كليدواژه :
رنگدانه فتوسنتزي , فعاليت آنتي اكسيداني , محتواي نسبي رطوبت , وزن خشك
چكيده فارسي :
كمبود آب يكي از مهمترين تنش هاي غير زنده است كه بر عملكرد گياهان باغي اثر نامطلوب دارد. اين در حالي است كه استفاده از تكنولوژي هاي نوين مثل نانو ذرات مي‌تواند بر بهبود عملكرد گياهان موثر باشد. به اين منظور آزمايشي به صورت فاكتوريل بر پايه طرح كاملا تصادفي با چهار تكرار روي گياه ترخون (Artemisia dracunculus) در دانشگاه فردوسي مشهد طراحي و اجرا شد. تيمارهاي آزمايشي شامل سه سطح تنش خشكي (90، 70 و 50% ظرفيت زراعي) و سه سطح نانو دي‌اكسيد‌تيتانيوم (0، 10 و 20 پي پي ام) بود. طبق نتايج بيشترين وزن خشك بخش هوايي و ريشه ترخون در تيمار 90% ظرفيت زراعي آبياري و محلول پاشي با 10 و 20 پي پي ام نانو دي‌اكسيد‌تيتانيوم و كمترين درصد فعاليت آنتي‌اكسيدانتي (49%) و درصد كربوهيدارت كل محلول (14%) در در تيمار 90% ظرفيت زراعي و در شرايط عدم محلول پاشي با نانو دي‌اكسيد‌تيتانيوم برگ بود. همچنين با افزايش غلظت دي‌اكسيد‌تيتانيوم از 0 به 10 پي پي ام قطر ريشه، ارتفاع و قطر ساقه در 90% ظرفيت زراعي به ترتيب 41.8، 39.5، و 42.2% افزايش و در 50% ظرفيت زراعي 16.4، 8.8 و 16.5 % افزايش داشت. به طور كلي اگرچه اعمال تنش خشكي منجر به كاهش صفات مورفولوژيك و رنگدانه‌هاي فتوسنتزي در ترخون شد محلول پاشي با نانو دي‌اكسيد‌تيتانيوم در دو غلظت 10 و 20 پي پي ام در بهبود اين صفات موثر بود.
چكيده لاتين :
Introduction
Iran is one of the arid and semi-arid regions of the world with an average rainfall of 240 mm per year. Water scarcity is one of the most important abiotic stresses that adversely affects the yield of garden plants. However, the use of new technologies such as nanoparticles can be effective in improving plant performance. Metal nanoparticles such as nano-titanium, zinc, iron, aluminum and silver can be effective in increasing the supply of elements in the roots and shoots of plants. Recently, the use of titanium nanoparticles has been highly regarded by plant physiologists due to its outstanding properties.
Materials and methods
For this purpose, a factorial experiment based on a completely randomized design with four replications on tarragon (Artemisia dracunculus) was designed and conducted at Ferdowsi University of Mashhad. Experimental treatments included three levels of drought stress (90, 70 and 50% of field capacity) and three levels of nano titanium dioxide (0, 10 and 20 ppm). The method of applying titanium dioxide nano treatments was foliar spraying. In this way, the above treatments were sprayed on tarragon leaves in four stages every seven days (two stages before stress and two stages after drought stress). At the end of the experiment, the following traits were measured. Plant height, number of lateral stems and number of leaves per plant were recorded. Dry weight of shoot, root and total dry weight after drying in an oven at 70 ° C for 48 hours were measured with a digital scale with an accuracy of 0.001. Stem diameter, root length and root diameter were calculated with a digital caliper and leaf area with a leaf area meter. Relative water content, electrolyte leakage, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids, total carbohydrates and percentage of antioxidant activity were measured
Results and discussion
According to the results, the highest shoot and root dry weight of tarragon were in 90% of irrigation and foliar application with 10 and 20 ppm titanium dioxide and also the lowest percentage of antioxidant activity (49%) and the percentage of total carbohydrate solution (14%) was seen in 90% FC and in the absence of foliar application with nano titanium dioxide. Root diameter, height and stem diameter increased by 41.8%, 39.5%, and 42.2% in 90% FC and 10 ppm nano titanium dioxide compare to 0 ppm nano titanium dioxide at the same drought levels (90% FC). By increasing the concentration of nanoparticles from 0 to 20 ppm these traits increased by 16.4, 8.8 and 16.5% respectively, at 50% FC. As the results show, the dry weight of the plant decreased under drought stress. In fact, plant dry weight is affected by plant growth and photosynthesis process and decreases with decreasing photosynthesis under stress conditions. On the other hand, the condition of plants in drought stress conditions is improved by the use of nano titanium and the dry weight of the shoot is increased. Studies have also shown that drought stress significantly reduces the content of chlorophyll a, b, total chlorophyll and carotenoids. On the other hand, an increase in chlorophyll content under stress conditions has been reported due to the use of nano-titanium
Conclusions
In general, although drought stress reduced morphological traits and photosynthetic pigments in tarragon, foliar application of titanium dioxide at concentrations of 10 and 20 ppm was effective in improving these traits. It has been reported that titanium nanoparticles may increase photosynthesis and efficiency by increasing light reception and increase the plant's carbohydrate production potential. Studies by Soltani et al. Have shown that titanium nano dioxide has an effect on the activity of antioxidant enzymes such as catalase, peroxidase and ascorbic peroxidase.
Acknowledgements
The special unit of horticultural services and green space engineering of Ferdowsi University of Mashhad is appreciated to cover the costs of this research.
