ﺗﺄﺛﯿﺮ ال-ﮐﺎرﻧﯿﺘﯿﻦ ﺑﺮ ﺷﺎﺧﺺﻫﺎي اﺳﭙﺮﻣﯽ و ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢﻫﺎي آﻧﺘﯽاﮐﺴﯿﺪان در ﺳﻤﯿﺖ ﻧﺎﺷﯽ از ﺑﻮﺳﻮﻟﻔﺎن در ﻣﺪل ﺣﯿﻮاﻧﯽ ﻣﻮش ﺻﺤﺮاﯾﯽ

Effects of L-carnitien on spermatic parameters and antioxidant enzyme activity in toxicity induced by busulfan in a rat Model

ﺑﻮﺳﻮﻟﻔﺎن ﺑﻪ ﻋﻨﻮان ﯾﮏ داروي ﺷﯿﻤﯽ درﻣﺎﻧﯽ، ﺑﻪ ﺻﻮرت ﮔﺴﺘﺮده ﺑﺮاي درﻣﺎن ﺳﺮﻃﺎﻧﻬﺎﯾﯽ ﻣﺎﻧﻨﺪ ﻟﻨﻔﻮم ، ﻟﻮﺳﻤﯽ و در ﻣﻮارد ﭘﯿﻮﻧﺪ اﺳﺘﺨﻮان اﺳﺘﻔﺎده ﻣﯽ ﺷﻮد، ﺑﻨﺎﺑﺮاﯾﻦ ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ارزﯾﺎﺑﯽ اﺛﺮات آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ال-ﮐﺎرﻧﯿﺘﯿﻦ ﺑﺮ ﺷﺎﺧﺺﻫﺎي اﺳﭙﺮم و ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢﻫﺎي آﻧﺘﯽ اﮐﺴﯿﺪان )ﺳﻮﭘﺮاﮐﺴﯿﺪ دﯾﺴﻤﻮﺗﺎز و ﮐﺎﺗﺎﻻز( و ﻏﻠﻈﺖ ﻣﺎﻟﻮن دي اﻟﺪﺋﯿﺪدر ﺑﺎﻓﺖ ﺑﯿﻀﻪ در ﻣﺪل ﺣﯿﻮاﻧﯽ رت ﺑﻮد. 24ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر ﺑﺎ وزن ﺗﻘﺮﯾﺒﯽ 220ﺗﺎ 250ﮔﺮم ﺑﻪ ﻃﻮر ﺗﺼﺎدﻓﯽ ﺑﻪ 4ﮔﺮوه ﻣﺴﺎوي ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ : ﮔﺮوه ﺑﻮﺳﻮﻟﻔﺎن، ﮔﺮوه ال-ﮐﺎرﻧﯿﺘﯿﻦ+ ﺑﻮﺳﻮﻟﻔﺎن، ﮔﺮوه ﺷﻢ و ﮔﺮوه ﮐﻨﺘﺮل. ﮔﺮوه ﺑﻮﺳﻮﻟﻔﺎن و ال-ﮐﺎرﻧﯿﺘﯿﻦ+ﺑﻮﺳﻮﻟﻔﺎن 25ﻣﯿﻠﯽﮔﺮم/ﮐﯿﻠﻮﮔﺮم ﺑﻮﺳﻮﻟﻔﺎن داﺧﻞ ﺻﻔﺎﻗﯽ در زﻣﺎن ﺷﺮوع و 10ﻣﯿﻠﯽﮔﺮم/ﮐﯿﻠﻮﮔﺮم 14روز ﺑﻌﺪ از ﺗﺰرﯾﻖ اول درﯾﺎﻓﺖ ﮐﺮدﻧﺪ. ﮔﺮوه ال-ﮐﺎرﻧﯿﺘﯿﻦ+ ﺑﻮﺳﻮﻟﻔﺎن 100ﻣﯿﻠﯽﮔﺮم/ﮐﯿﻠﻮﮔﺮم/روزاﻧﻪ درﯾﺎﻓﺖ ﮐﺮدﻧﺪ.ﮔﺮوه ﺷﻢ دي ﻣﺘﯿﻞ ﺳﻮﻟﻔﻮاﮐﺴﯿﺪ )DMSO( 0/2ﻣﯿﻠﯽﻟﯿﺘﺮ ﺑﻪ ازاي ﻫﺮ رت ﺑﻪ ﻋﻨﻮان ﺣﻼل ﺑﻮﺳﻮﻟﻔﺎن و ﮔﺮوه ﮐﻨﺘﺮل0/2 ﻣﯿﻠﯽﻟﯿﺘﺮ ﻧﺮﻣﺎل ﺳﺎﻟﯿﻦ داﺧﻞ ﺻﻔﺎﻗﯽ روزاﻧﻪ درﯾﺎﻓﺖ ﮐﺮدﻧﺪ. ﺷﺎﺧﺺﻫﺎي اﺳﭙﺮﻣﯽ ﻣﺎﻧﻨﺪ ﻣﯿﺎﻧﮕﯿﻦ ﺗﻌﺪاد اﺳﭙﺮم در دم اﭘﯿﺪﯾﺪﯾﻢ، اﺳﭙﺮم زﻧﺪه و اﺳﭙﺮم ﻫﺎي ﻣﺘﺤﺮك در ﻫﺮ ﮔﺮوه ﺷﻤﺎرش و ﻣﻘﺎﯾﺴﻪ ﺷﺪ. ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢﻫﺎي ﺳﻮﭘﺮاﮐﺴﯿﺪدﯾﺴﻤﻮﺗﺎز، ﮐﺎﺗﺎﻻز و ﻣﯿﺰان ﻣﺎﻟﻮن دي اﻟﺪﺋﯿﺪ و ﻣﯿﺰان ﭘﺮوﺗﺌﯿﻦ ﺗﺎم و ﻧﺴﺒﺖ ﻫﺮ آﻧﺰﯾﻢ ﺑﻪ ﭘﺮوﺗﺌﯿﻦ ﺗﺎم در ﺑﯿﻀﻪ رتﻫﺎ اﻧﺪازه ﮔﯿﺮي و ﻣﻘﺎﯾﺴﻪ ﺷﺪ. ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از ﺷﺎﺧﺺﻫﺎي اﺳﭙﺮم ﻧﺸﺎن داد ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺎﻧﮕﯿﻦ ﺗﻌﺪاد اﺳﭙﺮم و درﺻﺪ اﺳﭙﺮم ﻫﺎي ﻣﺘﺤﺮك و اﺳﭙﺮم زﻧﺪه در ﮔﺮوه ﮐﻨﺘﺮل و ﺳﭙﺲ ﮔﺮوه ﻫﺎي ﺷﻢ، ال-ﮐﺎرﻧﯿﺘﯿﻦ+ ﺑﻮﺳﻮﻟﻔﺎن و ﺑﻮﺳﻮﻟﻔﺎن ﺑﻮد. ﻣﯿﺎﻧﮕﯿﻦ ﻫﺮ ﺳﻪ ﺷﺎﺧﺺ اﺳﭙﺮم ﺑﻪ ﻃﻮر ﻣﻌﻨﯽداري در ﮔﺮوه ﺑﻮﺳﻮﻟﻔﺎن ﻧﺴﺒﺖ ﺑﻪ ﻫﻤﻪ ﮔﺮوهﻫﺎ ﺗﻔﺎوت داﺷﺖ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان ﻣﺎﻟﻮن دي اﻟﺪﯾﺌﺪ و ﮐﻤﺘﺮﯾﻦ ﻓﻌﺎﻟﯿﺖ ﺳﻮﭘﺮاﮐﺴﯿﺪدﯾﺴﻤﻮﺗﺎز و ﮐﺎﺗﺎﻻز در ﮔﺮوه ﺑﻮﺳﻮﻟﻔﺎن، ﺳﭙﺲ ال-ﮐﺎرﻧﯿﺘﯿﻦ+ ﺑﻮﺳﻮﻟﻔﺎن ، ﺷﻢ و ﮔﺮوه ﮐﻨﺘﺮل ﺑﻮد. درﺣﺎﻟﯽ ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻤﯽ ﮐﺎﺗﺎﻻز و ﺳﻮﭘﺮاﮐﺴﯿﺪ دﯾﺴﻤﻮﺗﺎز و ﮐﻤﺘﺮﯾﻦ ﻣﯿﺰان ﻣﺎﻟﻮن دي آﻟﺪﺋﯿﺪ ﻣﺮﺑﻮط ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل و ﺳﭙﺲ ﺷﻢ ﺑﻮد ، ﺑﻨﺎﺑﺮاﯾﻦ ال-ﮐﺎرﻧﯿﺘﯿﻦ ﻧﻘﺎﯾﺺ اﯾﺠﺎد ﺷﺪه در ﺷﺎﺧﺺﻫﺎي اﺳﭙﺮﻣﯽ ﻧﺎﺷﯽ از اﻟﻘ ﺎي اﺳﺘﺮس اﮐﺴﯿﺪاﺗﯿﻮ ﺑﺎ ﺑﻮﺳﻮﻟﻔﺎن را ﺑﻬﺒﻮد ﻣﯽ ﺑﺨﺸﺪ.

