بررسي رشد ديناميكي آماستيگوت هاي ليشمانياماژور و ليشماتروپيكا در درون ماكروفاژهاي استان

Dynamic growth of amastigotes of Leishmania major and Leishmania tropica in human macrophages

This study has been carried out on mononuclear cells of peripheral blood to identify that L.major and L.tropica parasites have life cycle inside macrophage similar to the host. The in-vitro dynamic proliferation of the obligate intracellular protozoan parasites of L.tropica and L.major on peripheral mononuclear cells of five groups was investigated as follws: 1- control group(ten individuals) 2- Infected with L.tropica and sensitive to the treatmentve individuals) 3- Infected with L.major and sensitive to the treatment(five individuals) 4- Infected with L.tropica and resistant to the treatment (five individuals) 5-Infected with L.major and resistant to treatment(five individuals). Separated mononuclear cells of peripheral blood were infected with L.major and L.tropica and they were cultivated. far 14 days. In the days 1,3,5, 7,10, I4 after infection, the number of infected macrophages and the number of amastigots/macrophage have been counted. Using statistical trials t.test and paired t.test, no significant differences observed between two healthy groups. On the other hand, significant differences observed between resistant and control groups as well as sensitive and resistant groups(P<0. 05). Severe infection with L.major and L.tropica in both resistant groups has been shown in this study. The identification of the dynamic of proliferative cycle of L.tropica and L. major in human macrophages can help us. for successful treatment of cutaneous leismaniasis.