بررسي بيان ژنهاي HSPA2 و HSPB1 و هستيشناسي ژن در كبد مرغان بومي خراسان تحت تنش گرمايي حاد

A study on the expression of HASPA2 and HSPB1 genes in the liver of Khorasan native chickens under acute heat stress

دماي بالا يكي از فاكتورهاي اصلي محيطي مؤثر بر كاهش سود اقتصادي واحدهاي پرورش طيور است، زيرا رشد و توليد پرنده را مختل ميكند. پروتئينهاي شوك گرمايي (HSPs) نقش كليدي در مكانيسم دفاعي سلولي عليه گرماي محيط دارند. هدف از انجام مطالعه حاضر، بررسي ميزان بيان ژنهاي HSPA2 و HSPB1 در كبد مرغان بومي خراسان تحت تنش گرمايي حاد بود. براي اين هدف، تعداد 16 قطعه جوجه در سن 42 روزگي به دو گروه شاهد (دماي C 25 و رطوبت %50) و تيماري (دماي 40 و C 42 و رطوبت %50 به مدت 24 ساعت) تقسيم شده و پس از كشتار از كبد آنها نمونهبرداري به عمل آمد. سپس ميزان بيان ژنهاي HSPA2 و HSPB1 با روش RT-qPCR مورد ارزيابي قرار گرفت. بيان ژن HSPA2 نسبت به گروه شاهد افزايش معنيداري داشت (05/0

Heat stress is one of the main environmental factors causing economic losses to the poultry industry, as it reduces growth and production performance of chickens. The heat shock proteins (HSPs) involved in the key cellular defense mechanisms during exposure in hot environmental conditions. Elevated level of expression of HSPs helps to overcome the stress caused by high temperatures. The aim of this study was to evaluate the expression level of HSPA2 and HSPB1 transcripts in the liver of Khorasan native chickens under acute heat stress. Sixteen 42days old chickens were divided into two groups of control (25 C and 50% humidity) and heat treated (40 and 42 C, 50% humidity for 24h), and then the liver was sampled after killing. The transcription level of HSPA2 and HSPB1 was evaluated using RT-qPCR method. The HSPA2 expression was significantly induced (P0.05). However, the expression of HSPB1 did not show any change. The analysis of protein-protein interaction revealed that HSPA2 and HSPB1 had no interaction, however, four pathways in gene ontology including Protein processing in endoplasmic reticulum, Progesterone-mediated oocyte maturation, MAPK signaling pathway and VEGF signaling pathway had the highest significancy. The results indicated that Khorasan native chickens have suitable genetic reaction to acute heat stress and HSPA2 has the ability to express under high ambient temperature to protect the structure of cellular proteins.