻞ ﻋﻔــﻮﻧﯽ در اﯾــﺮان اﻧﺠــﺎم ﻧﮕﺮﻓﺘــﻪ اﺳــﺖ. در اﯾــﻦ ﺗﺤﻘﯿــﻖ ﻣﯿــﺰان ﺷــﯿﻮع اﯾــﻦ ﺑــﺎﮐﺘﺮي در ﮐﻠﻨــﯽ ﻣــﻮشﻫــﺎي آزﻣﺎﯾﺸــﮕﺎﻫﯽ ﻧــﮋاد NIH ﻣﺆﺳﺴــﻪ رازي در ﺳﺎل 1398 ﻣﻮرد ﺑﺮرﺳـﯽ ﻗـﺮار ﮔﺮﻓـﺖ. ﺗﻌـﺪاد 66 ﺳـﺮ ﻣـﻮش ﭘﺮورﺷـﯽ ﺑـﻪﺻـﻮرت ﺗﺼـﺎدﻓﯽ از ﯾـﮏ ﮐﻠﻨـﯽ ﭘﺮورﺷـﯽ اﻧﺘﺨــﺎب و ﺑــﺎ روش PCR از ﻧﻈــﺮ وﺟــﻮد اﯾــﻦ ﺑــﺎﮐﺘﺮي، ﻣــﻮرد ﺑﺮرﺳــﯽ ﻗــﺮار ﮔﺮﻓﺘﻨــﺪ. از اﯾــﻦ ﺗﻌــﺪاد ﻫــﯿﭻﮔﻮﻧــﻪ ﻣــﻮرد ﻣﺜﺒﺘــﯽ ﻣﺸﺎﻫﺪه ﻧﮕﺮدﯾﺪ. ﺑﺎ ﺗﻮﺟـﻪ ﺑـﻪ دﺳـﺘﻮراﻟﻌﻤﻞ ﺑـﻪﮐـﺎر ﺑـﺮده ﺷـﺪه ﻣـﯽﺗـﻮان ﻧﺘﯿﺠـﻪﮔﯿـﺮي ﻧﻤـﻮد ﮐـﻪ ﺑـﺎ اﻃﻤﯿﻨـﺎن 99/9 درﺻـﺪ، آﻟﻮدﮔﯽ ﺑﻪ ﺑـﺎﮐﺘﺮي اﺳﺘﺮﭘﺘﻮﺑﺎﺳـﯿﻠﻮس ﻣـﻮﻧﯿﻠﯽﻓـﻮرﻣﯿﺲ در ﺳـﺎﻟﻦﻫـﺎي ﭘﺮورﺷـﯽ، وﺟـﻮد ﻧﺪاﺷـﺘﻪ اﺳـﺖ. ﻧﺘﯿﺠـﻪ اﯾـﻦ ﺑﺮرﺳـﯽ، ﻧﺸــﺎندﻫﻨــﺪه اﯾــﻦ اﺳــﺖ ﮐــﻪ رﻋﺎﯾــﺖ ﻣــﻮارد ﺑﻬﺪاﺷــﺘﯽ در اﯾــﻦ ﻣﺮﮐــﺰ ﺑــﻪ ﺧــﻮﺑﯽ اﻧﺠــﺎم ﻣﯽﮔﯿــﺮد. ﮔﺮﭼــﻪ ﺑﺎﯾــﺪ ﭘــﺎﯾﺶﻫــﺎي دوره اي و ﻣﺴﺘﻤﺮ در ﻣﻮرد اﯾﻦ ﺑﺎﮐﺘﺮي و ﺳﺎﯾﺮ ﻋﻮاﻣﻞ ﻋﻔﻮﻧﯽ ﻣﻬﻢ ﺻﻮرت ﮔﯿﺮد.

One of the most important and dangerous bacterial diseases in colonies of mice is rat bite fever. This disease can be transmitted to humans. According to international standards, in the event of a confrontation, control measures, combat and possibly eradication and remove the colony should be taken. The disease caused by Streptobacillus moniliformis bacteria. Symptoms of the disease in humans include high fever, chills, polyarthritis, and skin inflammation. So far, international standards for determining the infection of laboratory animals with this infectious agent have not been performed in Iran. In this study, the prevalence of this bacterium, in the colony of Razi Institute NIH Laboratory mice was studied in 1398. Number of 66 mice from a breeding colony were randomly selected and were examined by PCR method for presence of this bacterium. No positive samples were observed. According to the instructions used, it can be concluded that with 99.9% confidence, there was no infection with Streptobacillus monilioformis in breeding facilities. The result of this study shows that the observance of hygiene items in this center is done well. However, periodic and continuous monitoring of this bacterium and other important infectious agents should be performed.