بررسي مقايسه اثر اسانس اوژنول با مترونيدازول بر تك‌ياخته تريكوموناس‌گالينه در شرايط آزمايشگاه

Evaluation of the effect of eugenol essential oil on Trichomonas galine protozoan in vitro

ﺗﺮﯾﮑﻮﻣﻮﻧﺎسﮔﺎﻟﯿﻨﻪ ﯾﮏ اﻧﮕـﻞ ﺗـﺎژكدار ﻣﺨﺼـﻮص ﺣﯿﻮاﻧـﺎت ﺧـﻮنﮔـﺮم اﺳـﺖ ﮐـﻪ ﻣﻨﺤﺼـﺮاً در ﭘﺮﻧـﺪﮔﺎن ﯾﺎﻓـﺖ ﻣـﯽﺷـﻮد و ﺑﯿﺸﺘﺮ در ﮐﺒـﻮﺗﺮ و ﻗﻤـﺮي وﺟـﻮد دارد. ﻫﻤﭽﻨـﯿﻦ ﻣﻤﮑـﻦ اﺳـﺖ ﭘﺮﻧـﺪﮔﺎن دﯾﮕـﺮ ﻧﯿـﺰ آﻟـﻮده ﺷـﻮﻧﺪ. در اﯾـﻦ ﻣﻄﺎﻟﻌـﻪ ﭘـﺲ از ﻧﻤﻮﻧﻪﺑﺮداري از ﭼﯿﻨـﻪدان ﮐﺒﻮﺗﺮﻫـﺎ، اﻧﮕﻞﻫـﺎ در ﻣﺤـﯿﻂ دﯾﺎﻣﻮﻧـﺪ ﺑـﺮاث ﮐﺸـﺖ داده ﺷـﺪﻧﺪ. ﭘـﺲ از ﮐﺸـﺖ اﻧﮕـﻞ، ﻧﻤﻮﻧـﻪﻫﺎ ﺑـﻪ ﻣــﺪت 48 ﺳــﺎﻋﺖ در دﻣــﺎي 37 درﺟــﻪ ﺳــﺎﻧﺘﯽﮔﺮاد داﺧــﻞ اﻧﮑﻮﺑــﺎﺗﻮر ﻧﮕﻬــﺪاري ﺷــﺪﻧﺪ ﺗــﺎ ﺗﻌــﺪاد ﺗﮏﯾﺎﺧﺘــﻪ ﺑــﻪ ﺣــﺪود 10000 اﻧﮕﻞ در ﻫﺮ ﻣﯿﻠﯽﻟﯿﺘﺮ ﻣﺤـﯿﻂ ﮐﺸـﺖ رﺳـﯿﺪ. ﺳـﭙﺲ در ﻫﺸـﺖ ﮔـﺮوه ﺷـﺎﻣﻞ ﯾـﮏ ﺷـﺎﻫﺪ و ﻫﻔـﺖ ﺗﯿﻤـﺎر )ﯾـﮏ ﮔـﺮوه ﻣﺘﺮوﻧﯿـــﺪازول و ﺷـــﺶ ﺗﯿﻤـــﺎر اﺳـــﺎﻧﺲ اوژﻧـــﻮل( ﻃﺒـــﻖ دوزﻫـــﺎي 25 ،12/5 ،6/25 ،3/125 ،1/56 و 50 ﻣﯿﮑﺮوﮔـــﺮم ﺑـــﺮ ﻣﯿﻠﯽﻟﯿﺘﺮ ﺑﺮاي اﯾﺠﺎد رﻗﺖﻫـﺎي ﻣـﻮرد ﻧﻈـﺮ ﺑـﻪ ﻣﺤـﯿﻂ ﮐﺸـﺖ از اوژﻧـﻮل اﺳـﺘﻔﺎده ﺷـﺪ. ﭘـﺲ از ﻗـﺮار دادن ﻣﯿﮑﺮوﭘﻠﯿـﺖﻫـﺎ در اﻧﮑﻮﺑــﺎﺗﻮر ﺑــﺎ دﻣــﺎي 37 درﺟــﻪ در ﺑــﺎزهﻫــﺎي زﻣــﺎﻧﯽ ﻣــﻮرد ﻧﻈــﺮ )1، 2، 6، 12 و 24 ﺳــﺎﻋﺖ( ﭘــﺲ از ﮐﺸــﺖ ﻣﻘــﺪار 20 ﻣﯿﮑﺮوﻟﯿﺘــﺮ از ﻫــﺮ ﭼﺎﻫــﮏ ﺑﺮداﺷــﺘﻪ و ﺑــﺎ 20 ﻻﻧــﺪا رﻧــﮓ ﺗﺮﯾﭙــﺎنﺑﻠــﻮ ﺑــﺎ ﮔﻮﺷــﻪ ﻻﻣــﻞ ﻣﺨﻠــﻮط ﮔﺮدﯾــﺪ و ﭘــﺲ از 10 دﻗﯿﻘــﻪ ﺗﻌــﺪاد اﻧﮕﻞﻫــﺎي ﻣــﺮده ﻧﺴــﺒﺖ ﺑــﻪ زﻧــﺪهﻫﺎ ﻣــﻮرد ﺷــﻤﺎرش ﻗــﺮار ﮔﺮﻓــﺖ. ﻣــﺮگ و ﻣﯿــﺮ در ﮔــﺮوه ﻣﺘﺮوﻧﯿــﺪازول در ﺗﻤــﺎﻣﯽ زﻣﺎنﻫـــﺎي ﻣﻄﺎﻟﻌـــﻪ اﺧـــﺘﻼف ﻣﻌﻨـــﯽدار را ﻧﺸـــﺎن داد )0.05 ≤p(. در ﻏﻠﻈﺖﻫـــﺎي 25 ،12/5 ،6/25 ،3/125 ،1/56 و50 ﻣﯿﮑﺮوﮔــﺮم ﺑــﺮ ﻣﯿﻠﯽﻟﯿﺘــﺮ اوژﻧــﻮل اﺧــﺘﻼف ﻣﯿــﺎن ﻣﯿــﺰان ﻣــﺮگ و ﻣﯿــﺮ در زﻣﺎنﻫــﺎي 6 و 12 ﺳــﺎﻋﺖ ﺑــﺮاي ﺗﻤــﺎﻣﯽ اﯾــﻦ ﻏﻠﻈﺖﻫــﺎ ﻣﻌﻨــﯽدار ﺑــﻮد )0.05 ≤p(. اﺧــﺘﻼف ﻣﯿــﺎن ﻣﯿــﺰان ﻣــﺮگ و ﻣﯿــﺮ در زﻣﺎنﻫــﺎي 1 و 2 ﺳــﺎﻋﺖ در ﻏﻠﻈــﺖ 1/56 ﻣﯿﮑﺮوﮔــﺮم ﺑــﺮ ﻣﯿﻠﯽﻟﯿﺘــﺮ اوژﻧــﻮل ﻣﻌﻨــﯽدار ﻧﺒــﻮد. ﻧﺘــﺎﯾﺞ ﺣﺎﺻــﻞ از ﺗﺤﻘﯿــﻖ ﮐــﺎﻣﻼً ﮔﻮﯾــﺎي اﺛــﺮ ﻣﻨﺎﺳــﺐ ﻋﺼــﺎره اوژﻧــﻮل در رﻗﺖﻫﺎي ﺑﺎﻻ )ﺑﻪ ﻏﯿﺮ ﺗﯿﻤﺎر 1/56ﻣﯿﮑﺮﮔﺮم ﺑﺮ ﻣﯿﻠﯽﻟﯿﺘﺮ( ﺑﺮ ﺗﺮﯾﮑﻮﻣﻮﻧﯿﺎزﯾﺲ اﺳﺖ.