Busulfan, a chemotherapy agent, is widely administrated for treatment of lymphoma and leukemia as well as bone transplantation. Therefore, the aim of this study was to evaluate the anti-oxidant effects of L-carnitine on sperm parameters and anti-oxidant enzymes activity (SOD and catalase), and MDA concentration in the busulflan- impaired testis tissue of rat animal model. A total of 24 Wistar rats, 220-250g, were randomly divided into 4 equal groups including the busulfan, busulfan+L-carnintine, Sham, and control groups. Busulfan was diluted in DMSO and intra-peritoneally (IP) administrated in two doses 25 and 10 mg/kg with 14-day interval in the busulfan and busulfan+L-carnitine groups. Administration of L-carnitine was daily started 1day after busulfan injection for 28day. The sham and control groups daily received DMSO, and PBS, respectively. After 28 days, the animals were euthanized and the semen was collected from the epididymis and the testicular tissues were removed to assess sperm parameters and biochemical analysis including SOD, catalase and MDA. The highest sperm parameters were belonged to control followed by sham, busulfan+L-carnitine and busulfan groups. The highest level of MDA and the lowest SOD and catalase activity was belonged to busulfan followed by busulfan+L-carnitine, sham and control groups. In conclusion, administration of L-carnitine can attenuate busulfan-induced stress oxidative injuries of sperm parameters in rats.