اثرات سطوح سولفات روي و سلنيوم در جيره‌هاي غذايي حاوي روغن اكسيده بر عملكرد رشد، كيفيت گوشت و سيستم دفاع پاداكسندگي جوجه‌هاي گوشتي

The Effects of supplementation of zinc sulfate and selenium in diets containing oxidized oil on growth performance, meat quality, and oxidative indices of broilers chickens.

به منظور ارزيابي اثرات سطوح روي و سلنيوم معدني در جيره‌هاي غذايي حاوي روغن اكسيده بر عملكرد، ميزان فعاليت آنزيم‌هاي پاد‌اكسندگي و كيفيت گوشت جوجه‌هاي گوشتي، آزمايشي با استفاده از 480 قطعه جوجه گوشتي يك روزه سويه راس 308، در قالب آزمايش فاكتوريل 2*3*2 . به منظور بررسي ميزان پايداري گوشت در سن 42 روزگي، ميزان مالون دي آلدئيد (MDA) از نمونه‌هاي گوشت ران ذخيره شده بعد از گذشت يك ماه از كشتار مورد بررسي قرار گرفتند. ميزان فعاليت سرمي آنزيم‌هاي سوپر اكسيد ديسموتاز (SOD) و گلوتاتيون پراكسيداز (GPX) با خون‌گيري از طريق وريد بال جوجه‌ها قبل از كشتار انجام شد. نتايج نشان داد كه ميانگين مصرف خوراك روزانه و ضريب تبديل جوجه‌ها، تحت تاثير جيره‌هاي آزمايشي قرار نگرفت. اما ميانگين افزايش وزن روزانه، تحت تاثير ميزان مصرف روي، اختلاف معني‌داري نشان داد(05/0P<). سطح 100 ميلي‌گرم، روي در مقايسه با شاهد تفاوت معني‌داري بر ميزان فعاليت آنزيم SOD نشان داد (05/0P

This experiment was conducted to evaluate the effects of supplementation of zinc and inorganic selenium in diets containing oxidized oil on performance, antioxidant enzymes activity, and meat quality of broiler chickens. A total of 480 day-old Ross 308 broiler chickens were randomly attributed into 12 treatments with four replicates each with 10 chickens in 2×3×2 factorial arrangement, includes: Type of oil (fresh and 2% sunflower oxidized), zinc sulfate (0, 40 and, 100 mg/kg of diet), and selenium (0 and 0.3 mg/kg of diet). Feed intake (FI), body weight gains (BWG) and, feed conversion ratio (FCR) was measured throughout the entire period of experiment. At the age of 42, one bird per each replicate was randomly selected, bled and then killed. The thigh was dissected and muscles were removed to assess the malondialdehyde (MDA) content. The results showed that the birds’ average daily FI and FCR were not significantly influenced by dietary treatments. Inversely, the average daily BWG showed a significant difference among treatments over the experimental period (P <0.05). The birds received dietary Zinc at the level of 100 mg showed a higher activity of SOD enzyme (P <0.05). The lowest amounts of GPX was observed in birds fed diets containing oxidized oils, no dietary zinc and selenium at the level of 3.0 mg/kg. The highest and lowest levels of thigh meat MDA levels were noted in birds received diets containing oxidized oil with no dietary zinc and those had given fresh oil diets with supplementary zinc at 100 mg kg zinc and supplementary Se at 0.3 mg/kg, respectively. The results of this study revealed no synergistic effects between zinc and selenium on oxidative enzymes and MDA levels. It is recommended to supplement 100 mg / kg of zinc and 0.3 mg / kg of selenium to reduce the peroxidation effects of dietary oxidized oil.