مقايسۀ نيمرخ ژنتيكي چندشكلي تك نوكلئوتيدي rs4646994 ژن ACE در مردان نخبۀ وزنه بردار با غيرورزشكاران

Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes

: ژن ACE آنزيم مبدل آنژيوتانسين (ACE) را كدگذاري مي كند كه با وجود آلل I يا نبودِ آلل D يك قطعه 287 جفت بازي مشخص مي شود. آلل D با فعاليت ACE بالاتر و افزايش سطح آنژيوتانسين II و عملكردهاي ورزشي مبتني بر قدرت همراه است؛ بنابراين، هدف مطالعۀ حاضر مقايسۀ نيمرخ ژنتيكي چندشكلي تك نوكلئوتيدي rs4646994 ژن ACE در مردان نخبۀ وزنه بردار با غيرورزشكاران بود. مواد و روش ها: 30 مرد نخبۀ وزنه بردار (قهرمانان ليگ، كشور، تيم ملي، قهرمانان آسيا، جهان و المپيك) مقيم استان اصفهان، به همراه 43 داوطلب غيرورزشكار سالم كه از لحاظ سن، قد و وزن مشابه گروه نخبه بودند، آزمودني هاي بررسي را تشكيل دادند. با تكميل فرم رضايت‌نامه، نمونه هاي بزاق از شركت كنندگان جمع آوري شد و پس از مراحل استخراج DNA، تعيين ژنوتيپ هاي ACE با استفاده از تكنيك هاي PCR_RLFP و الكتروفورز صورت گرفت. تجزيه‌وتحليل داده ها با استفاده از روش هاي آماري، t مستقل، كاي مربع و رگرسيون لجستيك از طريق نرم افزار SPSS vol.20 انجام گرديد. يافته‌ها: نتايج نشان داد شيوع آلل D به‌طور معني داري در وزنه برداران نخبه و غيرورزشكاران، بالاتر از آلل I در گروه اي خود بود (0.05

The ACE gene encodes the angiotensin-converting enzyme (ACE), which is characterized by the presence of allele I or the absence of allele D of a 287-bp fragment. The D allele is associated with higher ACE activity and increased angiotensin II levels and performance in strength-oriented exercise tasks. This study aimed to compare the genetic profile of rs4646994 single nucleotide polymorphism of the ACE gene in the elite male weightlifters and non-athletes. Material & Methods: In total, 30 elite male weightlifters (league, country, national team, Asian, world, and Olympic champions) living in Isfahan province along with 43 healthy non-athlete volunteers who were matched in age, height, and weight to the elite group formed the subjects of the study. After completing the consent form, saliva samples were collected from the participants, and after DNA extraction, ACE genotypes were determined using PCR-RLFP and electrophoresis techniques. Data analysis was performed using independent t-test, chi-square, and logistic regression in SPSS software (version 20). (Ethic code: 7993) Findings: The results showed that the prevalence of D allele in elite weightlifters and non-athletes was significantly higher than allele I in their groups (P<0.05). Moreover, in the genotyping distribution, D/I was most common both in the study population (7.50%), as well as weightlifters (7.56%) and non-athletes (5.46%). However, there was no significant difference in the elite weightlifters and non-athletes regarding the distribution of D/I ACE genotyping (χ2=0/31). Discussion & Conclusion: The study showed a positive relationship between Iranian elite weightlifters and the ACE D allele, which probably distinguishes it as one of the effective genetic factors in creating a strength phenotype of Iranian weightlifters.