مطالعه هيستوپاتولوژيكي ضايعات ريوي و شناسايي مولكولي پاستورلوز و مايكوپلاسموز در كشتارگاه فريمان

Histopathological study of pulmonary lesions and molecular detection of pasteurellosis and mycoplasmosis in Fariman slaughter house

ﭘﺎﺳــﺘﻮرﻟﻮز و ﻣﺎﯾﮑﻮﭘﻼﺳــﻤﻮز ﻣﻬﻢﺗــﺮﯾﻦ ﺑﯿﻤﺎريﻫــﺎي ﺗﻨﻔﺴــﯽ ﺑﺎﮐﺘﺮﯾــﺎﯾﯽ ﻫﺴــﺘﻨﺪ ﮐــﻪ از ﻟﺤــﺎظ اﻗﺘﺼــﺎدي ﺳــﺒﺐ ﮐــﺎﻫﺶ ﺗﻮﻟﯿﺪ، ﮐـﺎﻫﺶ وزن، اﻓـﺰاﯾﺶ ﻣﯿـﺰان ﻣـﺮگ و ﻣﯿـﺮ در ﮔﻠـﻪﻫﺎي ﮔﻮﺳـﻔﻨﺪان ﺷـﯿﺮي و ﮔﻮﺷـﺘﯽ ﻣﯽﺷـﻮﻧﺪ. اﯾـﻦ ﻣﻄﺎﻟﻌـﻪ ﺟﻬـﺖ ﺗﻌﯿــﯿﻦ ﺧﺼﻮﺻــﯿﺎت ﻫﯿﺴــﺘﻮﭘﺎﺗﻮﻟﻮژﯾﮑﯽ و ﻣﻮﻟﮑــﻮﻟﯽ ﺿــﺎﯾﻌﺎت رﯾــﻮي اﯾﺠــﺎد ﺷــﺪه ﺗﻮﺳــﻂ ﭘﺎﺳــﺘﻮرﻟﻮز و ﻣﺎﯾﮑﻮﭘﻼﺳــﻤﻮز در ﮔﻮﺳــﻔﻨﺪان ﮐﺸــﺘﺎر ﺷــﺪه در ﻓﺮﯾﻤــﺎن اﻧﺠــﺎم ﺷــﺪ. 4960 رﯾــﻪ از ﮔﻮﺳــﻔﻨﺪان ﮐﺸــﺘﺎر ﺷــﺪه در ﮐﺸــﺘﺎرﮔﺎه ﻓﺮﯾﻤــﺎن در ﺳــﺎلﻫﺎي 1398-1399 ﺟﻤــﻊآوري ﺷــﺪ. 90 رﯾــﻪ از 4960 )1/81 درﺻــﺪ( ﮐــﻪ از ﻟﺤــﺎظ ﻣﺎﮐﺮوﺳــﮑﻮﭘﯽ ﻣﺸــﮑﻮك ﺑــﻪ ﭘﻨﻮﻣﻮﻧﯽ ﺑﻮدﻧـﺪ ﺑﺮرﺳـﯽ ﻣﯿﮑﺮوﺳـﮑﻮﭘﯽ ﺷـﺪﻧﺪ. واﮐـﻨﺶ زﻧﺠﯿـﺮه ﭘﻠﯿﻤـﺮاز ﺟﻬـﺖ ﺷﻨﺎﺳـﺎﯾﯽ ﻣﻮﻟﮑـﻮﻟﯽ ﭘﺎﺳـﺘﻮرﻻ ﻣـﻮﻟﺘﯽﺳـﯿﺪا و ﻣﺎﯾﮑﻮﭘﻼﺳــﻤﺎ اويﻧﻮﻣﻮﻧﯿــﻪ اﻧﺠــﺎم ﺷــﺪ. ﻫﻤﭽﻨــﯿﻦ، آﻧــﺎﻟﯿﺰ آﻣــﺎري ﻣﺮﺑــﻊ ﮐــﺎي اﻧﺠــﺎم ﺷــﺪ و دادهﻫــﺎ ﺗﻮﺳــﻂ ﻧﺮماﻓــﺰار SPSS ﻧﺴــﺨﻪ 20 آﻧــﺎﻟﯿﺰ ﺷــﺪﻧﺪ. آﻧــﺎﻟﯿﺰ ﻣﻮﻟﮑــﻮﻟﯽ ﻧﺸــﺎندﻫﻨﺪه 16 ﻧﻤﻮﻧــﻪ ﻣﺜﺒــﺖ ﭘﺎﺳــﺘﻮرﻻ ﻣــﻮﻟﺘﯽﺳــﯿﺪا )0/032 درﺻــﺪ( ﺑــﻮد اﻣــﺎ ﻣﺎﯾﮑﻮﭘﻼﺳـــﻤﺎ اويﻧﻮﻣﻮﻧﯿـــﻪ در ﺗﻤـــﺎﻣﯽ ﻧﻤﻮﻧـــﻪﻫﺎ ﻣﻨﻔـــﯽ ﺑـــﻮد. در ارزﯾـــﺎﺑﯽ ﻫﯿﺴـــﺘﻮﭘﺎﺗﻮﻟﻮژﯾﮑﯽ، ﻣﺘـــﺪاولﺗﺮﯾﻦ ﺿـــﺎﯾﻌﻪ، ﺑﺮوﻧﮑﻮﭘﻨﻮﻣــﻮﻧﯽ ﭼﺮﮐــﯽ ﺑــﻮد )1/16 درﺻــﺪ(. ﺑــﻪﻋﻼوه، ارﺗﺒــﺎط آﻣــﺎري ﻣﻌﻨــﯽداري ﺑــﯿﻦ ﺳــﻦ، ﺟــﻨﺲ و ﻓﺼــﻞ ﺑــﺎ ﭘﺎﺳــﺘﻮرﻻ ﻣــﻮﻟﺘﯽﺳــﯿﺪا وﺟــﻮد ﻧﺪاﺷــﺖ )0/05 P> (. اﯾــﻦ ﯾﺎﻓﺘــﻪﻫﺎ آﺷــﮑﺎر ﮐــﺮد ﮐــﻪ ﭘﺎﺳــﺘﻮرﻻ ﻣــﻮﻟﺘﯽﺳــﯿﺪا ﯾﮑــﯽ از ﻋﻮاﻣــﻞ ﻣﺘــﺪاول ﻣﺴــﺒﺐ ﭘﻨﻮﻣــﻮﻧﯽ ﺑﺎﮐﺘﺮﯾــﺎﯾﯽ در ﮔﻮﺳــﻔﻨﺪان ﮐﺸــﺘﺎر ﺷــﺪه در ﻓﺮﯾﻤــﺎن اﺳــﺖ و ﻣﺸﺨﺼــﺎت ﻫﯿﺴــﺘﻮﭘﺎﺗﻮﻟﻮژﯾﮑﯽ آن ﻗﺎﺑــﻞ ﺗﻮﺟــﻪ ﻣﯽﺑﺎﺷﺪ.

The most economically significant bacterial pneumonias are pasteurellosis and mycoplasmosis as causative agents of reduction in production, weight loss, increasing mortality rates in dairy and fattening sheep herds. This study was conducted to determine the histopathological and molecular properties of lung lesions caused by pasteurellosis and mycoplasmosis in slaughtered sheep at Fariman. A total of 4960 lungs were collected from sheep slaughtered at Fariman abattoir in 2019-2020. 90 of 4960 (1.81%) lungs which were macroscopically suspected to be suffering from pneumonia were examined microscopically. The polymerase chain reaction test was carried out for molecular detection of Pasteurella multocida and Mycoplasma ovipneumonia. Furthermore, statistical analysis was performed using the Chi-square test and the data were analyzed by SPSS software version 20. The molecular analysis showed that 16 specimens (0.032%) were positive for P. multocida, but M. ovipneumonia was negative in all specimens. In the histopathological investigation, the most common lesion was suppurative bronchopneumonia (1.16%). In addition, there was no significant association between age, sex and season with P. multocida (P˃0.05). These findings revealed that P. multocida is one of the common cause of bacterial pneumonia in slaughtered sheep at Fariman and its histopathological characteristics are notable.