Trichomonas galline is a flagellate parasite of warm-blooded animals found in birds that is more perceptive and lunar. Other birds may also be infected. In this study of pigeon stratigraphy, the British killed broth in a diamond environment. After culturing the parasite, the samples were kept in an incubator at 37 ° C for 48 hours until the number of protozoa reached about 1000 parasites per ml of culture medium. Then in eight groups including one control and seven treatments (one metronidazole group and six treatments of eugenol essential oil) according to doses of 1.56, 3.125, 6.25, 12.5, 25 and 5 micrograms per liter, eugenol is used to create the desired dilutions to the culture medium. . . After placing the microplate in the incubator at a temperature of 37 degrees at the desired intervals of 1, 2, 6, 12 and 24 hours after culture, 20 microliters of each well was removed and with 20 Landa trypan paint. It was mixed with the lamellar corner and after 10 minutes the number of dead Englishmen compared to the living ones was counted. Mortality in the metronidazole group showed a significant difference at all times of the study (p≤ 0.05). At 1.56, 3.125, 6.25, 12.5, 25 and 50 μg / ml eugenol, the difference between the mortality rates at 6 and 12 hours was significant for all of them (p≤ 0.05). The difference between mortality at 1 and 2 hours at 1.56 μg / L eugenol was not significant. The results of the study fully indicate the appropriate effect of eugenol extract in high dilutions (untreated (1.56 μg / ml)) on trichomoniasis.